A5100622245- Single-cell and spatial transcriptomics
- Retinal Development and Disorders
- Circadian rhythm and melatonin
- Sleep and Wakefulness Research
- Congenital heart defects research
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Insect and Pesticide Research
- Regulation of Appetite and Obesity
- Neonatal Respiratory Health Research
- Zebrafish Biomedical Research Applications
- Photoreceptor and optogenetics research
- Chromosomal and Genetic Variations
- Insect and Arachnid Ecology and Behavior
- Birth, Development, and Health
- Bee Products Chemical Analysis
- Ophthalmology and Eye Disorders
- Ecology and Conservation Studies
- Immune cells in cancer
- Plant and animal studies
- Genetic Mapping and Diversity in Plants and Animals
- Neural dynamics and brain function
- Fungal and yeast genetics research
Aarhus University
2023-2025
Johns Hopkins Medicine
2017-2024
Johns Hopkins University
2017-2024
Rural Development Administration
2023-2024
Danish Diabetes Academy
2024
University of Georgia
2016-2023
Soonchunhyang University Hospital
2012-2023
H. L. Snyder Medical Foundation
2021
Discovery Institute
2020
National Institute of Agricultural Science and Technology
2019
We report de novo genome assemblies, transcriptomes, annotations, and methylomes for the 26 inbreds that serve as founders maize nested association mapping population. The number of pan-genes in these diverse genomes exceeds 103,000, with approximately a third found across all genotypes. results demonstrate ancient tetraploid character continues to degrade by fractionation present day. Excellent contiguity over repeat arrays complete annotation centromeres revealed additional variation major...
Injury induces retinal Müller glia of certain cold-blooded vertebrates, but not those mammals, to regenerate neurons. To identify gene regulatory networks that reprogram into progenitor cells, we profiled changes in expression and chromatin accessibility from zebrafish, chick, mice response different stimuli. We identified evolutionarily conserved species-specific controlling glial quiescence, reactivity, neurogenesis. In zebrafish the transition quiescence reactivity is essential for...
Plant cell death plays important roles during plant-pathogen interactions. To study pathogen-induced death, there is a need for cytological tools that allow determining not only host viability, but also cellular events leading to with visualization of pathogen development. Here we describe live imaging method provide insights into the dynamics in rice (Oryza sativa). This uses live-cell confocal microscopy sheath cells mechanically damaged or invaded by fluorescently-tagged Magnaporthe...
The hypothalamus is a central regulator of many innate behaviors essential for survival, but the molecular mechanisms controlling hypothalamic patterning and cell fate specification are poorly understood. To identify genes that control development, we have used single-cell RNA sequencing (scRNA-Seq) to profile mouse gene expression across 12 developmental time points between embryonic day 10 postnatal 45. This identified delineated clear trajectories all major types, readily distinguished...
It is well-accepted that maternal obesity affects fetal development to elevate the risk of offspring disease, but how this happens unclear. Understanding placental alterations during gestation as a consequence critical understanding impact on programming. Here, we used histological criteria, flow cytometry, quantitative PCR, and multiplex cytokine assays examine changes in cell proliferation inflammation placenta mouse model high-fat diet-induced obesity. We focused mid- late (approximately...
Gene regulatory networks (GRNs), consisting of transcription factors and their target sites, control neurogenesis cell-fate specification in the developing central nervous system. In this study, we use integrated single-cell RNA ATAC sequencing (scATAC-seq) analysis mouse human retina to identify multiple interconnected, evolutionarily conserved GRNs composed cell-type-specific that both activate genes within own network inhibit other networks. These temporal patterning primary progenitors,...
Retinal ganglion cells (RGCs) relay visual information from the eye to brain. RGCs are first cell type generated during retinal neurogenesis. Loss of function transcription factor Atoh7, expressed in multipotent early neurogenic progenitors leads a selective and essentially complete loss RGCs. Therefore, Atoh7 is considered essential for conferring competence on generate Despite importance RGC specification, we find that inhibiting apoptosis Atoh7-deficient mice by Bax only modestly reduces...
Direct reprogramming of glia into neurons is a potentially promising approach for the replacement lost to injury or neurodegenerative disorders. Knockdown polypyrimidine tract-binding protein Ptbp1 has been recently reported induce efficient conversion retinal Mϋller functional neurons. Here, we use combination genetic lineage tracing, single-cell RNA sequencing (scRNA-seq), and electroretinogram analysis show that selective induction either heterozygous homozygous loss-of-function mutants...
Amongst risk alleles associated with late-onset Alzheimer's disease (AD), those that converged on the regulation of microglia activity have emerged as central to progression. Yet, how canonical amyloid-β (Aβ) and tau pathologies regulate subtypes during progression AD remains poorly understood.We use single-cell RNA-sequencing profile from mice exhibiting both Aβ across We identify novel are induced in response a disease-stage-specific manner. To validate observation mouse models, we also...
