A5100389807- CRISPR and Genetic Engineering
- Viral gastroenteritis research and epidemiology
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Electrospun Nanofibers in Biomedical Applications
- Telomeres, Telomerase, and Senescence
- Tissue Engineering and Regenerative Medicine
- Animal Virus Infections Studies
- Renal and related cancers
- Wound Healing and Treatments
- Chromosomal and Genetic Variations
- RNA Interference and Gene Delivery
- Psoriasis: Treatment and Pathogenesis
- Respiratory viral infections research
- RNA Research and Splicing
- RNA modifications and cancer
- Atrial Fibrillation Management and Outcomes
- Reproductive Biology and Fertility
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Cardiovascular Disease and Adiposity
Chinese PLA General Hospital
2013-2025
Central South University
2017-2025
Foshan University
2025
China Animal Health and Epidemiology Center
2023-2025
Xiangya Hospital Central South University
2022-2025
National Institute for Viral Disease Control and Prevention
2009-2024
Chinese Center For Disease Control and Prevention
2007-2024
Nanjing University
2023-2024
Nankai University
2015-2024
Qingdao University
2021-2024
Mesenchymal stem cell (MSC)-derived exosomes have been recognized as new candidates for cell-free treatment of various diseases. However, maintaining the retention and stability over time in vivo after transplantation is a major challenge clinical application MSC-derived exosomes. Here, we investigated if human placenta-derived incorporated with chitosan hydrogel could boost further enhance their therapeutic effects. Our results demonstrated that notably increased proteins microRNAs...
Hemoglobinopathies are among the most common autosomal-recessive disorders worldwide. A comprehensive next-generation sequencing (NGS) test would greatly facilitate screening and diagnosis of these disorders. An NGS panel targeting coding regions hemoglobin genes four modifier was designed. We validated assay by using 2522 subjects affected with hemoglobinopathies applied it to carrier testing in a cohort 10,111 couples who were also screened through traditional methods. In clinical...
Abstract The severity of osteoarthritis (OA) and cartilage degeneration is highly associated with synovial inflammation. Although recent investigations have revealed a dysregulated crosstalk between fibroblast‐like synoviocytes (FLSs) macrophages in the pathogenesis synovitis, limited knowledge available regarding involvement exosomes. Here, increased exosome secretion observed FLSs from OA patients. Notably, internalization inflammatory FLS‐derived exosomes (inf‐exo) can enhance M1...
BackgroundOral antiviral drugs with improved potency and safety are needed to address current challenges in clinical practice for treatment of COVID-19, including the risks rebound, drug-drug interactions, emerging resistance.MethodsOlgotrelvir (STI-1558) is designed as a next-generation targeting SARS-CoV-2 main protease (Mpro), an essential enzyme replication, human cathepsin L (CTSL), key entry into host cells.FindingsOlgotrelvir highly bioavailable oral prodrug that converted plasma its...
Periodontal ligament stem cells (PDLSCs), a new population of mesenchymal (MSCs), have been isolated from the periodontal (PDL). The capacity multipotency and self-renewal makes them an excellent cell source for bone regeneration repair. However, their bone-regeneration ability could be awakened in inflammatory microenvironments, which may result changes differentiation potential. Recently, genetic evidences has shown that Wnt pathway plays important role homeostasis. In this study we...
Abstract Chronic inflammatory diseases, such as rheumatoid arthritis and periodontitis, are the most common causes of bone tissue destruction. Recently, human periodontal ligament tissue-derived mesenchymal stem cells (PDLSCs), a population multipotent cells, have been used to reconstruct tissues destroyed by chronic inflammation. However, impact local microenvironment on tissue-specific mechanisms controlling effects environment remain poorly understood. In this study, we found that...
BACKGROUNDOvarian aging is associated with declining numbers and quality of oocytes follicles. Oxidative stress by reactive oxygen species (ROS) contributes to somatic in general, also has been implicated reproductive aging. Telomere shortening involved aging, telomeres are particularly susceptible ROS-induced damage. Previously, we have shown that antioxidant N-acetyl-l-cysteine (NAC) effectively rescues embryos from telomere apoptosis vitro. Using mice as models, tested the hypothesis...
