A5070205434- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Photoreceptor and optogenetics research
- Cancer, Hypoxia, and Metabolism
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Cellular transport and secretion
- Lymphoma Diagnosis and Treatment
- Amino Acid Enzymes and Metabolism
- Connexins and lens biology
- Cytomegalovirus and herpesvirus research
- Zebrafish Biomedical Research Applications
- RNA and protein synthesis mechanisms
- AI in cancer detection
- Peroxisome Proliferator-Activated Receptors
- Click Chemistry and Applications
- Radiomics and Machine Learning in Medical Imaging
- 3D Printing in Biomedical Research
- Ovarian cancer diagnosis and treatment
- Chronic Lymphocytic Leukemia Research
- interferon and immune responses
- Ocular Diseases and Behçet’s Syndrome
- Global Cancer Incidence and Screening
- Redox biology and oxidative stress
- Monoclonal and Polyclonal Antibodies Research
University of Michigan–Ann Arbor
2024-2025
Sidney Kimmel Cancer Center
2024
Thomas Jefferson University
2015-2024
W.K. Kellogg Foundation
2024
City Of Hope National Medical Center
2024
Institut de la Vision
2022
Inserm
2022
Sorbonne Université
2022
Centre National de la Recherche Scientifique
2022
James Madison University
2017
The retina is one of the most metabolically active tissues in body and utilizes glucose to produce energy intermediates required for daily renewal photoreceptor cell outer segments. Glucose transporter 1 (GLUT1) facilitates transport across blood retinal barrier (BRB) formed by pigment epithelium (RPE) inner BRB endothelium. We used conditional knockout mice study impact reducing RPE on Müller glial cells. Transgenic expressing Cre recombinase under control Bestrophin1 ( Best1) promoter were...
Photoreceptors consume glucose supplied by the choriocapillaris to support phototransduction and outer segment (OS) renewal. Reduced supply underlies photoreceptor cell death in inherited retinal degeneration age-related disease. We have previously shown that restricting transport into retina conditional deletion of Slc2a1 encoding GLUT1 resulted loss impaired OS However, neurons, glia, pigment epithelium play specialized, synergistic roles metabolite exchange, cell-specific map uptake...
Photoreceptors in the retina of a vertebrate’s eye are supported by tissue adjacent to retina, retinal pigment epithelium (RPE). The RPE delivers glucose outer consumes photoreceptor segments discs, and regenerates 11-cis-retinal. Here we address question whether photoreceptors also provide metabolic support RPE. We use complementary approaches animal models show that is primary fuel for cell type consume glucose, lactate derived from consumption transported catabolized These data rigorously...
Photoreceptors in the retina of a vertebrate’s eye are supported by tissue adjacent to retina, retinal pigment epithelium (RPE). The RPE delivers glucose outer consumes photoreceptor segments discs, and regenerates 11-cis-retinal. Here we address question whether photoreceptors also provide metabolic support RPE. We use complementary approaches animal models show that is primary fuel for cell type consume glucose, lactate derived from consumption transported catabolized These data rigorously...
The neural retina metabolizes glucose through aerobic glycolysis generating large amounts of lactate. Lactate flux into and out cells is regulated by proton-coupled monocarboxylate transporters (MCTs), which are encoded members the Slc16a family. MCT1, MCT3, MCT4 expressed in require association with accessory protein basigin, Bsg, for maturation trafficking to plasma membrane. Bsg-/- mice have severely reduced electroretinograms (ERGs) progressive photoreceptor degeneration, presumed be...
The outer segment (OS) organelle of vertebrate photoreceptors is a highly specialized cilium evolved to capture light and initiate response. plasma membrane which envelopes the OS plays vital diverse roles in supporting photoreceptor function health. However, little known about identity its protein constituents, as this cannot be purified homogeneity. In study, we used technique correlation profiling identify unique proteins. To achieve this, label-free quantitative MS compare relative...
Mitochondrial metabolism is critical for the normal function of retinal pigment epithelium (RPE), a monolayer cells in retina important photoreceptor survival. RPE mitochondrial dysfunction hallmark age-related macular degeneration (AMD), leading cause irreversible blindness developed world, and proliferative vitreoretinopathy (PVR), blinding complication detachments. degenerative conditions have been well-modeled by culture systems that are highly differentiated polarized to mimic vivo RPE....
Abstract The retinal pigment epithelium (RPE) is omnivorous and can utilize a wide range of substrates for oxidative phosphorylation. Certain tissues with high mitochondrial metabolic load are capable ketogenesis, biochemical pathway that consolidates acetyl-CoA into ketone bodies. Earlier work demonstrated the RPE expresses rate-limiting enzyme 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), indeed produces bodies, including beta-hydroxybutyrate (β-HB). Prior work, based on detecting...
Abstract This study evaluates the reliability of largest public-facing large language models in providing accurate breast cancer radiotherapy recommendations. We assessed ChatGPT 3.5, 4, 4o, Claude 3.5 Sonnet, and o1 three common clinical scenarios. The cases are as follows: post-lumpectomy a 40 year old woman, (2) postmastectomy radiation woman with 4+ lymph nodes, (3) an 80 early stage tumor negative axillary dissection. Each case was designed to be unambiguous respect Level I evidence...
Human cytomegalovirus (CMV) causes a common congenital infection leading to long-term neurological impairments including brain, cochlear, and ocular pathology. Infection of newborn mice with murine (M)CMV is an established model neuropathology caused by CMV infection, recent work suggesting that brain pathology may be driven immune responses. In the eye, however, retinitis thought result from virus-driven necrosis in absence T cell We found MCMV recapitulates human eye disease after focal...
SUMMARY The nucleoredoxin-like genes NXNL1 and NXNL2 were identified through the biological activity of rod-derived cone viability factors (RdCVF RdCVF2), alternatively spliced variants produced by intron retention, that mediate signaling between rod photoreceptors stimulating glucose uptake. These therapeutic for inherited retinal degenerations also produce splicing thioredoxin-like proteins reduce oxidized cysteines in photoreceptor proteins. first NXNL date from animal phyla. Intron...
Abstract Locally advanced prostate cancers (LAPC) are aggressive, have poor prognosis, and associated with high recurrence rates conversion to castrate resistance. The molecular underpinnings of LAPC being investigated identify novel therapeutics. Animal models shown that caloric restriction (CR) can potentially prevent the initiation cancer. We propose CR may be used as a therapeutic intervention enhance outcomes radiation treatment by altering profile tumors. To assess effect in vivo,...