A5053016385- Uterine Myomas and Treatments
- Pregnancy and preeclampsia studies
- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Reproductive Health and Contraception
- Ovarian function and disorders
- Estrogen and related hormone effects
- Gestational Diabetes Research and Management
- Hemoglobinopathies and Related Disorders
- Neonatal Respiratory Health Research
- Protein Kinase Regulation and GTPase Signaling
- Pharmacogenetics and Drug Metabolism
- Enzyme Structure and Function
- Hormonal and reproductive studies
- Neonatal and fetal brain pathology
- Preterm Birth and Chorioamnionitis
- Pluripotent Stem Cells Research
- Inflammatory mediators and NSAID effects
- Photosynthetic Processes and Mechanisms
- Prenatal Screening and Diagnostics
- Gynecological conditions and treatments
- Growth Hormone and Insulin-like Growth Factors
- Reproductive Biology and Fertility
- Metalloenzymes and iron-sulfur proteins
- Protein Structure and Dynamics
University of South Florida
2005-2024
Florida College
1978-2012
Moffitt Cancer Center
2005
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2002
Texas Tech University
2000
Louisiana State University in Shreveport
1998-1999
University Hospital and Clinics
1985-1999
Christie's
1999
AdventHealth Tampa
1998
University of Tampa
1995
A basic, progesterone-induced, intrauterine glycoprotein with a purple coloration can be purified from the uterine fluids of ovariectomized female pigs given daily doses progesterone, by successive chromatography on columns CM-cellulose and Sephadex G-100.The protein contains 1 atom iron per 32,000 molecular weight polypeptide shows phosphatase activity toward p-nitrophenylphosphate as substrate.The pH optimum enzyme is 4.9 apparent K, 2.2 rnM at 30".Activity enhanced 2-to 4-fold in presence...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMoessbauer parameters of putidaredoxin and its selenium analogE. Muenck, P. G. Debrunner, J. C. M. Tsibris, I. GunsalusCite this: Biochemistry 1972, 11, 5, 855–863Publication Date (Print):February 29, 1972Publication History Published online1 May 2002Published inissue 29 February 1972https://pubs.acs.org/doi/10.1021/bi00755a027https://doi.org/10.1021/bi00755a027research-articleACS PublicationsRequest reuse permissionsArticle...
A number of oxidation-reduction proteins, containing iron and an acid-labile form sulfide, have been shown to exhibit low-temperature electron paramagnetic resonance (EPR) signals near g = 1.94, on reduction.l-4 These proteins include oxidases the xanthine type, ferredoxins, nonheme-iron flavoproteins from mitochondria bacteria. By substitution with iron5 sulfur6' 7 isotopes having nonzero nuclear spin, both elements were conclusively participate in center. The magnetic moments may couple...
Preeclampsia (PE) is a common cause of maternal morbidity, characterized by impaired trophoblast invasion and spiral artery transformation resulting in progressive uteroplacental hypoxia. Given the primary role LIN28A LIN28B modulating cell metabolism, differentiation, invasion, we hypothesized that and/or regulates differentiation its dysregulation may contribute to PE. Here show expressed ∼1300-fold higher than human term placenta predominant paralog cultures. The expression mRNA protein...
Protein disulfide isomerase (PDI) is a multifunctional microsomal enzyme that participates in the formation of protein bonds. PDI catalyzes reduction bonds presence excess reduced glutathione and has been implicated reductive degradation insulin; E. coli thioredoxin homologous to two regions can also degrade insulin. activity, measured by 125I-insulin or reactivation randomly oxidized RNase glutathione, non-competitively inhibited estrogens; half-maximal inhibition was observed at...
We have measured insulin and insulin-like growth factor I (IGF-I) binding in human gliomas, meningiomas, normal brain studied the effect of on morphology, proliferation, differentiation central nervous system tumor fetal cells culture. Specific 125I-insulin 125I-IGF-I was demonstrated by competition-inhibition assays. Insulin plasma membrane preparations from 9 freshly isolated 4 glioblastomas multiforme (GBMs), a low-grade glioma, adult brain, brain. IGF-I similar 5 GBMs, Incubations were...
Altered carbohydrate metabolism occurs in women during pregnancy and those using oral contraceptives (OC). Insulin binding to circulating erythrocytes monocytes was studied 77 nonobese, healthy volunteers; they were divided into 4 groups: 1) late pregnant (n = 19),2) OC users taking 50 microgram estrogen daily (OC-50; n 19),3) 35 (OC-35; 18), 4) a control group 21). All nonpregnant volunteers the luteal phase (days 18-21) of menstrual cycle. Oral glucose tolerance tests normal all groups....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies on Donor-Acceptor Complexes Relating to the Intramolecular Association of Riboflavin and Adenosine Moieties Flavin-Adenine Dinucleotide*John C. M. Tsibris, Donald B. McCormick, Lemuel D. WrightCite this: Biochemistry 1965, 4, 3, 504–510Publication Date (Print):March 1, 1965Publication History Published online1 May 2002Published inissue 1 March...
We have previously shown in a typical iron-sulfur protein, putidaredoxinll from Pseudomonas putida, that reduction by one electron equivalent per mole leads to structure which unpaired is shared the two iron atoms present.2This conclusion based on comparison of paramagnetic resonance (EPR) spectrum reduced form natural putidaredoxin with sample isotope Fe56 (I = 0) was replaced Fe57 1/2).The hyperfine splitting due nucleus sufficiently large and well re- solved analysis spectra computer...
During implantation, cytotrophoblasts undergo epithelial-to-mesenchymal transition (EMT) as they differentiate into invasive extravillous trophoblasts (EVTs). The primate-specific microRNA cluster on chromosome 19 (C19MC) is exclusively expressed in the placenta, embryonic stem cells and certain cancers however, its role EMT gene regulation unknown. In situ hybridization for miR-517a/c, a C19MC cistron microRNA, first trimester human placentas displayed strong expression villous gradual...
The oxidation-reduction behavior of the iron sulfide protein, putidaredoxin, isolated from camphor-grown Pseudomonas putida has been examined in native sulfur and selenide-substituted forms.This change replaceable chalcogenide ligand shifts potential at pH 7 -23.5 mv (sulfur)to -245 (selenide).Analysis formal potentials protein over range 6.5 to 10 indicates two proton-linked processes oxidized form (ZPol = 1.0 X 1O-8; K'02 lo-lo) one reduced (K'nl x 10P9).The uersus curves for selenide...
Abstract The binding and function of FMN, 3,6,7-trimethyl-9-(1'-d-ribityl)isoalloxazine 5'-phosphate (3-methyl-FMN),6,7-dichloro-9-(1'-d-ribityl)isoalloxazine (6,7-dichloro-FMN), 6,7-dimethyl-9-(5'-hydroxypentyl)isoalloxazine (5'-hydroxypentyl-FMN) has been investigated with reduced nicotinamide adenine dinucleotide phosphate dehydrogenase NADPH cytochrome c reductase from yeast, NADH oxidase a pseudomonad, pyridoxamine liver, glycolate spinach. Considerable variation observed in the...