A5100323460- Single-cell and spatial transcriptomics
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Development and Disorders
- Pluripotent Stem Cells Research
- Zebrafish Biomedical Research Applications
- Immune cells in cancer
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- Pancreatic function and diabetes
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- RNA modifications and cancer
- Cell Image Analysis Techniques
- Neuroscience and Neuropharmacology Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Thyroid Cancer Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
- RNA regulation and disease
- Plant Molecular Biology Research
- Retinal and Macular Surgery
- Congenital heart defects research
- 3D Printing in Biomedical Research
- RNA Research and Splicing
- Retinal Diseases and Treatments
- Cancer-related molecular mechanisms research
Center for Excellence in Brain Science and Intelligence Technology
2020-2025
Chinese Academy of Sciences
2013-2025
University of Chinese Academy of Sciences
2017-2025
Beijing Anzhen Hospital
2024-2025
Capital Medical University
2024-2025
Institute of Zoology
2024
State Key Laboratory of Membrane Biology
2024
Suzhou Vocational Health College
2022
BGI Group (China)
2022
New York State Psychiatric Institute
2021
Elucidating the cellular organization of cerebral cortex is critical for understanding brain structure and function. Using large-scale single-nucleus RNA sequencing spatial transcriptomic analysis 143 macaque cortical regions, we obtained a comprehensive atlas 264 transcriptome-defined cell types mapped their distribution across entire cortex. We characterized layer region preferences glutamatergic, GABAergic, non-neuronal types, as well regional differences in cell-type composition...
Induced pluripotent stem cell (iPS cell) holds great potential for applications in regenerative medicine, drug discovery, and disease modeling. We describe here a practical method to generate human iPS cells from urine-derived (UCs) under feeder-free, virus-free, serum-free condition without oncogene c-MYC. showed that this approach could be applied large population with different genetic backgrounds. UCs are easily accessible exhibit high reprogramming efficiency, offering advantages over...
The zebrafish retina grows for a lifetime. Whether embryonic and postembryonic retinogenesis conform to the same developmental program is an outstanding question that remains under debate. Using single-cell RNA sequencing of ∼20,000 cells developing at four different stages, we identified seven distinct states. Each state explicitly expresses gene set. Disruption individual state-specific marker genes results in various defects ranging from small eyes loss retinal cell types. similar...
Abstract Global profile of gene expression at single-cell resolution remains to be determined for primates. Using a recently developed technology (“Stereo-seq”), we have obtained comprehensive spatial transcriptome map the whole-brain level cynomolgus monkeys, with ∼600 genes per cell 10 μm-thick coronal sections (up 15 cm 2 in size). Large-scale single-nucleus RNA-seq analysis ∼1 million cells helped identify types corresponding Stereo-seq profiles, providing 3-D type atlas monkey brain....
Claustrum orchestrates brain functions via its connections with numerous regions, but molecular and cellular organization remains unresolved. Single-nucleus RNA sequencing of 227,750 macaque claustral cells identified 48 transcriptome-defined cell types, most glutamatergic neurons similar to deep-layer insular neurons. Comparison macaque, marmoset, mouse transcriptomes revealed macaque-specific types. Retrograde tracer injections at 67 cortical 7 subcortical regions defined four distinct...
In order to understand the cellular processes that underlie ageing, authors performed plasma proteomics at 10 different ages across lifespan of mouse. They integrated these data with a parallel large study published alongside this paper in same edition (1), which describes 'Mouse Ageing Cell Atlas', single-cell transcriptomic atlas characterizes changes gene expression age 23 tissues. Together, reveal clustered patterns ('trajectory groups') and protein levels, consistent coherent biological...
Altered prefrontal cortex function is implicated in schizophrenia (SCZ) pathophysiology and could arise from imbalance between excitation inhibition (E/I) local circuits. It remains unclear whether how such imbalances relate to genetic etiologies. We used a mouse model of the SCZ-predisposing 22q11.2 deletion (Df(16)A+/− mice) evaluate this lesion affects excitability layer V pyramidal neurons its modulation by dopamine (DA). Df(16)A+/− mice have normal balance E/I at baseline but are unable...
Multidimensional landscapes of regulatory genes in neuronal phenotypes at whole-brain levels the vertebrate remain elusive. We generated single-cell transcriptomes ~67,000 region- and neurotransmitter/neuromodulator-identifiable cells from larval zebrafish brains. Hierarchical clustering based on effector gene profiles (‘terminal features’) distinguished major brain cell types. Sister clusters hierarchical termini displayed similar terminal features. It was further verified by a...
The rapid promotion of single-cell omics in various fields has begun to help solve many problems encountered research, including precision medicine, prenatal diagnosis, and embryo development. Meanwhile, techniques are also constantly updated with increasing demand. For some specific target cells, the workflow from droplet screening sequencing is a preferred option should reduce impact operation steps, such as demulsification cell recovery. We developed an all-in-droplet method integrating...
Abstract Massively-parallel single-cell and single-nucleus RNA sequencing (scRNA-seq, snRNA-seq) requires extensive to achieve proper per-cell coverage, making resources availability of sequencers critical factors for conducting deep transcriptional profiling. CoolMPS is a novel sequencing-by-synthesis approach that relies on nucleotide labeling by re-usable antibodies, but whether it applicable snRNA-seq has not been tested. Here, we use low-cost off-the-shelf protocol chemically convert...
Urine resource cells were collected from a 59-year-old female patient with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS episomal plasmids carrying Oct4, Sox2, Klf4 and miR-302-367. The sustained heterozygous G>T transition mutation on the exon 9 of Men1 gene that was confirmed by sequencing analysis obtained iPSC lines. Karyotyping indicated chromosomes normal appearances numbers. Their pluripotency demonstrated expression, as well their abilities differentiating...
Urine resource cells were collected from a 23-year-old male with multiple endocrine neoplasia type 1 syndrome (MEN1) for generating iPS episomal plasmids. Two stable iPSC lines free of plasmid established. The patient has heterozygous G>T mutation on the exon 9 Men1 gene that was confirmed by sequencing analysis all resulted cell lines. Karyotyping indicated chromosomes normal appearances and numbers. Their pluripotency demonstrated expression their abilities differentiating into three germ...
Correct cell number generation is central to tissue development. However, in vivo roles of coordinated proliferation individual neural progenitors regulating numbers developing tissues and the underlying molecular mechanism remain mostly elusive. Here, we showed that wild-type (WT) donor retinal progenitor cells (RPCs) generated significantly expanded clones host retinae with G1-lengthening by p15 (cdkn2a/b) overexpression (p15+) zebrafish. Further analysis adhesion molecule 3 (cadm3) was...
Abstract Background Hematopoietic stem and progenitor cell (HSPC) subsets in mice have previously been studied using surface markers, more recently single-cell technologies. The recent revolution of analysis is substantially transforming our understanding hematopoiesis, confirming the substantial heterogeneity cells composing hematopoietic system. While dynamic molecular changes at DNA/RNA level underlying hematopoiesis extensively explored, a broad signaling programs landscapes, protein...