A5033915515- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Retinal and Optic Conditions
- Glaucoma and retinal disorders
- Photoreceptor and optogenetics research
- Ocular Diseases and Behçet’s Syndrome
- Photoacoustic and Ultrasonic Imaging
- Optical Coherence Tomography Applications
- Connexins and lens biology
- Retinoids in leukemia and cellular processes
- Laser Applications in Dentistry and Medicine
- Ocular and Laser Science Research
- Systemic Lupus Erythematosus Research
- Neuroscience and Neural Engineering
- Reproductive tract infections research
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Imaging and Analysis
- Advanced Glycation End Products research
- Complement system in diseases
- Neuroscience and Neuropharmacology Research
- Cancer, Hypoxia, and Metabolism
- Heat shock proteins research
- Retinopathy of Prematurity Studies
- Optical Imaging and Spectroscopy Techniques
- Infrared Thermography in Medicine
Penn Presbyterian Medical Center
2018-2025
University of Pennsylvania
2018-2025
California University of Pennsylvania
2023-2024
Cleveland Clinic
2013-2023
Cleveland Eye Clinic
2013-2023
Cleveland Clinic Lerner College of Medicine
2010-2019
The University of Texas Medical Branch at Galveston
2003-2013
Institut de la Vision
2013
Texas Medical Center
2013
Ophthalmology Associates (United States)
2012
The retina is one of the most metabolically active tissues in body and utilizes glucose to produce energy intermediates required for daily renewal photoreceptor cell outer segments. Glucose transporter 1 (GLUT1) facilitates transport across blood retinal barrier (BRB) formed by pigment epithelium (RPE) inner BRB endothelium. We used conditional knockout mice study impact reducing RPE on Müller glial cells. Transgenic expressing Cre recombinase under control Bestrophin1 ( Best1) promoter were...
In the diabetic retina, cellular changes in retinal pigment epithelium (RPE) and neurons occur before vision loss or retinopathy can be identified clinically. The precise etiologies of pathology are poorly defined, it remains unclear if onset progression dysfunction differ between type 1 2 diabetes. Three mouse models were used to compare time course RPE involvement C57BL/6J mice injected with streptozotocin (STZ mice) modeled diabetes, whereas Lepr db/db on both BKS B6.BKS background...
High levels of oxidative radicals generated by daily light exposure and high metabolic rate suggest that the antioxidant machinery retina retinal pigment epithelium (RPE) is crucial for their survival. DJ-1 a redox-sensitive protein has been shown to have neuroprotective function in brain Parkinson's disease other neurodegenerative diseases. Here, we analyzed role during stress aging. We induced low-level young (3-month-old) old (15-month-old) C57BL/6J (WT) knockout (KO) mice evaluated...
Oxidative stress has been implicated in the pathogenesis of age-related macular degeneration, leading cause blindness older adults, with retinal pigment epithelium (RPE) cells playing a key role. To better understand cytotoxic mechanisms underlying oxidative stress, we used cell culture and mouse models iron overload, as can catalyze reactive oxygen species formation RPE. Iron-loading cultured induced pluripotent stem cell-derived RPE increased lysosomal abundance, impaired proteolysis...
Abstract Lipid processing by the retinal pigment epithelium (RPE) is necessary to maintain health and function. Dysregulation of lipid homeostasis due normal aging or age‐related disease triggers accumulation within RPE, on Bruch's membrane (BrM), in subretinal space. In its role as a hub for trafficking into out neural retina, RPE packages significant amount droplets storage apolipoprotein B (APOB)‐containing lipoproteins (Blps) export. Microsomal triglyceride transfer protein (MTP),...
Malignant pleural mesothelioma often recurs locally in spite of aggressive resection by extrapleural pneumonectomy and conventional radiotherapy. This may be due to failure recognize the extent clinical target volume (CTV) or suboptimal dose delivery a that abuts heart, esophagus, liver, lung, kidney, spinal cord. We report how these geometric/dosimetric constraints were overcome exploiting intensity-modulated radiotherapy first cohort patient.Twenty-eight patients who had undergone treated...
Abstract Iron has been implicated in the pathogenesis of age‐related retinal diseases, including macular degeneration (AMD). Previous work showed that intravitreal (IVT) injection iron induces acute photoreceptor death, lipid peroxidation, and autofluorescence (AF). Herein, we extend this work, finding surprising chronic features model: geographic atrophy sympathetic ophthalmia. We provide new mechanistic insights derived from focal AF photoreceptors, quantification bisretinoids,...
Oxidative stress plays a central role in age-related macular degeneration (AMD). Iron, potent generator of hydroxyl radicals through the Fenton reaction, has been implicated AMD. One easily oxidized molecule is docosahexaenoic acid (DHA), most abundant polyunsaturated fatty photoreceptor membranes. Oxidation DHA produces toxic oxidation products including carboxyethylpyrrole (CEP) adducts, which are increased retinas AMD patients. In this study, we hypothesized that deuterium substitution on...
