A5055404631- Lipid metabolism and biosynthesis
- Lipid metabolism and disorders
- Peroxisome Proliferator-Activated Receptors
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cholesterol and Lipid Metabolism
- Cancer, Lipids, and Metabolism
- Liver Disease Diagnosis and Treatment
- Lipoproteins and Cardiovascular Health
- Endoplasmic Reticulum Stress and Disease
- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- Diet, Metabolism, and Disease
- Circadian rhythm and melatonin
- RNA modifications and cancer
- Pancreatic function and diabetes
- Dietary Effects on Health
- Drug Transport and Resistance Mechanisms
- Cancer-related molecular mechanisms research
- Nutrition, Genetics, and Disease
- Metabolomics and Mass Spectrometry Studies
- Retinoids in leukemia and cellular processes
- Diabetes Treatment and Management
- Antioxidant Activity and Oxidative Stress
- Atherosclerosis and Cardiovascular Diseases
- RNA Research and Splicing
Long Island University
2019-2025
Winthrop-University Hospital
2016-2024
SUNY Downstate Health Sciences University
2015-2024
Centre for Genomic Regulation
2024
Barcelona Institute for Science and Technology
2024
New York University
2018-2024
VA NY Harbor Healthcare System
2014-2023
University of Faisalabad
2022
University of Management and Technology
2022
State University of New York
2010-2019
BACKGROUND A growing body of evidence from epidemiological data, animal studies, and clinical trials supports HDL as the next target to reduce residual cardiovascular risk in statin-treated, high-risk patients. For more than 3 decades, cholesterol has been employed principal measure associated with low HDL-cholesterol concentrations. The physicochemical functional heterogeneity present important challenges investigators field who are seeking identify effective laboratory methods develop a...
Microsomal triglyceride transfer protein (MTP) was first identified as a major cellular capable of transferring neutral lipids between membrane vesicles. Its role an essential chaperone for the biosynthesis apolipoprotein B (apoB)-containing triglyceride-rich lipoproteins established after realization that abetalipoproteinemia patients carry mutations in MTTP gene resulting loss its lipid activity. Now it is known also plays CD1, glycolipid presenting molecules, well regulation cholesterol...
Abstract Vertebrates transport hydrophobic triglycerides through the circulatory system by packaging them within amphipathic particles called Triglyceride-Rich Lipoproteins. Yet, it remains largely unknown how are loaded onto these particles. Mutations in Phospholipase A2 group 12B ( PLA2G12B) known to disrupt lipoprotein homeostasis, but its mechanistic role this process unclear. Here we report that PLA2G12B channels lipids lumen of endoplasmic reticulum into nascent lipoproteins. This...
Addition of apolipoprotein (apo) E to rabbit beta-very low density lipoproteins (beta-VLDL) has been shown result in a marked enhancement their binding and uptake by various cell types. Apolipoprotein binds lipoprotein receptors proteoglycans. To distinguish between apoE these sites, cells were treated with heparinase. Heparinase treatment receptor-negative familial hypercholesterolemic (FH) fibroblasts human hepatoma (HepG2) released 30-40% newly synthesized surface 35S-labeled...
In presence of oleate and taurocholate, differentiated CaCo-2 cell monolayers on membranes were able to assemble secrete chylomicrons. Under these conditions, both cellular uptake secretion into chylomicrons β-carotene (β-C) curvilinear, time-dependent (2–16 h), saturable, concentration-dependent (apparent Km 7–10 μM) processes. linear concentration conditions at 16 h incubation, the extent absorption all-trans β-C was 11% (80% in chylomicrons), while those 9-cis- 13-cis-β-C significantly...
Clock genes respond to external stimuli and exhibit circadian rhythms. This study investigated the expression of clock in small intestine their contribution regulation nutrient absorption by enterocytes. We examined macronutrient transport proteins intestines wild-type mutant (Clk(mt/mt)) mice with free or limited access food. In addition, we studied macronutrients these mice. Intestinal show food entrainment Dominant negative Clk(mt/mt) disrupts genes. The lipids monosaccharides was high...
The involvement of the low density lipoprotein receptor-related protein (LRP) in chylomicron remnant (CR) catabolism was investigated.Ligand blot analyses demonstrated that B-very lipoproteins (8-VLDL) incubated with apolipoprotein E (8-VLDL+E) bound to LRP and receptors, whereas active (receptor-binding) az- macroglobulin (azM) only partially purified from rat liver membranes.Iodinated 6-VLDL+E azM showed high affinity binding LRP/azM receptor receptornegative fibroblasts.The degradation...
Despite three decades of extensive studies on human apolipoprotein A-I (apoA-I), the major protein component in high-density lipoproteins, molecular basis for its antiatherogenic function is elusive, part because lack a structure full-length protein. We describe here crystal lipid-free apoA-I at 2.4 Å. The shows that comprised an N-terminal four-helix bundle and two C-terminal helices. domain plays prominent role maintaining conformation, indicating mutants with truncations this region form...
Transintestinal cholesterol excretion (TICE) is an alternate pathway to hepatobiliary secretion. Our study aimed at identifying molecular mechanisms of TICE.We studied TICE ex vivo in mouse and human intestinal explants, after bile diversion cannulation mice. We provide the first evidence that both low-density lipoprotein (LDL) high-density deliver for jejunal explants basolateral side. Proprotein convertase subtilisin kexin type 9 (PCSK9)(-/-) mice show increased LDL-TICE, acute injection...
Cell and organelle membranes consist of a complex mixture phospholipids (PLs) that determine their size, shape, function. Phosphatidylcholine (PC) is the most abundant phospholipid in eukaryotic membranes, yet how cells sense regulate its levels vivo remains unclear. Here we show PCYT1A, rate-limiting enzyme PC synthesis, intranuclear re-locates to nuclear membrane response need for PL synthesis yeast, fly, mammalian cells. By aligning imaging with lipidomic analysis data-driven modeling,...
Circadian rhythms controlled by clock genes affect plasma lipids. Here we show that global ablation of Bmal1 in Apoe-/- and Ldlr-/- mice its liver-specific (L-Bmal1-/-Apoe-/-) increases, whereas overexpression BMAL1 L-Bmal1-/-Apoe-/- Apoe-/-mice decreases hyperlipidaemia atherosclerosis. deficiency augments hepatic lipoprotein secretion diminishes cholesterol excretion to the bile. Further, reduces expression Shp Gata4. Reductions increase Mtp production, reductions Gata4 diminish...
Microsomal triglyceride transfer protein (MTP) is essential for the assembly of lipoproteins. MTP has been shown on surface lipid droplets adipocytes; however its function in adipose tissue not well defined. We hypothesized that may play critical role droplet formation and expansion.Plasmids mediated overexpression siRNA knockdown Mttp gene were performed 3T3-L1 pre-adipocytes to evaluate effects cell differentiation accumulation. Adipose-specific was achieved mice bybreeding floxed (Mttp...