A5044721613- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Blood disorders and treatments
- Platelet Disorders and Treatments
- T-cell and B-cell Immunology
- Bacterial biofilms and quorum sensing
- Antimicrobial Peptides and Activities
- Immune Cell Function and Interaction
- Urinary Bladder and Prostate Research
- Insect and Arachnid Ecology and Behavior
- Nuclear Receptors and Signaling
- Antimicrobial Resistance in Staphylococcus
- Plant and animal studies
- Immunodeficiency and Autoimmune Disorders
- Calcium signaling and nucleotide metabolism
- Spinal Dysraphism and Malformations
- Blood groups and transfusion
- CRISPR and Genetic Engineering
- Biochemical and Structural Characterization
- Autophagy in Disease and Therapy
- Parkinson's Disease Mechanisms and Treatments
- Retinal Diseases and Treatments
- Erythrocyte Function and Pathophysiology
- Insect-Plant Interactions and Control
- Blood properties and coagulation
- Urological Disorders and Treatments
Children's Hospital of Philadelphia
2023-2024
Treatment Research Institute
2024
University of Pennsylvania
2019
Oxidative stress has been implicated in the pathogenesis of age-related macular degeneration, leading cause blindness older adults, with retinal pigment epithelium (RPE) cells playing a key role. To better understand cytotoxic mechanisms underlying oxidative stress, we used cell culture and mouse models iron overload, as can catalyze reactive oxygen species formation RPE. Iron-loading cultured induced pluripotent stem cell-derived RPE increased lysosomal abundance, impaired proteolysis...
Alpha-synuclein (αSyn) protein levels correlate with the risk and severity of Parkinson’s disease related neurodegenerative diseases. Lowering αSyn is being actively investigated as a therapeutic modality. Here, we systematically map regulatory network that controls endogenous using sequential CRISPR-knockout -interference screens in an gene ( SNCA )–tagged cell line induced pluripotent stem cell–derived neurons (iNeurons). We uncover modifiers at multiple layers, amino-terminal...
The E3 ubiquitin ligase Itch regulates antibody levels and prevents autoimmune disease in humans mice, yet how B cell fate or function is unknown. We now show that directly limits activity. While Itch-deficient mice displayed normal numbers of preimmune populations, they showed elevated antigen-experienced cells. Mixed bone marrow chimeras revealed acts within cells to limit naive and, a greater extent, germinal center (GC) numbers. lacking exhibited increased proliferation, glycolytic...
Abstract During infection, virus-specific CD8 + T cells undergo rapid bursts of proliferation and differentiate into effector that kill virus-infected reduce viral load. This clonal expansion can put at significant risk for replication-induced DNA damage. Here, we find c-Myc links cell to damage response pathways though the E3 ubiquitin ligase, Cullin 4b (Cul4b). Following activation, increases levels Cul4b other members RING Ligase 4 (CRL4) complex. Despite expressing high levels,...
Abstract Background and Objective Recent clinical studies have shown that transfusions of adult platelets increase morbidity mortality in preterm infants. Neonatal are hyporesponsive to agonist stimulation, emerging evidence suggests developmental differences platelet immune functions. This study was designed compare the proteome phosphoproteome resting neonatal platelets. Methods We isolated umbilical cord blood-derived from healthy full term neonates (n=9) blood adults (n=7), compared...
ABSTRACT Colonization of human skin and nares by methicillin-resistant Staphylococcus aureus (MRSA) leads to the community spread MRSA. This is exacerbated transfer MRSA between humans livestock, particularly swine. Here, we capitalized on shared features porcine skin, including colonization, study novel bacterial mediators colonization resistance. We focused poorly studied species Desemzia incerta , which found exert antimicrobial activity through a secreted product exhibited resistance...
Recurrent infections are a hallmark of STAT3 dominant-negative hyper-IgE syndrome (STAT3 HIES), rare immunodeficiency previously known as Jobs syndrome, along with elevated IgE levels and impaired neutrophil function. We have been developing nanoparticles trophism that home to the sites infection via these first-responder leukocytes, named neutrophil-avid nanocarriers (NANs). Here, we demonstrate human neutrophils can phagocytose nanogels (NGs), type NAN, enhanced uptake after particle serum...
Neurogenic bladder poses a major morbidity in children with spina bifida (SB), and videourodynamic studies (VUDS) are used to stratify this risk. This small-scale pilot study utilized current mass-spectrometry-based proteomic approaches identify peptides or proteins urine that may differentiate at high risk of developing renal complications from neurogenic bladder. Twenty-two samples which nine had pressure storage put the upper urinary tract risk, while 13 lower for compromise were...
ABSTRACT Colonization of human skin and nares by methicillin-resistant Staphylococcus aureus (MRSA) leads to community spread MRSA. This is exacerbated transfer MRSA between humans livestock, particularly swine. Here we capitalized on the shared features porcine skin, including colonization, study novel bacterial mediators colonization resistance. We focused poorly studied species Desemzia incerta , which found exert antimicrobial activity through a secreted product exhibited resistance...