A5086356122- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Cellular transport and secretion
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Methane Hydrates and Related Phenomena
- Drug Transport and Resistance Mechanisms
- Receptor Mechanisms and Signaling
- ATP Synthase and ATPases Research
- Connexins and lens biology
- Toxin Mechanisms and Immunotoxins
- Cholesterol and Lipid Metabolism
- Neuroscience and Neuropharmacology Research
- bioluminescence and chemiluminescence research
- Glycosylation and Glycoproteins Research
- Retinoids in leukemia and cellular processes
- Neurobiology and Insect Physiology Research
- Monoclonal and Polyclonal Antibodies Research
- Photochromic and Fluorescence Chemistry
- Erythrocyte Function and Pathophysiology
- Ion Transport and Channel Regulation
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- RNA regulation and disease
- Retinal and Macular Surgery
University of British Columbia
2016-2025
Okanagan University College
2019
Coquitlam College
2016
Health Sciences Centre
2011
Johns Hopkins University
1994-2010
University of Würzburg
2002
University of Birmingham
2002
Johns Hopkins Medicine
1994-2002
Forschungszentrum Jülich
1997
Harvard University
1996
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPrimary structure effects on peptide group hydrogen exchangeR. S. Molday, W. Englander, and R. G. KallenCite this: Biochemistry 1972, 11, 2, 150–158Publication Date (Print):January 18, 1972Publication History Published online1 May 2002Published inissue 18 January 1972https://pubs.acs.org/doi/10.1021/bi00752a003https://doi.org/10.1021/bi00752a003research-articleACS PublicationsRequest reuse permissionsArticle Views1331Altmetric-Citations338LEARN...
We report here the high-level expression of a synthetic gene for bovine rhodopsin in transfected monkey kidney COS-1 cells. Rhodopsin is produced these cells to level 0.3% cell protein, and it binds exogenously added 11-cis-retinal generate characteristic absorption spectrum. describe one-step immunoaffinity procedure purification essentially homogeneity. The activates GTPase activity transducin light-dependent manner with same specific as that purified rhodopsin. Electron microscopy...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMonoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probesR. S. Molday D. MacKenzieCite this: Biochemistry 1983, 22, 3, 653–660Publication Date (Print):February 1, 1983Publication History Published online1 May 2002Published inissue 1 February 1983https://pubs.acs.org/doi/10.1021/bi00272a020https://doi.org/10.1021/bi00272a020research-articleACS PublicationsRequest reuse permissionsArticle...
ATP-binding cassette (ABC) transporters comprise a superfamily of proteins, which actively transport variety compounds across cell membranes. Mammalian and most eukaryotic ABC function as exporters, flipping or extruding substrates from the cytoplasmic to extracellular lumen side Prokaryotic either exporters importers. Here we show that ABCA4, an transporter found in retinal photoreceptor cells associated with Stargardt macular degeneration, is novel importer flips...
Many substrates for P-glycoprotein, an ABC transporter that mediates multidrug resistance in mammalian cells, have been shown to stimulate its ATPase activity vitro. In the present study, we used this property as a criterion search natural and artificial and/or allosteric regulators of ABCR, rod photoreceptor-specific responsible Stargardt disease, early onset macular degeneration. ABCR was immunoaffinity purified apparent homogeneity from bovine outer segments reconstituted into liposomes....
Outer segments of mammalian rod photoreceptor cells contain an abundantly expressed membrane protein that migrates with apparent molecular mass 220 kDa by SDS-gel electrophoresis. We have purified the bovine immunoaffinity chromatography, determined its primary structure cDNA cloning and direct peptide sequence analysis, mapped distribution in photoreceptors immunocytochemical biochemical methods. The full-length encodes a 2280-amino acid (calculated 257 kDa) consisting two structurally...
The outer segments of vertebrate rod photoreceptor cells consist an ordered stack membrane disks, which, except for a few nascent disks at the base segment, is surrounded by separate plasma membrane. Previous studies indicate that protein, peripherin or peripherin/rds, localized along rim mature segments. A mutation in gene this protein has been reported to be responsible retinal degeneration rds mouse. In present study, we have shown immunogold labeling rat and ground squirrel retinas...
Retinal degeneration slow (rds) is a retinal disorder of an inbred strain mice in which the outer segment photoreceptor cell fails to develop. A candidate gene has recently been described for rds defect [Travis, G. H., Brennan, M. B., Danielson, P. E., Kozak, C. & Sutcliffe, J. (1989) Nature (London) 338, 70-73]. Neither identity normal product nor its intracellular localization had determined. We report here that amino acid sequence bovine photoreceptor-cell protein peripherin, was...
New immunochemical reagents consisting of antibodies bound to small latex spheres were used as visual markers for the detection and localization cell surface antigens by scanning electron microscopy. Cross-linked various sizes from 300 3,4000 A in diameter synthesized aqueous emulsion copolymerization methacrylate derivatives containing hydroxyl carboxyl functional groups. Proteins other molecules primary amino groups covalently bonded acrylic under a variety mild conditions carbodiimide,...
Deleterious mutations in RS1 encoding retinoschisin are associated with X-linked juvenile retinoschisis (RS), a common form of macular degeneration males. The disorder is characterized by negative electroretinogram pattern and splitting the inner retina. To gain further insight into function protein its role cellular pathology RS, we have generated knockout mice deficient Rs1h , murine ortholog human gene. We show that pathologic changes hemizygous −/Y male evenly distributed across retina,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLocalization of binding sites for carboxyl terminal specific anti-rhodopsin monoclonal antibodies using synthetic peptidesDonald MacKenzie, Anatol Arendt, Paul Hargrave, J. Hugh McDowell, and Robert S. MoldayCite this: Biochemistry 1984, 23, 26, 6544–6549Publication Date (Print):December 18, 1984Publication History Published online1 May 2002Published inissue 18 December...
The rod cGMP-gated channel is localized in the plasma membrane of photoreceptor outer segments, where it plays a central role phototransduction. It consists α- and β-subunits that assemble into heterotetrameric protein. Each subunit contains structural features characteristic nucleotide-gated channels, including cGMP-binding domain, multiple membrane-spanning pore region. In addition, β-subunit has large glutamic acid- proline-rich region called GARP also expressed as two soluble protein...