A5111401237- Retinal Development and Disorders
- Plant Stress Responses and Tolerance
- Light effects on plants
- Zebrafish Biomedical Research Applications
- Genomics and Phylogenetic Studies
- Mesenchymal stem cell research
- Plant Molecular Biology Research
- Glaucoma and retinal disorders
- Photosynthetic Processes and Mechanisms
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Photoreceptor and optogenetics research
- Retinal Diseases and Treatments
- Microbial Community Ecology and Physiology
- Advanced biosensing and bioanalysis techniques
- Plant and animal studies
- Nematode management and characterization studies
- Aquaculture Nutrition and Growth
- Plant responses to elevated CO2
- Neuroinflammation and Neurodegeneration Mechanisms
- Innovations in Aquaponics and Hydroponics Systems
- Plant nutrient uptake and metabolism
- Aquaculture disease management and microbiota
- Plant-Microbe Interactions and Immunity
- Plant Micronutrient Interactions and Effects
Center for Excellence in Brain Science and Intelligence Technology
2017-2024
University of Chinese Academy of Sciences
2023-2024
Penn Presbyterian Medical Center
2016-2024
Chinese Academy of Sciences
2014-2024
University of Pennsylvania
2016-2024
Zhengzhou University
2008-2024
Huzhou Vocational and Technical College
2021-2024
Sichuan Agricultural University
2022-2024
California University of Pennsylvania
2024
Yangzhou University
2013-2023
A fundamental question in developmental neuroscience is how a collection of progenitor cells proliferates and differentiates to create brain the appropriate size cellular composition. To address this issue, we devised lineage-tracing assays developing zebrafish embryos reconstruct entire retinal lineage progressions vivo thereby provide complete quantitative map generation vertebrate CNS tissue from individual progenitors. These data are consistent with simple model which retina derived set...
Summary Anthocyanins play an important role in the growth of plants, and are beneficial to human health. In MYB‐bHLH‐WD40 (MBW) complex activates genes for anthocyanin biosynthesis. However, rice, WD40 regulators remain be conclusively identified. Here, a crucial biosynthesis gene was fine mapped 43.4‐kb genomic region on chromosome 2, OsTTG1 ( Oryza sativa TRANSPARENT TESTA GLABRA1 ) identified as ideal candidate gene. Subsequently, homozygous mutant osttg1 generated by CRISPR/Cas9 showed...
Elucidating the cellular organization of cerebral cortex is critical for understanding brain structure and function. Using large-scale single-nucleus RNA sequencing spatial transcriptomic analysis 143 macaque cortical regions, we obtained a comprehensive atlas 264 transcriptome-defined cell types mapped their distribution across entire cortex. We characterized layer region preferences glutamatergic, GABAergic, non-neuronal types, as well regional differences in cell-type composition...
cAMP is a key second messenger that regulates diverse cellular functions including neural plasticity. However, the spatiotemporal dynamics of intracellular in intact organisms are largely unknown due to low sensitivity and/or brightness current genetically encoded fluorescent indicators. Here, we report development new circularly permuted GFP (cpGFP)-based indicator G-Flamp1, which exhibits large fluorescence increase (a maximum ΔF/F
Abstract Salt exclusion at the roots and salt secretion in leaves were examined a mangrove, A vicennia officinalis . The non‐secretor mangrove B ruguiera cylindrica was used for comparative study of hydrophobic barrier formation roots. Bypass flow reduced when seedlings previously treated with high concentration. biseriate exodermis detected salt‐treated roots, along an enhanced deposition barriers endodermis. These Na + loading into xylem, accounting 90–95% Prominent found even absence...
Abstract High-temperature (HT) stress is a major environmental that limits plant growth and development. MAPK cascades play key roles in signaling, but their involvement the HT response poorly understood. Here, we describe 47-kD MBP-phosphorylated protein (p47-MBPK) activated tomato (Solanum lycopersicum) leaves under identify it as SlMPK1 by tandem mass spectrometry analysis. Silencing of transgenic plants resulted enhanced tolerance to HT, while overexpression reduced tolerance. Proteomic...
Mesembryanthemum crystallinum is a succulent, facultative CAM plant. Plant growth and photosynthetic characteristics were studied when M. plants grown indoor under LED-lighting with adequate water supply. Plants cultured aeroponically for 16-h photoperiod at an equal photon flux density (PPFD) of 350 µmol m-2 s-1 different red:blue LED ratios: 1) 100:0 (0B); 2) 90:10 (10B); 3) 80:20 (20B), 4) 70:30 (30B), 5) 50:50 (50B) 6)100:0 (100B). 10B condition had the highest shoot root biomass...
Abstract Melatonin is involved in defending against oxidative stress caused by various environmental stresses plants. In this study, the roles of exogenous melatonin regulating local and systemic defense photooxidative cucumber ( Cucumis sativus ) involvement redox signaling were examined. Foliar or rhizospheric treatment with enhanced tolerance to both melatonin‐treated leaves untreated leaves. Increased levels are capable increasing glutathione (reduced [ GSH ]) status. Application H 2 O...
