A5073046516- Glycosylation and Glycoproteins Research
- Mitochondrial Function and Pathology
- Carbohydrate Chemistry and Synthesis
- Neuroscience and Neuropharmacology Research
- Ubiquitin and proteasome pathways
- Nuclear Receptors and Signaling
- Machine Learning in Bioinformatics
- Mechanisms of cancer metastasis
- Galectins and Cancer Biology
- RNA modifications and cancer
- Memory and Neural Mechanisms
- Proteoglycans and glycosaminoglycans research
- Functional Brain Connectivity Studies
- ATP Synthase and ATPases Research
- Stress Responses and Cortisol
- Neuroinflammation and Neurodegeneration Mechanisms
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- Microbial Metabolites in Food Biotechnology
- Optical Imaging and Spectroscopy Techniques
- Signaling Pathways in Disease
- Genomic variations and chromosomal abnormalities
- Advanced Neuroimaging Techniques and Applications
- Tryptophan and brain disorders
Yale University
2023-2025
Genos (Croatia)
2016-2024
Science Research Laboratory
2020-2024
University of New Haven
2023
University of Zagreb
2020
The University of Adelaide
2008-2017
Abstract The ventrolateral pallial (VLp) excitatory neurons in the claustro-amygdalar complex and piriform cortex (PIR; which forms part of palaeocortex) form reciprocal connections with prefrontal (PFC), integrating cognitive sensory information that results adaptive behaviours 1–5 . Early-life disruptions these circuits are linked to neuropsychiatric disorders 4–8 , highlighting importance understanding their development. Here we reveal transcription factors SOX4, SOX11 TFAP2D have a...
Stroke is the second leading cause of death and most frequent adult disability. Neuronal Per-Arnt-Sim domain protein 4 (Npas4) an activity-dependent transcription factor whose expression induced in various brain insults, including cerebral ischaemia. Although previous studies have demonstrated that Npas4 plays a critical role protecting neurons against neurodegenerative neuroprotective effect response to ischaemic injury remains unknown. In this study, we used loss-of-function approach...
Comparative "omics" studies have revealed unique aspects of human neurobiology, yet an evolutionary perspective the brain N-glycome is lacking. We performed multiregional characterization rat, macaque, chimpanzee, and N-glycomes using chromatography mass spectrometry then integrated these data with complementary glycotranscriptomic data. found that, in primates, has diverged more rapidly than underlying transcriptomic framework, providing a means for generating additional interspecies...
Abstract Human protein glycosylation is a complex process, and its in vivo regulation poorly understood. Changes patterns are associated with many human diseases conditions. Understanding the biological determinants of glycome provides basis for future diagnostic therapeutic applications. Genome-wide association studies (GWAS) allow to study biology via hypothesis-free search loci genetic variants trait interest. Sixteen were identified by three previous GWAS plasma proteome N-glycosylation....
Abstract The neuronal P er‐ A rnt‐ S im domain protein 4 ( N pas4) is an important transcriptional regulator of synaptic plasticity and cognition. present study characterises the in vivo neuroanatomical expression pattern pas4 a rat model focal cerebral ischemia. Animals were subjected to unilateral middle artery occlusion for 2 h, after which spatiotemporal profiles analysed by immunohistochemistry at different time points post‐reperfusion. Focal ischemia induced early, transient robust...
We report here on the development of a method for inducing stroke in specific location within mouse brain through use an optical fiber.By capturing emitted fluorescence signal generated using same fiber it is possible to monitor photochemical changes real-time, and directly measure concentration strokeinducing dye, Rose Bengal, at infarct site.This technique reduces requirement post-operative histology determine if has successfully been induced animal, therefore opens up opportunity explore...
Asparagine-linked glycosylation (N-glycosylation) plays a key role in many neurodevelopmental processes, including neural cell adhesion, neurite outgrowth and axon targeting. However, little is known about the dynamics of N-glycosylation during brain development and, particular, how N-glycome developing neocortex differs from that adult. The aim this study, therefore, was to perform thorough characterization both adult neonatal rat order gain insights into types changes occurring...
