A5101422575- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Bioinformatics and Genomic Networks
- Pluripotent Stem Cells Research
- Gene Regulatory Network Analysis
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Gene expression and cancer classification
- Fungal and yeast genetics research
- Autism Spectrum Disorder Research
- 3D Printing in Biomedical Research
- MicroRNA in disease regulation
- Neuroscience and Neural Engineering
- Genomics and Chromatin Dynamics
- Geological Modeling and Analysis
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Circular RNAs in diseases
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Plant Reproductive Biology
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- Plant tissue culture and regeneration
- FOXO transcription factor regulation
Shanxi Agricultural University
2024
Novartis (United States)
2024
University of Michigan–Ann Arbor
2017-2024
Hunan Agricultural University
2024
University of Toledo
2023-2024
Michigan United
2024
Guangzhou University of Chinese Medicine
2022
VA Ann Arbor Healthcare System
2019
Sichuan University
2018
Jilin Agricultural Science and Technology University
2017
From Genome to Regulatory Networks For biologists, having a genome in hand is only the beginning—much more investigation still needed characterize how used help produce functional organism (see Perspective by Blaxter ). In this vein, Gerstein et al. (p. 1775 ) summarize for Caenorhabditis elegans genome, and The modENCODE Consortium 1787 Drosophila melanogaster full transcriptome analyses over developmental stages, genome-wide identification of transcription factor binding sites,...
Abstract Recent studies implicate chromatin modifiers in autism spectrum disorder (ASD) through the identification of recurrent de novo loss function mutations affected individuals. ASD risk genes are co-expressed human midfetal cortex, suggesting that converge specific regulatory networks during neurodevelopment. To elucidate such networks, we identify targeted by CHD8, a chromodomain helicase strongly associated with ASD, brain, neural stem cells (hNSCs) and embryonic mouse cortex. CHD8...
A map of genome-wide binding locations 165 human, 93 worm and 52 fly transcription-regulatory factors (almost 50% presented for the first time) from diverse cell types, developmental stages, or conditions reveals that gene-regulatory properties previously observed individual may be general principles metazoan regulation are well preserved. Fly have long been studied as key models human biology. Conservation gene has until now mainly by focusing on elements factors. It goal modENCODE...
Sickle cell disease (SCD) is a prevalent, life-threatening condition attributable to heritable mutation in β-hemoglobin. Therapeutic induction of fetal hemoglobin (HbF) can ameliorate complications and has been intently pursued. However, safe effective small-molecule inducers HbF remain elusive. We report the discovery dWIZ-1 dWIZ-2, molecular glue degraders WIZ transcription factor that robustly induce erythroblasts. Phenotypic screening cereblon (CRBN)-biased chemical library revealed as...
Regulation of gene expression by sequence-specific transcription factors is central to developmental programs and depends on the binding with target sites in genome. To date, most such analyses Caenorhabditis elegans have focused interactions between a single factor one or few select genes. As part modENCODE Consortium, we used chromatin immunoprecipitation coupled high-throughput DNA sequencing (ChIP-seq) determine genome-wide 22 (ALR-1, BLMP-1, CEH-14, CEH-30, EGL-27, EGL-5, ELT-3, EOR-1,...
Shawn T. Estrem, Wilma Ross, Tamas Gaal, Z. W. Susan Chen, Wei Niu, Richard H. Ebright, and L. Gourse Department of Bacteriology, University Wisconsin, Madison, Wisconsin 53706 USA; Howard Hughes Medical Institute, Waksman Institute Chemistry, Rutgers University, Piscataway, New Jersey 08854 USA
Transcription factors are key components of regulatory networks that control development, as well the response to environmental stimuli. We have established an experimental pipeline in Caenorhabditis elegans permits global identification binding sites for transcription using chromatin immunoprecipitation and deep sequencing. describe validate this strategy, apply it factor PHA-4, which plays critical roles organ development other cellular processes. identified thousands PHA-4 during...
Discovering the structure and dynamics of transcriptional regulatory events in genome with cellular temporal resolution is crucial to understanding underpinnings development disease. We determined genomic distribution binding sites for 92 transcription factors proteins across multiple stages Caenorhabditis elegans by performing 241 ChIP-seq (chromatin immunoprecipitation followed sequencing) experiments. Integration cellular-resolution expression data produced a spatiotemporally resolved...
Brain organoid methods are complicated by multiple rosette structures and morphological variability. We have developed a human brain technique that generates self-organizing, single-rosette cortical organoids (SOSR-COs) with reproducible size structure at early timepoints. Rather than patterning 3-dimensional embryoid body, we initiate formation from 2-dimensional monolayer of pluripotent stem cells patterned small molecules into neuroepithelium differentiated to the developing dorsal...
We present a network framework for analyzing multi-level regulation in higher eukaryotes based on systematic integration of various high-throughput datasets. The network, namely the integrated regulatory consists three major types regulation: TF→gene, TF→miRNA and miRNA→gene. identified target genes miRNAs set TFs ChIP-Seq binding profiles, predicted targets using annotated 3′UTR sequences conservation information. Making use system-wide RNA-Seq we classified transcription factors into...
Regulation of cell cycle progression is fundamental to health and reproduction, failures in this process are associated with many human diseases. Much our knowledge regulators derives from loss-of-function studies. To reveal new regulatory genes that difficult identify studies, we performed a near-genome-wide flow cytometry assay yeast gene overexpression-induced delay phenotypes. We identified 108 whose overexpression significantly delayed the at specific stage. Many newly implicated...
Abstract Background The tumor suppressor Rb/E2F regulates gene expression to control differentiation in multiple tissues during development, although how it directs tissue-specific regulation vivo is poorly understood. Results We determined the genome-wide binding profiles for Caenorhabditis elegans Rb/E2F-like components germline, intestine and broadly throughout soma, uncovered highly patterns target genes. Chromatin association by LIN-35, C. ortholog of Rb, impaired germline but robust a...
We have developed spotted cell microarrays for measuring cellular phenotypes on a large scale. Collections of cells are printed, stained subcellular features, then imaged via automated, high-throughput microscopy, allowing systematic phenotypic characterization. used this technology to identify genes involved in the response yeast mating pheromone. Besides morphology assays, should be valuable situ hybridization and immunoassays, enabling new classes genetic assays based imaging.
ABSTRACT The field of brain organoid research is complicated by morphological variability with multiple neural rosette structures per organoid. We have developed a new human technique that generates self-organizing, single-rosette spheroids (SOSRS) reproducible size, cortical-like lamination, and cell diversity. Rather than patterning 3-dimensional embryoid body, we initiate formation from 2-dimensional monolayer pluripotent stem cells (hPSCs) patterned small molecules into neuroepithelium...
To investigate the effects of visual feedback training on knee function and balance ability in postoperative patients with fracture.A clinical randomized controlled trial 104 participants, randomly assigned (1:1) to a group or control group. Eligible participants were evaluated using Lysholm scores, Tinetti scale, evaluation module TecnoBody® demonstrate dynamic standing position before treatment after 4 8 weeks treatment.Both groups improved significantly scores weeks. Compared group, tests...
Polarizing cells extensively restructure cellular components in a spatially and temporally coupled manner along the major axis of extension. Budding yeast are useful model polarized growth, helping to define many molecular this conserved process. Besides budding, also differentiate upon treatment with pheromone from opposite mating type, forming projection (the 'shmoo') by directional restructuring cytoskeleton, localized vesicular transport overall reorganization cytosol. To characterize...