A5083736605- Neurogenesis and neuroplasticity mechanisms
- Epigenetics and DNA Methylation
- Congenital heart defects research
- Genetics and Neurodevelopmental Disorders
- Glioma Diagnosis and Treatment
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- Developmental Biology and Gene Regulation
- RNA Research and Splicing
- Spinal Dysraphism and Malformations
- Pancreatic function and diabetes
- Lymphatic System and Diseases
- Cancer, Hypoxia, and Metabolism
- Cerebrospinal fluid and hydrocephalus
- Neuroscience and Neuropharmacology Research
- Axon Guidance and Neuronal Signaling
- Urological Disorders and Treatments
- Reproductive Biology and Fertility
- Colorectal and Anal Carcinomas
- Zebrafish Biomedical Research Applications
- Immune Cell Function and Interaction
- Hedgehog Signaling Pathway Studies
- Kruppel-like factors research
- Sperm and Testicular Function
- Renal and related cancers
Cornell University
2021-2024
Weill Cornell Medicine
2022-2024
MIND Research Institute
2021-2024
New York Proton Center
2023
Randolph–Macon College
2023
Broad Center
2012-2022
University of California, Los Angeles
2012-2022
The University of Texas Southwestern Medical Center
2021
University of Sheffield
1997-2019
University of California, San Francisco
2005
During tissue development, multipotent progenitors differentiate into specific cell types in characteristic spatial and temporal patterns. We addressed the mechanism linking progenitor identity differentiation rate neural tube, where motor neuron (MN) more rapidly than other progenitors. Using single transcriptomics, we defined transcriptional changes associated with transition of MNs. Reconstruction gene expression dynamics from these data indicate a pivotal role for MN determinant Olig2...
The cardiac lymphatic vascular system and its potentially critical functions in heart patients have been largely underappreciated, part due to a lack of experimentally accessible systems. We here demonstrate that vessels develop young adult zebrafish, using coronary arteries guide their expansion down the ventricle. Mechanistically, we show cxcr4a mutants with defective artery development, fail expand onto In regenerating zebrafish hearts vasculature undergoes extensive lymphangiogenesis...
Telencephalic organoids generated from human pluripotent stem cells (hPSCs) are a promising system for studying the distinct features of developing brain and underlying causes many neurological disorders. While organoid technology is steadily advancing, challenges remain, including potential batch-to-batch cell-line-to-cell-line variability, structural inconsistency. Here, we demonstrate that major contributor to cortical quality way hPSCs maintained prior differentiation. Optimal results...
Cryopreservation at ultra-low temperatures is a valuable tool for preserving cells and tissues used in research. However, few protocols exist the preservation of brain organoid models. Current methods human cortical organoids (hCOs) rely on conventional slow cooling approaches with suspended medium containing cocktail cryoprotectants. In contrast, we have optimized vitrification technique previously to cryopreserve embryos oocytes application hCOs. We successfully cryopreserved hCOs that...
Object Tethering of the spinal cord is a pathological fixation in vertebral column that can result neurogenic bladder dysfunction and other neurological problems. It occurs patients with closed dysraphisms those whom postoperative scarring develops following spina bifida closure procedures. The authors this study sought to determine effects detethering on urodynamic profile children tethered cord. Methods retrospectively reviewed records who underwent surgical release at single institution...
The infundibulum links the nervous and endocrine systems, serving as a crucial integrating centre for body homeostasis. Here we describe that chick derives from two subsets of anterior ventral midline cells. One set remains at forms posterior-ventral infundibulum. A second migrates laterally, forming collar around midline. We show cells are composed Fgf3+ SOX3+ proliferating progenitors, induction which is SHH dependent, but maintenance requires FGF signalling. Collar proliferate late into...
The neurohypophysis is a crucial component of the hypothalamo-pituitary axis, serving as site release hypothalamic neurohormones into plexus hypophyseal capillaries. growth axons and capillaries to forming in embryogenesis therefore future adult homeostasis. Using ex vivo analyses chick mutant transgenic zebrafish, we show that Fgf10 Fgf3 secreted from exert direct guidance effects on neurosecretory axons. Simultaneously, they promote vascularisation, exerting early endothelial cells are...
Abstract Neural progenitor cells within the cerebral cortex undergo a characteristic switch between symmetric self-renewing cell divisions early in development and asymmetric neurogenic later. Yet, mechanisms controlling this transition remain unclear. Previous work has shown that but not late neural (NPCs) endogenously express autism-linked transcription factor Foxp1, both loss gain of Foxp1 function can alter NPC activity fate choices. Here, we show premature upregulates transcriptional...
ABSTRACT During tissue development, multipotent progenitors differentiate into specific cell types in characteristic spatial and temporal patterns. We address the mechanism linking progenitor identity differentiation rate neural tube, where motor neuron (MN) more rapidly than other progenitors. Using single transcriptomics, we define transcriptional changes associated with transition of MNs. Reconstruction gene expression dynamics from these data indicate a pivotal role for MN determinant...
SUMMARY The laminar architecture of the mammalian neocortex depends on orderly generation distinct neuronal subtypes by apical radial glia (aRG) during embryogenesis. We identify critical roles for Foxp1 in maintaining RG identity and gating temporal competency early neurogenesis. High levels are associated with aRG required to promote proliferation influence cell division symmetry, favoring expansion production born neurons. potent pro-progenitor functions revealed through its ability...
Abstract Glucocorticoids contribute to the daily migration patterns of T cells in well-nourished organisms and are elevated malnourished. We examined effect malnutrition on cell by comparing adoptively transferred malnourished control lymphoid organs recipients. found that generally entered tissues more efficiently than cells, regardless recipient. Strikingly, bone marrow recipients attracted naïve but not marrow. In contrast, spleens mice similar numbers cells. Further experiments revealed...
Background All Spina Bifida patients with Myelomeningocele require specialty care from Orthopaedics, Neurology, Urology, GI and Plastics. A high percentage of these surgery as a treatment modality. This outcome research study illustrated increased compilations post in VP shunts compared to the general population non-existing shunts. The special focus is on shunt placement for Hydrocephalus and/or Arnold Chiari Malformations. Although this specific patient may surgical intervention, it...