A5045066293- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Hearing, Cochlea, Tinnitus, Genetics
- Digital Holography and Microscopy
- Congenital heart defects research
- Advanced Fluorescence Microscopy Techniques
- Neonatal and fetal brain pathology
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Olfactory and Sensory Function Studies
- Plant and Biological Electrophysiology Studies
- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Developmental Biology and Gene Regulation
- Receptor Mechanisms and Signaling
- Neonatal Respiratory Health Research
- Photoreceptor and optogenetics research
Johns Hopkins University
2021-2025
Johns Hopkins Medicine
2025
Soka University
2018
Summary Neuronal subtypes derived from the embryonic hypothalamus and prethalamus regulate many essential physiological processes, yet gene regulatory networks controlling their development remain poorly understood. Using single-cell RNA- ATAC-sequencing, we analyzed mouse hypothalamic prethalamic day 11 to postnatal 8, profiling 660,000 cells in total. This identified key transcriptional chromatin dynamics driving regionalization, neurogenesis, differentiation. multiple distinct neural...
Subplate neurons (SpNs) are among the earliest generated cortical that form functional synapses, and aid in circuit development. A fraction of SpNs survive layer 6b adult cortex. While exhibit a large variety molecular identities, it is unclear if identity correlates with or connectomic identities different have similar developmental trajectories. To resolve these questions, we here characterize intracortical circuits to molecularly identified subpopulations SpN-specific Cre-lines...
Abstract Subplate neurons (SpNs) are a heterogeneous neuronal population actively involved in early cortical circuit formation. In rodents, many SpNs survive and form layer 6b. The molecular heterogeneity of raises the question whether different subpopulations through postnatal period similarly such diverse SpN populations auditory cortex (ACtx) share common distribution pattern with other sensory systems. To address that, we investigated expression multiple specific markers ACtx, as well...
Fundamental understanding of large-scale dynamic connectivity within a living organism requires volumetric imaging over large field view (FOV) at biologically relevant speed and resolution. However, most microscopy methods make trade-offs between FOV axial resolution, making it challenging to observe highly processes cellular resolution in 3D across mesoscopic scales (e.g., whole zebrafish larva). To overcome this limitation, we have developed oblique plane (Meso-OPM) with diffractive light...
Abstract Proper formation of laminar structures in sensory cortexes is critical for information processing. Previous studies suggested that the timing neuronal migration and position cortical neurons differ among cortexes. How they during postnatal development has not been systematically investigated. Here, identifying laminas using transcription factors, we examined changes density distribution presumptive primary auditory (ACx), visual (VCx), somatosensory (SCx) a strain mice...
Abstract Sensory perturbation in one modality results the adaptive reorganization of neural pathways within spared modalities, a phenomenon known as “crossmodal plasticity,” which has been examined during or after classic “critical period.” Because peripheral perturbations can alter auditory cortex (ACX) activity and functional connectivity ACX subplate neurons (SPNs) even before critical period, called precritical we investigated if retinal deprivation at birth crossmodally alters SPN...
Abstract Neurons in the cerebral cortex form excitatory and inhibitory circuits with specific laminar locations. The mechanisms underlying development of these spatially is not fully understood. To test if postsynaptic N -methyl-D-aspartate (NMDA) receptors on neurons are required for to neurons, we genetically ablated NMDA from a subset temporal association (TeA) through utero electroporation assessed intracortical connecting L5 vitro whole-cell patch clamp recordings coupled laser-scanning...
Abstract Fundamental understanding of large-scale dynamic connectivity within a living organism requires volumetric imaging over large field view (FOV) at biologically relevant speed and resolution. However, most microscopy methods make trade-offs between FOV depth resolution, making it challenging to observe highly processes cellular resolution in 3D across mesoscopic scales (e.g., whole zebrafish larva). To overcome this limitation, we have developed oblique plane (Meso-OPM) with...
Sensory perturbation in one modality results adaptive reorganization of neural pathways within the spared modalities, a phenomenon known as "crossmodal plasticity", which has been examined during or after classic 'critical period'. Because peripheral perturbations can alter auditory cortex (ACX) activity and functional connectivity ACX subplate neurons (SPNs) even before critical period, called precritical we investigated if retinal deprivation at birth crossmodally alters SPN circuits...