Abstract The ventropallial excitatory neurons (ExNs) in the claustro-amygdalar complex and piriform cortex (PIR; part of paleocortex) form crucial reciprocal connections with prefrontal (PFC), integrating cognitive sensory information that result adaptive behaviors. Early-life disruptions these circuits are linked to neuropsychiatric disorders, highlighting importance understanding their development. Our study uncovers transcription factors SOX4, SOX11, TFAP2D play a pivotal role...
Npas4 is a calcium-dependent transcription factor expressed within neurons of the brain where it regulates expression several genes that are important for neuronal survival and synaptic plasticity. It known in adult plays an role key aspects neurobiology including inhibitory synapse formation, neuroprotection memory, yet very little about during neurodevelopment. The aim this study was to examine function nervous system development by using combination vivo experiments developing mouse...
Posttraumatic stress disorder (PTSD) is triggered by traumatic events in 10–20% of exposed subjects. N-linked glycosylation, modifying protein functions, may provide an important environmental link predicting vulnerability. Our goals were (1) to find alterations plasma N-glycome stress-vulnerability; (2) investigate how trauma affects the (PGP) and three PTSD-related brain regions (prefrontal cortex, hippocampus amygdala; BGP), hence, uncover specific targets for PTSD treatment. We examined...
Lysate in-Solution Deglycosylation (LSD) is a novel method for the preparation of <italic>N</italic>-glycans from tissue. The substrate LSD commonly used lysate that starting point many other applications, including classical proteomics methods.
Invertases are glycosidases applied for synthesis of alkyl glycosides that important and effective surfactants. Stability invertases in the environment with increased content organic solvent is crucial increase productivity glycosidases. Their stability significantly influenced by N-glycosylation. However, yeast N-glycosylation pathways may synthesize plethora N-glycan structures. A total natural crude mixture invertase glycoforms (EINV) extracted from Saccharomyces cerevisiae was...
Down syndrome (DS), a genetic condition caused by trisomy 21 (T21), manifests various neurological symptoms, including intellectual disability, early neurodegeneration, and early-onset dementia. N-glycosylation is protein modification that plays critical role in numerous neurobiological processes whose dysregulation associated with range of disorders. However, whether neural glycoproteins affected DS has not been studied. To better understand how T21 affects during differentiation, we...
ABSTRACT Down syndrome (DS) is a genetic condition caused by trisomy 21 (T21) and characterized range of neurological symptoms including intellectual disability, early onset neurodegeneration dementia, some which can be attributed to accelerated aging. N-glycosylation protein modification that plays critical role in numerous biological processes its dysregulation associated with wide diseases, even before the symptoms. total plasma proteins, as well specific such immunoglobulin G, has been...
Comparative "omics" studies have revealed unique aspects of human neurobiology, yet an evolutionary perspective the brain N-glycome is lacking. Here, we performed multi-regional characterization rat, macaque, chimpanzee, and N-glycomes using chromatography mass spectrometry, then integrated these data with complementary glycotranscriptomic data. We found that in primates has evolved more rapidly than underlying transcriptomic framework, providing a mechanism for generating additional...
ABSTRACT Autism Spectrum Disorders (ASDs) are a heterogeneous collection of neurodevelopmental disorders with strong genetic basis. Recent studies identified that single hit either de novo or transmitted gene-disrupting, likely mutation in subset 65 strongly associated genes can be sufficient to generate an ASD phenotype. We took advantage consanguineous families proband evaluate this model. By genome-wide homozygosity mapping ten eleven children displaying ASD, we linkage region 133 kb five...
We report here on the development of a method to induce stroke in specific location within mouse brain through use an optical fibre. By capturing emitted fluorescence signal generated using same fibre it is then possible monitor photochemical changes real-time, potentially reducing requirement for post-operative histology determine if has successfully been induced animal.