Pure neuronal and mixed neuronal-glial tumors of the central nervous system are uncommon but fascinating because they less aggressive than more common glial their prognosis is excellent. Neurologic manifestations varied include seizures, symptoms increased intracranial pressure, neurologic deficits according to tumor location. Many demonstrate characteristic radiologic findings. At magnetic resonance (MR) imaging, gangliocytomas low signal intensity on T1-weighted images, high T2-weighted...
Leptin is secreted by adipocytes to regulate appetite and body weight. Recent studies have reported that tanycytes actively transport circulating leptin across the brain barrier into hypothalamus, are required for normal levels of hypothalamic signaling. However, direct evidence receptor (LepR) expression lacking, effect tanycyte-specific deletion LepR has not been investigated. In this study, we analyze function tanycytic in mice. Using single-molecule fluorescent situ hybridization...
The ubiquitous presence of inhibitory interneurons in the thalamus primates contrasts with sparsity reported mice. Here, we identify a larger than expected complexity and distribution across mouse thalamus, where all thalamic can be traced back to two developmental programmes: one specified midbrain other forebrain. Interneurons migrate functionally distinct thalamocortical nuclei depending on their origin: abundant, midbrain-derived class populates first higher order sensory while rarer,...
The hypothalamus regulates many innate behaviors, but its development remains poorly understood. Here, we used single-cell RNA sequencing (RNA-seq) and hybridization chain reaction (HCR) to profile multiple stages of early hypothalamic in the chick. Hypothalamic neuroepithelial cells are initially induced from prethalamic-like cells. Two distinct progenitor populations then emerge give rise tuberal mammillary/paraventricular At later stages, regional organization chick mouse is highly...
The arcuate nucleus (ARC) regulates body weight in response to blood-borne signals of energy balance. Blood-brain barrier (BBB) permeability the ARC is determined by capillary endothelial cells (ECs) and tanycytes. Tight junctions between ECs limit paracellular entry molecules into brain, whereas EC transporters fenestrations regulate transcellular entry. Tanycytes appear form a that prevents free diffusion molecules. Here we tested hypothesis gestation an obese mother alters BBB offspring....
Abstract GABAergic neurons of the hypothalamus regulate many innate behaviors, but little is known about mechanisms that control their development. We previously identified hypothalamic express LIM homeodomain transcription factor Lhx6, a master regulator cortical interneuron development, as sleep-promoting. In contrast to telencephalic interneurons, Lhx6 do not undergo long-distance tangential migration and interneuronal markers such Pvalb . Here, we show necessary for survival neurons....
Maternal obesity during pregnancy increases the risk of in offspring. Several observations have pointed to a causative role for proinflammatory cytokine IL-6, but whether it is present fetal circulation and how acts on developing fetus are unclear. We first observed that postnatal day 0 offspring from obese mothers had significantly reduced neuropeptide Y (NPY) innervation paraventricular nucleus (PVN) compared with normal-weight controls. Thus, growth NPY neurites arcuate (ARC) was impaired...
Abstract Maternal obesity during pregnancy is associated with chronic maternal, placental, and fetal inflammation; it elevates the risk for offspring obesity. Changes in development of hypothalamus, a brain region that regulates body weight energy balance, are emerging as important determinants risk, but such changes only beginning to be defined. Here we focused on hypothesis pathological exposure developing hypothalamic astrocytes cytokines would alter their development. A maternal high‐fat...
ABSTRACT Synovial joint development begins with the formation of interzone, a region condensed mesenchymal cells at site prospective joint. Recently, lineage-tracing strategies have revealed that Gdf5-lineage native to and from outside interzone contribute most, if not all, major components. However, there is limited knowledge specific transcriptional signaling programs regulate fate diversification synovial constituents. To address this, we performed single cell RNA-Seq analysis 7329...
The tuberal hypothalamus controls life-supporting homeostatic processes, but despite its fundamental role, the cells and signalling pathways that specify this unique region of central nervous system in embryogenesis are poorly characterised. Here, we combine experimental bioinformatic approaches embryonic chick to show is progressively generated from hypothalamic floor plate-like cells. Fate-mapping studies a stream progenitors develops anterior-ventral neural tube as wave...
The forebrain is the most complex region of vertebrate CNS, and its developmental organisation controversial. We fate-mapped embryonic chick using lipophilic dyes Cre-recombination lineage tracing, built a 4D model brain growth. reveal modular patterns anisotropic growth, ascribed to progenitor regions through multiplex HCR. Morphogenesis dominated by directional growth towards eye, more isometric expansion prethalamus dorsal telencephalon, anterior movement ventral cells into hypothalamus....
Summary Neuronal subtypes derived from the embryonic hypothalamus and prethalamus regulate many essential physiological processes, yet gene regulatory networks controlling their development remain poorly understood. Using single-cell RNA- ATAC-sequencing, we analyzed mouse hypothalamic prethalamic day 11 to postnatal 8, profiling 660,000 cells in total. This identified key transcriptional chromatin dynamics driving regionalization, neurogenesis, differentiation. multiple distinct neural...