A5035118082- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Biomedical Ethics and Regulation
- Organ Transplantation Techniques and Outcomes
- Animal Genetics and Reproduction
- Liver Disease and Transplantation
- Congenital heart defects research
- Nuclear Receptors and Signaling
- Prenatal Screening and Diagnostics
- Virus-based gene therapy research
- Genetic and Kidney Cyst Diseases
- Lipoproteins and Cardiovascular Health
- Mesenchymal stem cell research
- Trace Elements in Health
- Genetics, Aging, and Longevity in Model Organisms
- Ferroptosis and cancer prognosis
- Qualitative Comparative Analysis Research
- Genetics and Neurodevelopmental Disorders
- Inflammatory Biomarkers in Disease Prognosis
- Job Satisfaction and Organizational Behavior
- Advanced Glycation End Products research
- Pharmaceutical Economics and Policy
- Lymphoma Diagnosis and Treatment
West China Hospital of Sichuan University
2015-2024
Zhengzhou University
2024
Sichuan University
2015-2024
University of Hong Kong
2016-2022
Chinese University of Hong Kong
2017-2021
University of Hong Kong - Shenzhen Hospital
2018
Guangzhou Institutes of Biomedicine and Health
2009-2017
Queen Mary Hospital
2016-2017
City University of Hong Kong, Shenzhen Research Institute
2016
First Affiliated Hospital Zhejiang University
2013
Induced pluripotent stem cell (iPS) technology appears to be a general strategy generate cells from any given mammalian species. So far, iPS have been reported for mouse, human, rat, and monkey. These four species also established embryonic (ESC) lines that serve as the gold standard pluripotency comparisons. Attempts made porcine ESC by various means without success. Here we report successful generation of fibroblasts isolated Tibetan miniature pig using modified protocol. The resulting...
The umbilical cord and placenta are extra-embryonic tissues of particular interest for regenerative medicine. They share an early developmental origin a source vast amounts cells with multilineage differentiation potential that poorly immunogenic without controversy. Moreover, these likely exempt from incorporated mutations when compared juvenile or adult donor such as skin fibroblasts keratinocytes. Here we report the efficient generation induced pluripotent stem (iPSCs) mesenchymal matrix...
The recent discovery of induced pluripotent stem cell (iPSC) technology provides an invaluable tool for creating in vitro representations human genetic conditions. This is particularly relevant those diseases that lack adequate animal models or where the species comparison difficult, e.g. imprinting such as neurogenetic disorder Prader-Willi syndrome (PWS). However, reports have unveiled transcriptional and functional differences between iPSCs embryonic cells cases are attributable to...
Many human diseases share a developmental origin that manifests during childhood or maturity. Aneuploid syndromes are caused by supernumerary reduced number of chromosomes and represent an extreme example disease, as they have devastating consequences before after birth. Investigating how alterations in gene dosage drive these conditions is relevant because it might help treat some clinical aspects. It may also provide explanations to quantitative differences expression determine phenotypic...
The ciliary hypothesis for cystic renal diseases postulates that most of these conditions result from abnormalities in the primary cilium, a microtubule-based structure acts as sensor extracellular cues. Inactivation von Hippel-Lindau (VHL) tumor suppressor gene predisposes to cysts and clear cell carcinoma. VHL plays critical role formation cilia kidney epithelium, but underlying mechanisms are poorly understood. Here, we demonstrate inactivation induces HEF1/Cas-L/NEDD9 Aurora kinase A via...
The rise of large language models has opened new avenues for users seeking legal advice. However, often lack professional knowledge, which can lead to questions that omit critical information. This deficiency makes it challenging traditional question-answering systems accurately identify users' actual needs, resulting in imprecise or generalized In this work, we develop a system called Intelligent Legal Assistant, interacts with precisely capture their needs. When user poses question, the...
Wilson's disease (WD) is an autosomal recessive disorder of copper metabolism caused by loss-of-function mutations in ATP7B, which encodes a copper-transporting protein. It characterized excessive deposition tissues, predominantly the liver and brain. We sought to investigate whether gene-corrected patient-specific induced pluripotent stem cell (iPSC)-derived hepatocytes (iHeps) could serve as autologous source for cellular transplantation therapy WD.We first compared vitro phenotype...
