A5015191570- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Connexins and lens biology
- Neural dynamics and brain function
- Visual perception and processing mechanisms
- Neuroscience and Neural Engineering
- Ocular and Laser Science Research
- Zebrafish Biomedical Research Applications
- Retinal Diseases and Treatments
- Impact of Light on Environment and Health
- Glaucoma and retinal disorders
- Neurobiology and Insect Physiology Research
- Nicotinic Acetylcholine Receptors Study
- Circadian rhythm and melatonin
- Ion channel regulation and function
- Photochromic and Fluorescence Chemistry
- Animal testing and alternatives
- Color Science and Applications
- Physiological and biochemical adaptations
- Neuroinflammation and Neurodegeneration Mechanisms
- Fish biology, ecology, and behavior
- Vestibular and auditory disorders
- Ion Channels and Receptors
- Neural Networks and Applications
Netherlands Institute for Neuroscience
2014-2023
University of Amsterdam
2013-2023
Amsterdam UMC Location University of Amsterdam
2011-2023
Royal Netherlands Academy of Arts and Sciences
2004-2022
Amsterdam University Medical Centers
2021
Royal Academy of Art
2020-2021
University of Nottingham
2021
Amsterdam UMC Location Vrije Universiteit Amsterdam
2020
Signal Processing (United States)
2010-2011
Amsterdam Neuroscience
2009
An animal's ability to survive depends on its sensory systems being able adapt a wide range of environmental conditions, by maximizing the information extracted and reducing noise transmitted. The visual system does this adapting luminance contrast. While adaptation can begin at retinal photoreceptors, contrast has been shown start later stages in retina. Photoreceptors changes over multiple time scales ranging from tens milliseconds minutes, with adaptive arising processes within...
An essential feature of the first synapse in retina is a negative feedback pathway from horizontal cells to cones. Here we show that at this synapse, connexin26 forms hemichannels on cell dendrites near glutamate release site Blocking these hyperpolarizes cells, modulates Ca 2+ channels cones, and abolishes all feedback-mediated responses. We propose mechanism which activity subsequent cones are modulated by current through hemichannels. Because depends polarization their output
On bipolar cells are connected to photoreceptors via a sign-inverting synapse. At this synapse, glutamate binds metabotropic receptor which couples the closure of cation-selective transduction channel. The molecular identity both and G protein known, but channel has remained elusive. Here, we show that in mouse rod cells, subtype cell, is likely be member TRP family channels. To evoke current, antagonist LY341495 was applied dendrites were bathed solution containing mGluR6 agonists l -AP4 or...
Vanishing white matter (VWM) is a fatal leukodystrophy that caused by mutations in genes encoding subunits of eukaryotic translation initiation factor 2B (eIF2B). Disease onset and severity are codetermined genotype. White astrocytes oligodendrocytes almost exclusively affected; however, the mechanisms VWM development remain unclear. Here, we used mouse models, patients' tissue, cell cultures to investigate whether or primary affected type. We generated 2 models with...
Mutations in the NYX gene that encodes protein nyctalopin cause congenital stationary night blindness type 1. In no b-wave (nob) mice, a mutation Nyx results functional phenotype includes absence of electroretinogram and abnormal spontaneous light-evoked activity retinal ganglion cells (RGCs). contrast, there is morphological abnormality retina at either light or electron microscopic levels. These deficits suggest required for normal synaptic transmission between photoreceptors depolarizing...
In the vertebrate retina, horizontal cells generate inhibitory surround of bipolar cells, an essential step in contrast enhancement. For last decades, mechanism involved this synaptic pathway has been a major controversy retinal research. One hypothesis suggests that connexin hemichannels mediate negative feedback signal; another is mediated by protons. Mutant zebrafish were generated lack 55.5 cells. Whole cell voltage clamp recordings made from isolated and cones flat mount retinas....
A slow mechanism of retinal synaptic inhibition involves hydrolysis ATP released from pannexin 1 channels (from the tips horizontal cell dendrites); resulting protons and phosphates acidify cleft, which inhibits neurotransmitter release.
Neurophysiological studies depend on a reliable quantification of whether and when neuron responds to stimulation. Simple methods determine responsiveness require arbitrary parameter choices, such as binning size, while more advanced model-based fitting hyperparameter tuning. These choices can change the results, which invites bad statistical practice reduces replicability. New recording techniques that yield increasingly large numbers cells would benefit from test for cell-inclusion...
Horizontal cells (HCs) appear to release, and also be sensitive to, GABA. The external GABA concentration is increased with depolarization of the HC membrane via an electrogenic transporter. This extracellular opens a GABAA-gated Cl- channel in membrane. Since equilibrium potential for (ECl) near -20 mV, released by further depolarizes HC. transporter GABAA receptor thus constitute positive feedback loop can slow down kinetics light responses HCs. affect receptor, probably because diffusion...
Background Recent studies designed to identify the mechanism by which retinal horizontal cells communicate with cones have implicated two processes. According one account, cell hyperpolarization induces an increase in pH within synaptic cleft that activates calcium current (Ca2+-current) cones, enhancing transmitter release. An alternative account suggests increases Ca2+-current promote release through a hemichannel-mediated ephaptic mechanism. Methodology/Principal Findings To distinguish...
Objective Megalencephalic leukoencephalopathy with cysts (MLC) is a genetic disease characterized by infantile onset white matter edema and delayed neurological deterioration. Loss of MLC1 function causes MLC. involved in ion–water homeostasis, but its exact role unknown. We generated Mlc1 ‐null mice for further studies. Methods investigated which brain cell types express MLC1, compared developmental expression men, studied the consequences loss mice. Results Like humans, expressed only...
Pontocerebellar hypoplasia (PCH) represents a group (PCH1–6) of neurodegenerative autosomal recessive disorders characterized by and/or atrophy the cerebellum, ventral pons, progressive microcephaly and variable neocortical atrophy. The majority PCH2 PCH4 cases are caused mutations in TSEN54 gene; one four subunits comprising tRNA-splicing endonuclease (TSEN) complex. We hypothesized that act through loss function mechanism. At 8 weeks gestation, human is expressed ubiquitously brain, yet...