Photoreceptors consume glucose supplied by the choriocapillaris to support phototransduction and outer segment (OS) renewal. Reduced supply underlies photoreceptor cell death in inherited retinal degeneration age-related disease. We have previously shown that restricting transport into retina conditional deletion of Slc2a1 encoding GLUT1 resulted loss impaired OS However, neurons, glia, pigment epithelium play specialized, synergistic roles metabolite exchange, cell-specific map uptake...
Purpose: Geographic atrophy (GA), an advanced form of dry age-related macular degeneration (AMD), has limited treatment options. This study introduces a novel mouse model featuring expanding GA patch that can be used to test mechanisms and therapeutics. Methods: C57Bl/6J male mice (n = 96) aged 9–10 weeks received intraperitoneal (IP) injection 20 mg/kg sodium iodate (NaIO3). In vivo confocal scanning laser ophthalmoscope (cSLO) optical coherence tomography imaging were done at one, four,...
Optoacoustic (OA) tomography has demonstrated utility in identifying blood-rich malignancies breast tissue. We describe the development and characterization of a laser OA imaging system for prostate (LOIS-P). The consists fiber-coupled Q-switched operating at 757 nm, commercial 128-channel ultrasonic probe, digital signal processor, software that uses filtered radial back-projection algorithm image reconstruction. is used to reconstruct images lesion induced vivo canine prostate. obtained...
Like other neurons, retinal cells utilize autophagic pathways to maintain cell homeostasis. The mammalian retina relies on heterophagy and selective autophagy efficiently degrade metabolize ingested lipids with disruption in associated degradation contributing age related disorders. pigment epithelium (RPE) supports photoreceptor renewal by daily phagocytosis of shed outer segments (OS). ingestion these lipid-rich OS imposes a constant degradative burden terminally differentiated cells....
Highlights•Cx46-mediated cell-cell communication is essential for GBM self-renewal•A communication-based screening platform identifies clofazimine as a Cx46 inhibitor•Clofazimine targets CSCs and has little effect on non-stem tumor cells•Clofazimine treatment of tumor-bearing mice extends survival in vivoSummaryGap-junction-mediated enables cells to synchronize complex processes. We previously found that glioblastoma cancer stem (CSCs) express higher levels the gap junction protein compared...
Background and Objectives Temporary dermal clearing, i.e., reduction in the attenuation coefficient of dermis epidermis, may lead to improved laser tattoo removal by providing increased efficiency delivery embedded ink particles enabling use shorter wavelength visible lasers more effective on certain inks. Study Designs/Materials Methods In a hairless guinea pig model human tattoo, we tested both intradermal transdermal application glycerol, using visual inspection, spectral analysis,...
Six patients with cerebral arteriovenous malformations which did not show any pathological circulation at angiography are described. Computed tomogram appearances of such lesions may be difficult to distinguish from tumours. The need for surgical exploration in localised high attenuation uncertain nature is stressed, and the literature reviewed.
In Brief Background: Access to readily available large animal models and sensitive noninvasive techniques that can be used for the evaluation of microbicide-induced changes in tissue could significantly facilitate preclinical evaluations microbicide safety. The sheep cervicovaginal tract, with stratified squamous epithelium similar humans, holds promise as a model before nonhuman primates. addition, optical coherence tomography (OCT) enable high resolution visualization morphology assessment...
The neural retina metabolizes glucose through aerobic glycolysis generating large amounts of lactate. Lactate flux into and out cells is regulated by proton-coupled monocarboxylate transporters (MCTs), which are encoded members the Slc16a family. MCT1, MCT3, MCT4 expressed in require association with accessory protein basigin, Bsg, for maturation trafficking to plasma membrane. Bsg-/- mice have severely reduced electroretinograms (ERGs) progressive photoreceptor degeneration, presumed be...
To describe the pathology of AMD in eyes with geographic atrophy (GA) using confocal scanning laser ophthalmoscopy (SLO) blue light autofluorescence (BAF), and near-infrared (IR) AF to correlate it histology immunohistochemistry analysis at margins GA lesion.Enucleated, fixed from seventeen donors were imaged analyzed by BAF-SLO, IRAF-SLO, fundus macroscopy (FM). Tissue lesions was cut processed for resin embedding or cryosectioning fluorescence green far-red channels, assess markers...
Abstract Recent studies have suggested that reagents inhibiting complement activation could be effective in treating T cell mediated autoimmune diseases such as uveitis. However, the precise role of anaphylatoxin receptors (C3a and C5a receptors) pathogenesis uveitis remains elusive controversial. We induced experimental mice deficient or sufficient both C3a rigorously compared their retinal phenotype using various imaging techniques, including indirect ophthalmoscopy, confocal scanning...