Introduction: Gene-edited pigs have become prominent models for studying human disease mechanisms, gene therapy, and xenotransplantation. CRISPR (clustered regularly interspaced short palindromic repeats)/CRISPR-associated 9 (CRISPR/Cas9) technology is a widely employed tool generating gene-edited pigs. Nevertheless, delivering CRISPR/Cas9 to pre-implantation embryos has traditionally posed challenges due its reliance on intricate micromanipulation equipment specialized techniques, resulting...
Recent pre-clinical and clinical studies have suggested that endogenous cardiospheres (eCS) are potentially safe effective for cardiac regeneration following myocardial infarction (MI). Nevertheless the preparation of autologous eCS requires invasive biopsy with limited yield. We describe a novel approach to generate induced (iCS) from adult skin fibroblasts via somatic reprogramming. After infection Sox2, Klf4, Oct4, iCS were generated mouse treated Gsk3β inhibitor-(2'Z,3'E)-...
Highlights•Generation of a comprehensive stem cell model for familial hypercholesterolemia•Generation chimeric mice with engrafted human iPSC-derived iHeps•FH iHeps respond to LDL-C-lowering medications in vitro and vivo•Our can be used preclinical testing novel medicationsSummaryFamilial hypercholesterolemia (FH) causes elevation low-density lipoprotein cholesterol (LDL-C) blood carries an increased risk early-onset cardiovascular disease. A caveat exploration new therapies FH is the lack...
The aim of this study was to evaluate the clinical efficacy postoperative adjuvant transcatheter arterial chemoembolization (TACE) on hepatocellular carcinoma (HCC) with microscopic venous invasion.Data from 76 patients HCC who underwent hepatectomy or without TACE between July 2005 and August 2010 were retrospectively reviewed. Kaplan-Meier method used compare survival groups prognostic factors evaluated by Cox proportional hazard model.The 1-, 3- 5-year disease- free rates 76.3%, 44.5%...
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a structurally endogenous peptide with many biological roles. Maxadilan, 61-amino acid vasodilatory peptide, specifically activates the PACAP type I receptor (PAC1). Although PAC1 has been identified in embryonic stem cells, little known about its presence or effects human induced pluripotent (iPS) cells. In present study, we investigated expression of iPS cells by reverse transcriptase polymerase chain reaction (RT-PCR) and...
Hereditary tyrosinemia type 1 (HT1) is a severe human autosomal recessive disorder caused by the deficiency of fumarylacetoacetate hydroxylase (FAH), an enzyme catalyzing last step in tyrosine degradation pathway. Lack FAH causes accumulation toxic metabolites (fumarylacetoacetate and succinylacetone) blood tissues, ultimately resulting liver kidney damage with onset that ranges from infancy to adolescence. This tissue lethal but can be controlled administration...
Wilson's disease (WD) is an inherited disorder of copper metabolism with predominant hepatic manifestations. Left untreated, it can be fatal. Current therapies focus on treating overload rather than targeting the pathophysiology copper-induced liver injuries. We sought to investigate whether liposome-encapsulated curcumin (LEC) could attenuate underlying WD in a mouse model WD.Subcutaneous administration ATP7B knockout (Atp7b-/-) resulted robust delivery LEC as determined by in-vitro and...
Non-human primate (NHP) models can closely mimic human physiological functions and are therefore highly valuable in biomedical research. Genome editing is now developing rapidly due to the precision efficiency offered by engineered site-specific endonuclease-based systems, such as transcription activator-like effector nucleases (TALENs) clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) system. It has been demonstrated that these...
Familial hypercholesterolemia (FH) is mostly caused by low-density lipoprotein receptor (LDLR) mutations and results in an increased risk of early-onset cardiovascular disease due to marked elevation LDL cholesterol (LDL-C) blood. Statins are the first line lipid-lowering drugs for treating FH other types hypercholesterolemia, but new approaches emerging, particular PCSK9 antibodies, which now being tested clinical trials. To explore novel therapeutic FH, either or formulations, we need...
Ras-related GTP-binding protein A (RagA), encoded by RRAGA gene, initially senses the availability of cellular amino acids (e.g., leucine) and controls translocation mTORC1 to lysosomal membrane. RagA overexpression appears be associated with onset depression. To discover biological roles RagA, we employed CRISPR/Cas9 system generate a gene knockout stem cell line from human induced pluripotent (iPSC) iPSN0003. Such iPSC may help development new therapeutics for