A5100670137- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Genetics and Neurodevelopmental Disorders
- Cancer-related gene regulation
- Prenatal Screening and Diagnostics
- Single-cell and spatial transcriptomics
- Immune cells in cancer
- MicroRNA in disease regulation
- Chromosomal and Genetic Variations
- Genomics and Chromatin Dynamics
- Circular RNAs in diseases
- Histone Deacetylase Inhibitors Research
- Cancer, Lipids, and Metabolism
- RNA Research and Splicing
- Renal and related cancers
- Genetics, Aging, and Longevity in Model Organisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Geological Modeling and Analysis
- Fatty Acid Research and Health
- Heavy metals in environment
- Pharmacological Effects of Natural Compounds
- Pregnancy and preeclampsia studies
Yunnan University
2023-2024
Southern Medical University
2014-2024
Yale Cancer Center
2015-2024
Yunnan Institute of Environmental Sciences
2024
Yale University
2010-2024
Nanfang Hospital
2014-2024
Shenzhen Luohu People's Hospital
2023
Nanjing University of Chinese Medicine
2022-2023
Chinese University of Hong Kong
2022-2023
Southwest University
2019-2021
Induced pluripotent stem cell (iPS) technology appears to be a general strategy generate cells from any given mammalian species. So far, iPS have been reported for mouse, human, rat, and monkey. These four species also established embryonic (ESC) lines that serve as the gold standard pluripotency comparisons. Attempts made porcine ESC by various means without success. Here we report successful generation of fibroblasts isolated Tibetan miniature pig using modified protocol. The resulting...
The eukaryotic genome has vast intergenic regions containing transposons, pseudogenes, and other repetitive sequences. They produce numerous long noncoding RNAs (lncRNAs) Piwi-interacting (piRNAs), yet the functions of remain largely unknown. Mammalian piRNAs are abundantly expressed from spermatocyte to round spermatid stage, coinciding with widespread expression lncRNAs in these cells. Here, we show that derived transposons pseudogenes mediate degradation a large number mRNAs mouse late...
Microglia play a role in the emergence and preservation of healthy brain microenvironment. Dysfunction microglia has been associated with neurodevelopmental neurodegenerative disorders. Investigating function human health disease challenging due to limited models available. Here, we develop method generate functional cortical organoids (hCOs) from embryonic stem cells (hESCs). We apply this system study during inflammation induced by amyloid-β (Aβ). The overexpression myeloid-specific...
Regulation of gene expression by sequence-specific transcription factors is central to developmental programs and depends on the binding with target sites in genome. To date, most such analyses Caenorhabditis elegans have focused interactions between a single factor one or few select genes. As part modENCODE Consortium, we used chromatin immunoprecipitation coupled high-throughput DNA sequencing (ChIP-seq) determine genome-wide 22 (ALR-1, BLMP-1, CEH-14, CEH-30, EGL-27, EGL-5, ELT-3, EOR-1,...
The umbilical cord and placenta are extra-embryonic tissues of particular interest for regenerative medicine. They share an early developmental origin a source vast amounts cells with multilineage differentiation potential that poorly immunogenic without controversy. Moreover, these likely exempt from incorporated mutations when compared juvenile or adult donor such as skin fibroblasts keratinocytes. Here we report the efficient generation induced pluripotent stem (iPSCs) mesenchymal matrix...
Transcription factors are key components of regulatory networks that control development, as well the response to environmental stimuli. We have established an experimental pipeline in Caenorhabditis elegans permits global identification binding sites for transcription using chromatin immunoprecipitation and deep sequencing. describe validate this strategy, apply it factor PHA-4, which plays critical roles organ development other cellular processes. identified thousands PHA-4 during...
PIWI proteins and their bound PIWI-interacting RNAs (piRNAs) are found in animal germlines essential for fertility, but functions outside of the gonad not well understood. The cnidarian Hydra is a simple metazoan with well-characterized stem/progenitor cells that provides unique model analysis function. Here we report has two proteins, (Hywi) PIWI-like (Hyli), both which expressed all cells, terminally differentiated cells. We identified ∼15 million piRNAs associated Hywi and/or Hyli they...
Despite exciting progress in understanding the Piwi-interacting RNA (piRNA) pathway germ line, less is known about this somatic cells. We showed previously that Piwi, a key component of piRNA <i>Drosophila</i>, regulated cells by Yb, novel protein containing an helicase-like motif and Tudor-like domain. <i>Yb</i> specifically expressed gonadal regulates <i>piwi</i> niche to control line stem cell self-renewal. However, molecular basis regulation remains elusive. Here, we report Yb recruits...
Steady state cellular microRNA (miRNA) levels represent the balance between miRNA biogenesis and turnover. The kinetics sequence determinants of mammalian turnover during after maturation are not fully understood. Through a large-scale study on turnover, we report co-existence multiple pools with distinct properties reveal previously unrecognized features for fast miRNAs. We measured rates in eight cell types combination expression profiling deep sequencing. While most miRNAs stable, subset...
Abstract MicroRNAs have implicated in the relapse and metastasis of cervical cancer, which is leading cause cancer-related mortality. However, underlying molecular mechanisms need further elucidation. Our present study revealed that miR-221-3p transcriptionally promoted metastatic cancer tissues compared with non-metastatic tissues. Forced overexpression facilitated EMT cell migration invasion vitro lymphatic vivo . Twist homolog 2 (TWIST2) was found to be a key transcription factor binding...
To explore the mechanisms through which hypoxic tumor microenvironment (TME) modulates transition of tumor‐associated macrophages (TAMs). The migration ability RAW264.7 was determined by transwell assay. Flow cytometric, western blot and immunofluorescence analyses CD206 further validated M2 polarization macrophages. Immunofluorescence, qRT‐PCR were performed to detect expression neuropilin‐1 (Nrp‐1) carbonic anhydrase IX (CAIX). An intermittent hypobaric hypoxia (IH) animal model...
Abstract The ability to spatially map multiple layers of the omics information over different time points allows for exploring mechanisms driving brain development, differentiation, arealization, and alterations in disease. Herein we developed applied spatial tri-omic sequencing technologies, DBiT ARP-seq (spatial ATAC–RNA–Protein-seq) CTRP-seq CUT&Tag– RNA–Protein-seq) together with multiplexed immunofluorescence imaging (CODEX) dynamic remodeling development neuroinflammation. A...
The recent discovery of induced pluripotent stem cell (iPSC) technology provides an invaluable tool for creating in vitro representations human genetic conditions. This is particularly relevant those diseases that lack adequate animal models or where the species comparison difficult, e.g. imprinting such as neurogenetic disorder Prader-Willi syndrome (PWS). However, reports have unveiled transcriptional and functional differences between iPSCs embryonic cells cases are attributable to...
Objective.To explore the influence of M2-polarized tumor-associated macrophages (TAMs) on high-risk human papillomavirus (hr-HPV)-related cervical carcinogenesis and metastasis.Methods.CD68+ CD163+ were examined immunohistochemically in a series 130 samples, including 26 cases normal tissues, 59 intraepithelial neoplasia (CIN), 45 squamous cell carcinoma (SCC), results statistically analyzed.The macrophage count was corrected for epithelial stromal compartments respectively.Clinical data...