Mihovil Pletikos
A5044641544- Neonatal and fetal brain pathology
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Advanced Neuroimaging Techniques and Applications
- Fetal and Pediatric Neurological Disorders
- Single-cell and spatial transcriptomics
- Genetics and Neurodevelopmental Disorders
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Advanced MRI Techniques and Applications
- Neural dynamics and brain function
- MicroRNA in disease regulation
- Mosquito-borne diseases and control
- Genomics and Chromatin Dynamics
- Gene expression and cancer classification
- Functional Brain Connectivity Studies
- Memory and Neural Mechanisms
- Viral Infections and Vectors
- Connective tissue disorders research
- Genomic variations and chromosomal abnormalities
- Advanced Electron Microscopy Techniques and Applications
- Hemispheric Asymmetry in Neuroscience
- Pluripotent Stem Cells Research
- Cerebrospinal fluid and hydrocephalus
Yale University
2011-2025
Boston University
2018
University of Zagreb
2010-2016
INTRODUCTION The brain is responsible for cognition, behavior, and much of what makes us uniquely human. development the a highly complex process, this process reliant on precise regulation molecular cellular events grounded in spatiotemporal transcriptome. Disruption can lead to neuropsychiatric disorders. RATIONALE regulatory, epigenomic, transcriptomic features human have not been comprehensively compiled across time, regions, or cell types. Understanding etiology disorders requires...
Abstract Recent studies implicate chromatin modifiers in autism spectrum disorder (ASD) through the identification of recurrent de novo loss function mutations affected individuals. ASD risk genes are co-expressed human midfetal cortex, suggesting that converge specific regulatory networks during neurodevelopment. To elucidate such networks, we identify targeted by CHD8, a chromodomain helicase strongly associated with ASD, brain, neural stem cells (hNSCs) and embryonic mouse cortex. CHD8...
The mechanisms underlying Zika virus (ZIKV)-related microcephaly and other neurodevelopment defects remain poorly understood. Here, we describe the derivation characterization, including single-cell RNA-seq, of neocortical spinal cord neuroepithelial stem (NES) cells to model early human ZIKV-related neuropathogenesis. By analyzing NES cells, organotypic fetal brain slices, a ZIKV-infected micrencephalic brain, show that ZIKV infects both as well their homolog, radial glial (RGCs), causing...
Somatic mosaicism in the human brain may alter function of individual neurons. We analyzed genomes single cells from forebrains three fetuses (15 to 21 weeks postconception) using clonal cell populations. detected 200 400 single-nucleotide variations (SNVs) per cell. SNV patterns resembled those found cancer genomes, indicating a role background mutagenesis cancer. SNVs with frequency >2% were also present spleen, revealing pregastrulation origin. reconstructed lineages for first five...
The makings of the primate brain Although nonhuman brains are similar to our own, disparity between their and cognitive abilities tells us that surface similarity is not whole story. Sousa et al. overlaid transcriptome histological analyses see what makes human different from those primates. Various differentially expressed genes, such as encoding transcription factors, could alter transcriptional programs. Others were associated with neuromodulatory systems. Furthermore, dopaminergic...
A sheet of choroid plexus epithelial cells extends into each cerebral ventricle and secretes signaling factors the CSF. To evaluate whether differences in CSF proteome across ventricles arise, part, from regional gene expression, we defined transcriptome lateral (telencephalic) versus fourth (hindbrain) plexus. We find that positional identities mouse, macaque, human plexi derive expression domains parallel their axial tissues origin. then show molecular heterogeneity between telencephalic...
Significance This study of the monkey cerebral cortex revealed age-related changes that help to answer key questions about Alzheimer’s disease (AD): ( i ) why advancing age is highest risk factor for AD and ii neurofibrillary tangles (NFTs) in selectively target extensively interconnected pyramidal neurons highly evolved association cortex, but do not afflict primary sensory cortex. NFTs arise from phosphorylation tau protein. In aged prefrontal we found increased cAMP-dependent protein...
The cerebral wall of the human fetal brain is composed transient cellular compartments, which show characteristic spatiotemporal relationships with intensity major neurogenic events (cell proliferation, migration, axonal growth, dendritic differentiation, synaptogenesis, cell death, and myelination). aim present study was to obtain new quantitative data describing volume, surface area, thickness compartments in cerebrum. Forty-four postmortem brains aged 13-40 postconceptional weeks (PCW)...
Abstract The ventrolateral pallial (VLp) excitatory neurons in the claustro-amygdalar complex and piriform cortex (PIR; which forms part of palaeocortex) form reciprocal connections with prefrontal (PFC), integrating cognitive sensory information that results adaptive behaviours 1–5 . Early-life disruptions these circuits are linked to neuropsychiatric disorders 4–8 , highlighting importance understanding their development. Here we reveal transcription factors SOX4, SOX11 TFAP2D have a...
As a prominent component of the human fetal brain, structure cerebral wall is characterized by its laminar organization which includes radial glial scaffold during development. Diffusion tensor imaging (DTI) useful to quantitatively delineate microstructure developing brain and clearly identify transient layers in wall. In our study, spatio-temporal microstructural changes were with high-resolution DTI data postmortem brains from 13 21 gestational weeks. Eleven regions interest for each...