Neal S. Peachey
A5024900490- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Retinal Diseases and Treatments
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Retinal Imaging and Analysis
- Glaucoma and retinal disorders
- Connexins and lens biology
- Receptor Mechanisms and Signaling
- Cholesterol and Lipid Metabolism
- Visual perception and processing mechanisms
- Neural dynamics and brain function
- bioluminescence and chemiluminescence research
- Retinal and Macular Surgery
- Peroxisome Proliferator-Activated Receptors
- Neurobiology and Insect Physiology Research
- Cellular transport and secretion
- Circadian rhythm and melatonin
- Ocular and Laser Science Research
- Retinoids in leukemia and cellular processes
- Ocular Oncology and Treatments
- Retinopathy of Prematurity Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Lipid metabolism and disorders
- Complement system in diseases
Cleveland Eye Clinic
2016-2025
Cleveland Clinic
2016-2025
Cleveland Clinic Lerner College of Medicine
2015-2025
Case Western Reserve University
2015-2025
VA Northeast Ohio Healthcare System
2021-2025
Louis Stokes Cleveland VA Medical Center
2014-2024
Providence VA Medical Center
2023
Creative Commons
2018
Ophthalmology Associates (United States)
2001-2017
University Hospitals of Cleveland
2014
We executed a genome-wide association scan for age-related macular degeneration (AMD) in 2,157 cases and 1,150 controls. Our results validate AMD susceptibility loci near CFH ( P < 10 −75 ), ARMS2 −59 C2/CFB −20 C3 −9 CFI −6 ). compared our top findings with the Tufts/Massachusetts General Hospital study of advanced (821 cases, 1,709 controls) genotyped 30 promising markers additional individuals (up to 7,749 4,625 controls). With these data, we identified locus TIMP3 (overall = 1.1 × −11...
Although biochemical experiments suggest that rhodopsin and other receptors coupled to heterotrimeric guanosine triphosphate-binding proteins (G proteins) are inactivated by phosphorylation near the carboxyl (COOH)-terminus subsequent binding of a capping protein, little is known about quenching process in vivo. Flash responses were recorded from rods transgenic mice which fraction molecules lacked COOH-terminal sites. In single photon regime, abnormally prolonged responses, attributed...
On bipolar cells are connected to photoreceptors via a sign-inverting synapse. At this synapse, glutamate binds metabotropic receptor which couples the closure of cation-selective transduction channel. The molecular identity both and G protein known, but channel has remained elusive. Here, we show that in mouse rod cells, subtype cell, is likely be member TRP family channels. To evoke current, antagonist LY341495 was applied dendrites were bathed solution containing mGluR6 agonists l -AP4 or...
Glutamate release from photoreceptor terminals is controlled by voltage-dependent calcium channels (VDCCs). In humans, mutations in the Cacna1f gene, encoding α 1F subunit of VDCCs, underlie incomplete form X-linked congenital stationary night blindness (CSNB2). These impair synaptic transmission rod and cone photoreceptors to bipolar cells. Here, we report anatomical functional characterizations retina nob2 ( no b-wave 2 ) mouse, a naturally occurring mutant caused null mutation . Not...
Mutations in VMD2, encoding bestrophin (best-1), cause Best vitelliform macular dystrophy (BMD), adult-onset (AVMD), and autosomal dominant vitreoretinochoroidopathy (ADVIRC). BMD is distinguished from AVMD by a diminished electrooculogram light peak (LP) the absence of changes flash electroretinogram. Although LP thought to be generated best-1, we find enhanced luminance responsiveness with normal amplitude Vmd2−/− mice no differences cellular Cl− currents comparison Vmd2+/+ littermates....
Mutations in the VMD2 gene cause Best's disease, an inherited form of macular degeneration. The reduction light-peak amplitude patient's electro-oculogram suggests that bestrophin-1 influences membrane conductance retinal pigment epithelium (RPE). Systemic application L-type Ca2+ channel blocker nimodipine reduced rat electroretinogram but not a- and b-waves. Expression a RPE cell line (RPE-J) led to changes properties. Wild-type induced acceleration activation kinetics Ba2+ currents through...
Neuronal pentraxins (NPs) define a family of proteins that are homologous to C-reactive and acute-phase in the immune system have been hypothesized be involved activity-dependent synaptic plasticity. To investigate role NPs <i>in vivo</i>, we generated mice lack one, two, or all three NPs. NP1/2 knock-out exhibited defects segregation eye-specific retinal ganglion cell (RGC) projections dorsal lateral geniculate nucleus, process involves synapse formation elimination. Retinas from lacking...
We compared psychophysical and electroretinographic test results of four patients with X-linked juvenile retinoschisis who had clinically apparent lesions isolated to the foveal area. The b-wave flash electroretinogram was selectively reduced, while a-wave within normal range. Oscillatory potentials generated by either rod or cone systems were markedly reduced. Absolute thresholds outside fovea only moderately elevated, indicating that sensory neural pathways were, large, operating limited...
The retina is one of the most metabolically active tissues in body and utilizes glucose to produce energy intermediates required for daily renewal photoreceptor cell outer segments. Glucose transporter 1 (GLUT1) facilitates transport across blood retinal barrier (BRB) formed by pigment epithelium (RPE) inner BRB endothelium. We used conditional knockout mice study impact reducing RPE on Müller glial cells. Transgenic expressing Cre recombinase under control Bestrophin1 ( Best1) promoter were...
Direct reprogramming of glia into neurons is a potentially promising approach for the replacement lost to injury or neurodegenerative disorders. Knockdown polypyrimidine tract-binding protein Ptbp1 has been recently reported induce efficient conversion retinal Mϋller functional neurons. Here, we use combination genetic lineage tracing, single-cell RNA sequencing (scRNA-seq), and electroretinogram analysis show that selective induction either heterozygous homozygous loss-of-function mutants...
There are currently no therapies to restore vision patients blinded by photoreceptor degeneration. This project concerns an experimental approach toward a semiconductor-based subretinal prosthetic designed electrically stimulate the retina. The present study describes surgical techniques for implanting silicon microphotodiode array in cat space and subsequent studies of implant biocompatibility durability. Using single-port vitreoretinal approach, implants were placed into right eye normal...
The etiology of primary open angle glaucoma, a leading cause age-related blindness, remains poorly defined, although elevated intraocular pressure (IOP) contributes to the disease progression. To better understand mechanisms causing IOP from aqueous humor circulation, we pursued proteomic analyses trabecular meshwork (TM) glaucoma and age-matched control donors. These demonstrated that Cochlin, protein associated with deafness disorder DFNA9, is present in glaucomatous but absent normal TM....