A5064735343- Genetics, Aging, and Longevity in Model Organisms
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Single-cell and spatial transcriptomics
- Neurogenesis and neuroplasticity mechanisms
- 3D Printing in Biomedical Research
- Image Processing Techniques and Applications
- Retinal Development and Disorders
- Physiological and biochemical adaptations
- Photoacoustic and Ultrasonic Imaging
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
- Congenital heart defects research
- RNA Research and Splicing
- Neuroscience and Neural Engineering
- Spaceflight effects on biology
- Neuroinflammation and Neurodegeneration Mechanisms
- Cellular Mechanics and Interactions
- Photosynthetic Processes and Mechanisms
- Circadian rhythm and melatonin
- Regulation of Appetite and Obesity
- Cell Adhesion Molecules Research
- Neonatal Respiratory Health Research
Johns Hopkins Medicine
2021-2025
Johns Hopkins University
2021-2025
Yale University
2019-2021
Loss of function NFI transcription factors in hypothalamic tanycytes generates diverse subtypes functional neurons.
Summary Neuronal subtypes derived from the embryonic hypothalamus and prethalamus regulate many essential physiological processes, yet gene regulatory networks controlling their development remain poorly understood. Using single-cell RNA- ATAC-sequencing, we analyzed mouse hypothalamic prethalamic day 11 to postnatal 8, profiling 660,000 cells in total. This identified key transcriptional chromatin dynamics driving regionalization, neurogenesis, differentiation. multiple distinct neural...
Proliferative vitreoretinopathy (PVR) is the most common cause of failure retinal reattachment surgery, and molecular changes leading to this aberrant wound healing process are currently unknown. Our ultimate goal study PVR pathogenesis by employing single-cell transcriptomics dissect cellular heterogeneity.
Abstract Precise and reliable cell-specific gene delivery remains technically challenging. Here we report a splicing-based approach for controlling expression whereby separate translational reading frames are coupled to the inclusion or exclusion of mutated, frameshifting alternative exons. Candidate exons identified by analyzing thousands publicly available RNA sequencing datasets filtering cell specificity, conservation, local intron length. This method, which denote splicing-linked design...
Caenorhabditis elegans (C. elegans) stands out as the only organism in which challenge of understanding cellular origins an entire nervous system can be observed, with single cell resolution, vivo. Here, we present integrated protocol for examination neurodevelopment C. embryos. Our combines imaging, lineaging and neuroanatomical tracing cells developing We achieve long-term, four-dimensional (4D) imaging living embryos nearly isotropic spatial resolution through use Dual-view Inverted...
During development, neurites and synapses segregate into specific neighborhoods or layers within nerve bundles. The developmental programs guiding placement of in layers, hence their incorporation circuits, are not well understood. We implement novel imaging methods quantitative models to document the embryonic development C. elegans brain neuropil, discover that differential adhesion mechanisms control precise single onto layers. Differential is orchestrated via developmentally regulated...
Abstract We describe theoretical and practical advances in algorithm software design, resulting ten to several thousand-fold faster deconvolution multiview fusion than previous methods. First, we adapt methods from medical imaging, showing that an unmatched back projector accelerates Richardson-Lucy by at least 10-fold, most cases requiring only a single iteration. Second, show improvements 3D image-based registration with GPU processing result speedups of 10-100-fold over CPU processing....
Structured Abstract Purpose Proliferative vitreoretinopathy (PVR) is the most common cause of failure retinal reattachment surgery and molecular changes leading to this aberrant wound healing process currently unknown. We aimed study PVR pathogenesis using single-cell transcriptomics dissect cellular heterogeneity in a rabbit model. Methods was induced unilaterally Dutch Belted rabbits. At different timepoints following induction, retinas were dissociated into either cells or nuclei...
Summary We enhance the performance of confocal microscopy over imaging scales spanning tens nanometers to millimeters in space and milliseconds hours time, improving volumetric resolution more than 10-fold while simultaneously reducing phototoxicity. achieve these gains via an integrated, four-pronged approach: 1) developing compact line-scanners that enable sensitive, rapid, diffraction-limited large areas; 2) combining line-scanning with multiview imaging, reconstruction algorithms improve...
Caenorhabditis elegans (C. elegans) stands out as the only organism in which challenge of understanding cellular origins an entire nervous system can be observed, with single cell resolution, vivo. Here, we present integrated protocol for examination neurodevelopment C. embryos. Our combines imaging, lineaging and neuroanatomical tracing cells developing We achieve long-term, four-dimensional (4D) imaging living embryos nearly isotropic spatial resolution through use Dual-view Inverted...
Summary Neuropil is a fundamental form of tissue organization within brains 1 . In neuropils, densely packed neurons synaptically interconnect into precise circuit architecture 2,3 , yet the structural and developmental principles governing nanoscale precision in bundled neuropil assembly remain largely unknown 4–6 Here we use diffusion condensation, coarse-graining clustering algorithm 7 to identify nested structures C. elegans cerebral (called nerve ring). We determine that ring organized...
Abstract During development, neurites and synapses segregate into specific neighborhoods or layers within nerve bundles. The developmental programs guiding placement of in layers, hence their incorporation circuits, are not well understood. We implement novel imaging methods quantitative models to document the embryonic development C. elegans brain neuropil, discover that differential adhesion mechanisms control precise single onto layers. Differential is orchestrated via...