A5075850032- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Endometriosis Research and Treatment
- CRISPR and Genetic Engineering
- Graphene and Nanomaterials Applications
- Microtubule and mitosis dynamics
- Neuroscience and Neuropharmacology Research
- Uterine Myomas and Treatments
- Bone Tissue Engineering Materials
- Neurogenesis and neuroplasticity mechanisms
- Reproductive Biology and Fertility
- Additive Manufacturing and 3D Printing Technologies
- Renal and related cancers
- Cell Image Analysis Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Graphene research and applications
- Tissue Engineering and Regenerative Medicine
- Assisted Reproductive Technology and Twin Pregnancy
- Immune cells in cancer
- Endometrial and Cervical Cancer Treatments
- Axon Guidance and Neuronal Signaling
- Single-cell and spatial transcriptomics
- Wnt/β-catenin signaling in development and cancer
- Neonatal Respiratory Health Research
- Ovarian function and disorders
Yale Cancer Center
2022-2024
Seoul National University
2014-2020
Microglia play a role in the emergence and preservation of healthy brain microenvironment. Dysfunction microglia has been associated with neurodevelopmental neurodegenerative disorders. Investigating function human health disease challenging due to limited models available. Here, we develop method generate functional cortical organoids (hCOs) from embryonic stem cells (hESCs). We apply this system study during inflammation induced by amyloid-β (Aβ). The overexpression myeloid-specific...
Regular monitoring on experimental animal management found the fluctuation of ART outcome, which showed a necessity to explore whether superovulation treatment is responsible for such unexpected outcome.This study was subsequently conducted examine can preserve ultrastructural integrity and developmental competence oocytes following oocyte activation embryo culture.A randomized using mouse model designed in vitro development (experiment 1), morphology 2) functional 3) retrieved after...
Adenomyosis is defined as the presence of ectopic nests endometrial glands and stroma within myometrium. a common cause dysmenorrhea, menorrhagia, chronic pelvic pain but often underdiagnosed. Despite its prevalence severity symptoms, pathogenesis etiology are poorly understood. Our previous study showed that aberrant activation β-catenin results in adenomyosis through epithelial-mesenchymal transition. Using transcriptomic ChIP-seq analysis, we identified TGF-β signaling uteri mutant mice...
The telencephalon is the largest region of brain and processes critical activity. Despite much progress, our understanding telencephalon’s function, development, pathophysiological remains largely incomplete. Recently, 3-dimensional models, known as organoids, have attracted considerable attention in modern neurobiological research. Brain organoids been proven to be valuable for studying neurodevelopmental principles pathophysiology brain, well developing potential therapeutics. can change...
This study examined whether neonatal chicken bone marrow cells (cBMCs) could support the osteogenesis of human stromal in a three-dimensional (3D) extracellular bioprinting niche. The majority (>95%) 4-day-old cBMCs subcultured 5 times were positive for osteochondrogenesis-related genes (Col I, Col II, X, aggrecan, Sox9, osterix, Bmp2, osteocalcin, Runx2, and osteopontin) their related proteins (Sox9, collagen type II). LC–MS/MS analysis demonstrated that cBMC-conditioned medium (c-medium)...
The aim of this study was to assess the biological reactions triggered by stem cell transplantation related phenotypic alteration, host-to-cell response, chromosomal stability, transcriptional and cell-like re-expansion. B6CBAF1 mouse embryonic cells (ESCs) were injected subcutaneously into homologous or heterologous (B6D2F1) recipients, injections performed with without co-injection B6D2F1 fetal fibroblasts. All resulted in teratoma formation, whereas a sharp decrease formation detected...
Abstract This study investigated whether hydroxyapatite (HAp)-mineralized graphene (GR) film could support osteogenic differentiation of human adipose-derived, stromal cell (hASCs) in vitro. GR was produced by a chemical vapor deposition (CVD) method and the physical characteristics film, which functionalized with HAp mineralization following ultraviolet-ozone (GR_UVO) treatment, were subsequently validated. Results scanning electron microscopy, x-ray photoelectron spectroscopy Raman showed...
Objective Remarkable difference in cellular activity was found between early and late subpassaged embryonic stem cell (ESCs) lines, which can be created by subtle changes manipulation protocol. This study subsequently examined whether post-thaw subculture of ESC lines could further affect the ESCs. Methods Fresh (as a control treatment) or cryopreserved F1 hybrid (B6CBAF1) (C57BL/6xCBA) 4 (P4) 19 passage (P19) were subcultured once, twice six times under same condition. The survival ESCs...
We evaluated whether the genetic background of embryonic stem cells (ESCs) affects properties suitable for three-dimensional (3D) synthetic scaffolds cell self-renewal. Inbred R1 and hybrid B6D2F1 mouse ESC lines were cultured 7 days in hydrogel with different derived from conjugating 7.5, 10, 12.5, or 15% (wt/vol) vinylsulfone-functionalized three-, four-, eight-arm polyethylene glycol (PEG) dicysteine-containing crosslinkers an intervening matrix metalloproteinase-specific cleavage sites....
Abstract Based on the concept that microenvironment and physical stimuli regulate cell behaviors like proliferation, migration, differentiation, this study was conducted to investigate whether nanoscale spacing by stacked graphene film affect osteogenic differentiation of human adipose-derived mesenchymal stromal cells (hADSCs). The films were synthesized a chemical vapor deposition method, followed etching rinsing process fabricate single or 3-, 5-, 7-multilayers. height width wrinkles...