A5085311231- Insect and Pesticide Research
- Lipid metabolism and disorders
- Lipid metabolism and biosynthesis
- Nanoparticles: synthesis and applications
- Neurobiology and Insect Physiology Research
- Essential Oils and Antimicrobial Activity
- Plant and animal studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Insect and Arachnid Ecology and Behavior
- Phytochemistry and Biological Activities
- Peroxisome Proliferator-Activated Receptors
- Cholesterol and Lipid Metabolism
- Endoplasmic Reticulum Stress and Disease
- Insect-Plant Interactions and Control
- Analytical Chemistry and Chromatography
- Marine Sponges and Natural Products
- Seaweed-derived Bioactive Compounds
- Analytical chemistry methods development
- Smart Agriculture and AI
- Insect Pest Control Strategies
- Spectroscopy and Chemometric Analyses
- Moringa oleifera research and applications
- Bee Products Chemical Analysis
- Soybean genetics and cultivation
- Bioactive Compounds and Antitumor Agents
Madurai Medical College
2024
REVA University
2022-2024
SASTRA University
2014-2024
SRM Institute of Science and Technology
2018-2021
AAA College of Engineering and Technology
2021
Bristol-Myers Squibb (India)
2019-2020
Biocon (India)
2019-2020
Syngene International (India)
2019-2020
University of Ottawa
2008-2018
SRM University
2013-2018
AbstractUsing computerized video time-lapse microscope, we studied early cellular events during carcinogen-induced transformation of C3H10T1/2 cells. Multinucleate / polyploid giant cells (MN/PGs) formed due to DNA damage is thought die via mitotic catastrophe. Before they die, some MN/PGs undergo a novel type cell division, termed neosis, characterized by karyokinesis nuclear budding followed asymmetric, intracellular cytokinesis, producing several small mononuclear cells, the Raju with...
Excess lipid induced metabolic disorders are one of the major existing challenges for society. Among many different causes disorders, overproduction and compromised catabolism triacylglycerol-rich very low density lipoproteins (VLDL) have become increasingly prevalent leading to hyperlipidemia worldwide. This review provides latest understanding in aspects VLDL assembly process, including structure-function relationships within apoB, mutations APOB causing hypobetalipoproteinemia,...
Hepatic assembly of triacylglycerol (TAG)-rich very low density lipoproteins (VLDL) is achieved through recruitment bulk TAG (presumably in the form lipid droplets within microsomal lumen) into VLDL precursor containing apolipoprotein (apo) B-100. We determined protein/lipid components lumenal (LLD) cells expressing recombinant human apoC-III (C3wt) or a mutant (K58E, C3KE) initially identified humans that displayed hypotriglyceridemia. Although expression C3wt markedly stimulated secretion...
Phosphatidate phosphatase-1 (PAP-1) converts phosphatidate to diacylglycerol and plays a key role in the biosynthesis of phospholipids triacylglycerol (TAG). PAP-1 activity is encoded by members lipin family, including lipin-1 (1α 1β), -2, -3. We determined effect expression on assembly secretion very low density lipoproteins (VLDL) using McA-RH7777 cells. Expression lipin-1α or -1β increased synthesis [3H]glycerol-labeled lipids under either basal- oleate-supplemented conditions. In...
Apolipoprotein (apo) C-III plays a regulatory role in VLDL lipolysis and clearance. In this study, we determined potential intracellular of apoC-III hepatic assembly secretion. Stable expression recombinant McA-RH7777 cells resulted increased secretion efficiency VLDL-associated triacylglycerol (TAG) apoB-100 gene-dosage-dependent manner. The stimulatory effect on TAG was manifested only when were cultured under lipid-rich (i.e., media supplemented with exogenous oleate) but not lipid-poor...
Five nontruncating missense APOB mutations, namely A31P, G275S, L324M, G912D, and G945S, were identified in heterozygous carriers of familial hypobetalipoproteinemia (FHBL) the Italian population. To test that FHBL phenotype was a result impaired hepatic secretion mutant apoB proteins, we performed transfection studies using McA-RH7777 cells stably expressing wild type or forms human apolipoprotein B-48 (apoB-48). All proteins displayed varied impairment secretion, with G912D least affected...
We have shown that expression of apolipoprotein (apo) C-III promotes VLDL secretion from transfected McA-RH7777 cells under lipid-rich conditions. To determine structural elements within apoC-III confer to this function, we contrasted wild-type with a mutant Ala23Thr originally identified in hypotriglyceridemia subjects. Although synthesis [(3)H]glycerol-labeled TAG was comparable between expressing (C3wt cells) or (C3AT cells), [(3)H]TAG C3AT markedly decreased. The lowered associated an...
In current scenario of human health and diseases, drug-induced hepatic injury has been recognized as a serious unresolved problem. Particularly, chemotherapeutic agents have reported to induce organ toxicity. The aim the present study is evaluate toxicity oxidative damage induced by cyclophosphamide (CP), drug its amelioration sesamol, an antioxidant from sesame seeds. CP (150 mg/kg) injected intraperitonially experimental rats day 2 were orally treated with sesamol. Rats sacrificed...
Recent cell culture and animal studies have suggested that expression of human apo C-III in the liver has a profound impact on triacylglycerol (TAG)-rich VLDL1 production under lipid-rich conditions. The apoC-III Gln38Lys variant was identified subjects Mexican origin with moderate hypertriglyceridemia. We postulated (C3QK), being gain-of-function mutation, promotes hepatic assembly/secretion. To test this hypothesis, we expressed C3QK McA-RH7777 cells apoc3-null mice to contrast its effect...