Clonal analysis is helping us understand the dynamics of cell replacement in homeostatic adult tissues (Simons and Clevers, 2011). Such an analysis, however, has not yet been achieved for continuously growing tissues, but essential if we wish to architecture organs. The retinas lower vertebrates grow throughout life, from retinal stem cells (RSCs) progenitor (RPCs) at rim retina, called ciliary marginal zone (CMZ). Here, show that RSCs reside a niche extreme periphery CMZ divide...
Transcriptional regulation of cold-responsive genes is crucial for exogenous melatonin-mediated cold tolerance in plants. Nonetheless, how melatonin regulates largely unknown. In this study, we found that improved watermelon by regulating expression microRNAs (miRNAs). We identified a set miRNAs were regulated under unstressed or conditions. Importantly, mRNA-seq analysis revealed melatonin-induced downregulation some miRNAs, such as miR159-5p, miR858, miR8029-3p, and novel-m0048-3p...
The zebrafish retina grows for a lifetime. Whether embryonic and postembryonic retinogenesis conform to the same developmental program is an outstanding question that remains under debate. Using single-cell RNA sequencing of ∼20,000 cells developing at four different stages, we identified seven distinct states. Each state explicitly expresses gene set. Disruption individual state-specific marker genes results in various defects ranging from small eyes loss retinal cell types. similar...
Autosomal recessive primary microcephaly (MCPH) is a congenital disorder characterized by significantly reduced brain size and mental retardation. Nine genes are currently known to be associated with the condition, all of which encode centrosomal or spindle pole proteins. MCPH reduction in proliferation neural progenitors during fetal development. The cellular mechanisms underlying defect, however, not fully understood. zebrafish retinal neuroepithelium provides an ideal system investigate...
The ability to image cells live and in situ as they proliferate differentiate has proved be an invaluable asset biologists investigating developmental processes. Here, we describe a Spectrum of Fates approach that allows the identification all major neuronal subtypes zebrafish retina simultaneously. is based on combinatorial expression differently coloured fluorescent proteins driven by promoters transcription factors are expressed overlapping subsets retinal neurons. show how can used...
Glaucoma is a group of progressive optic neuropathies that share common biological and clinical characteristics including irreversible changes to the nerve visual field loss caused by death retinal ganglion cells (RGCs). The RGCs manifests as characteristic cupping or degeneration, resulting in patients with Glaucoma. Published studies on vitro RGC differentiation from stem utilized classical signaling pathways mimicking development vivo. Although many strategies allowed for generation RGCs,...
It is of great significance for the aquaculture industry to determine how rearing salinity impacts fish flesh quality. In present study, largemouth bass was cultured in different salinities (0%, 0.3%, 0.9%) 10 weeks, and effect on texture, flavor compounds, taste, fatty acid composition evaluated. We show that not only increased water-holding capacity, but also enhanced muscle hardness, chewiness, gumminess, adhesiveness, which consistent with finding shear value test. Morphology analysis...
Loss of neurons in the neural retina is a leading cause vision loss. While humans do not possess capacity for retinal regeneration, zebrafish can achieve this through activation resident Müller glia. Remarkably, despite presence glia and other mammalian vertebrates, these cells lack an intrinsic ability to contribute regeneration. Upon activation, adopt stem cell-like state, undergo proliferation generate new neurons. However, underlying molecular mechanisms subsequent regeneration remains...
Respiratory burst oxidase homologs (RBOHs) are important proteins that catalyze the production of reactive oxygen species (ROS), which play roles in growth and stress response. For a comprehensive analysis SmRBOH genes, we conducted genome-wide identification gene family eggplant (Solanum melongena L.) analyzed expression SmRBOHs under abiotic (salt, high-temperature, low-temperature) biotic (Verticillium dahliae inoculation) by quantitative real-time PCR (qRT-PCR). The result showed total...
The molecular and cellular organization of the primate cerebellum remains poorly characterized. We obtained single-cell spatial transcriptomic atlases macaque, marmoset, mouse cerebella identified primate-specific cell subtypes, including Purkinje cells molecular-layer interneurons, that show different expression glutamate ionotropic receptor Delta type subunit 2 ( GRID2 ) gene. Distinct gene profiles were found in anterior, posterior, vestibular regions all species, whereas region-selective...
In lower vertebrates, retinal Müller glia (MG) exhibit a life-long capacity of cell-cycle re-entry to regenerate neurons following the injury. However, mechanism driving such injury-induced MG remains incompletely understood. Combining single-cell transcriptomic analysis and in-vivo clonal analysis, we identified previously undescribed cxcl18b -defined transitional states as essential routes towards proliferation green/red cone (G/R cone) ablation. Microglial inflammation was necessary for...