PIWI proteins and their bound PIWI-interacting RNAs (piRNAs) are found in animal germlines essential for fertility, but functions outside of the gonad not well understood. The cnidarian Hydra is a simple metazoan with well-characterized stem/progenitor cells that provides unique model analysis function. Here we report has two proteins, (Hywi) PIWI-like (Hyli), both which expressed all cells, terminally differentiated cells. We identified ∼15 million piRNAs associated Hywi and/or Hyli they...
Angiotensin (Ang II) plays an important role in atherosclerosis through proinflammatory effect. Toll-like receptor 4 (TLR4) may mediate inflammatory response. It is unknown whether TLR4 mediates the effect of Ang II. Thus, we observed and signaling pathway II-induced inflammation rat vascular smooth muscle cells (VSMCs). II LPS stimulated TNF-α secretion inhibited 6-keto-PGF1α production, upregulated MMP-9 downregulated PPARγ PPARαin VSMCs. also distinctly expression cells. Pretreatment with...
Despite exciting progress in understanding the Piwi-interacting RNA (piRNA) pathway germ line, less is known about this somatic cells. We showed previously that Piwi, a key component of piRNA <i>Drosophila</i>, regulated cells by Yb, novel protein containing an helicase-like motif and Tudor-like domain. <i>Yb</i> specifically expressed gonadal regulates <i>piwi</i> niche to control line stem cell self-renewal. However, molecular basis regulation remains elusive. Here, we report Yb recruits...
Src-homology 2 domain-containing phosphatase (Shp2) has been reported to play an important role in the maintenance and self-renewal of embryonic adult stem cells, but its cancer cells (CSCs) remains obscure. Herein, we observed high expression Shp2 both chemoresistant hepatocellular carcinomas (HCCs) recurrent HCCs from patients. A remarkable increase was detected sorted epithelial cell adhesion molecule-positive or cluster differentiation 133-positive liver CSCs CSC-enriched hepatoma...
Background: Mesenchymal stem cell (MSC)-based therapies hold great promise for the treatment of inflammatory bowel disease (IBD). In order to optimize and maximize therapeutic benefits MSCs, we investigated whether cotransplantation a chitosan (CS)-based injectable hydrogel with immobilized IGF-1 C domain peptide (CS-IGF-1C) human placenta-derived MSCs (hP-MSCs) could ameliorate colitis in mice. Methods: IGF-1C was generated by immobilizing CS hydrogel. Colitis induced 2,4,6-trinitrobenzene...
Low cell retention and engraftment after transplantation limit the successful application of stem therapy for AKI. Engineered microenvironments consisting a hydrogel matrix growth factors have been increasingly in controlling fate by mimicking native niche components. Here, we synthesized bioactive immobilizing C domain peptide IGF-1 (IGF-1C) on chitosan, hypothesized that this could provide favorable adipose-derived mesenchymal cells (ADSCs) thereby enhance survival an AKI model. In vitro...
Lilium spp. is a bulb flower with worldwide distribution and unique underground organs. The lack of an efficient genetic transformation system for has been international obstacle. Because existing model plants bulbs, bulb-related gene function verification studies cannot be carried out in plants. Here, two stable systems based on somatic embryogenesis adventitious bud regeneration were established species. Transgenic T-DNA insertion lines confirmed by β-glucuronidase(GUS) assay, polymerase...
Ferroptosis is an iron-dependent oxidative, nonapoptotic form of regulated cell death caused by the destruction redox homeostasis. Recent studies have uncovered complex cellular networks that regulate ferroptosis. GINS4 a promoter eukaryotic G 1 /S - cycle as regulator initiation and elongation DNA replication, but little known about its impact on Here, we found was involved in regulation ferroptosis lung adenocarcinoma (LUAD). CRISPR/Cas9-mediated KO facilitated Interestingly, depletion...
Abstract Endometriosis is a common gynecological disorder that causes female infertility. Our recent research found excessive oxidative stress in ovaries of endometriosis patients induced senescence cumulus granulosa cells. Here, we analyzed the transcriptomic and metabolomics profiles follicles mouse model with investigated potential function changed metabolites RNA‐sequencing indicated both lesions mice abnormalities reactive stress, steroid hormone biosynthesis, lipid metabolism. The...