A5030002222- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Horticultural and Viticultural Research
- Cardiomyopathy and Myosin Studies
- Erythrocyte Function and Pathophysiology
- Engineering and Materials Science Studies
- Plant Reproductive Biology
- Cell Image Analysis Techniques
- Cell Adhesion Molecules Research
- Connexins and lens biology
- Microtubule and mitosis dynamics
- 3D Printing in Biomedical Research
- Metabolomics and Mass Spectrometry Studies
- Vehicle Dynamics and Control Systems
- Medical and Health Sciences Research
- Plant and animal studies
- Photoacoustic and Ultrasonic Imaging
- Medicinal plant effects and applications
- Muscle Physiology and Disorders
- Optical Coherence Tomography Applications
- Biotin and Related Studies
- Blood properties and coagulation
- Scientific Computing and Data Management
- Skin and Cellular Biology Research
- Historical Medical Research and Treatments
National Heart Lung and Blood Institute
2016-2025
National Institutes of Health
2015-2024
St. Vincent Charity Hospital
2022
Roger Williams Medical Center
2019-2022
Providence College
2019-2022
Boston University
2022
American College
2019
RWTH Aachen University
2016-2017
Anstalt für Verbrennungskraftmaschinen List (Austria)
2012-2016
Federal Institute of Education, Science and Technology Alagoas
2016
Cell-cell contact inhibition and the mechanical environment of cells have both been shown to regulate YAP nuclear localization modulate cell proliferation. Changes in cellular contractility by genetic, pharmacological, matrix stiffness perturbations localization. However, because F-actin organization are interconnected cytoskeletal properties, it remains unclear which these distinctly regulates Here we show that absence cell-cell contact, actomyosin suppresses phosphorylation at Ser(112),...
How adherent and contractile systems coordinate to promote cell shape changes is unclear. Here, we define a counterbalanced adhesion/contraction model for control. Live-cell microscopy data showed crucial role meshwork at the top of cell, which composed actin arcs myosin IIA filaments. The organized like muscle sarcomeres, with repeating II filaments separated by bundling protein α-actinin, mechanically coupled noncontractile dorsal fibers that run from bottom in cell. When contracts, it...
The actin cytoskeleton is locally regulated for functional specializations cell motility. Using quantitative fluorescent speckle microscopy (qFSM) of migrating epithelial cells, we previously defined two distinct F-actin networks based on their F-actin–binding proteins and patterns turnover movement. lamellipodium consists a treadmilling array with rapid polymerization-dependent retrograde flow contains high concentrations Arp2/3 ADF/cofilin, whereas the lamella exhibits spatially random...
Cell migration is initiated in response to biochemical or physical cues the environment that promote actin-mediated lamellipodial protrusion followed by formation of nascent integrin adhesions (NAs) within drive leading edge advance. Although FAK known be required for cell through effects on focal adhesions, its role NA and dynamics unclear. Live-cell microscopy −/− cells with expression phosphorylation deficient a FERM-domain mutant Arp2/3 binding revealed requirement promoting dense...
Significance Tumor progression to enable metastasis includes remodeling the wavy bundles of collagen making up tissue stromal extracellular matrix (ECM) into straight within tumor microenvironment. While are thought be inhibitory cell polarization and migration in tissue, ECM fibers conducive, thereby mediating metastasis. We used nanofabricated culture substrates that mimic fiber waveforms seen both benign- metastases-promoting ECMs. Large amplitude waves depolarized cells decreased...
Polarized fluorescence microscopy is a valuable tool for measuring molecular orientations in biological samples, but techniques recovering three-dimensional and positions of fluorescent ensembles are limited. We report polarized dual-view light-sheet system determining the diffraction-limited distribution dipoles that label structures. share set visualization, histogram, profiling tools interpreting these orientations. model distributions based on polarization-dependent efficiency excitation...
During angiogenesis, cytoskeletal dynamics that mediate endothelial cell branching morphogenesis during vascular guidance are thought to be regulated by physical attributes of the extracellular matrix (ECM) in a process termed mechanosensing. Here, we tested involvement microtubules linking mechanosensing morphogenesis. We used recently developed microtubule plus end–tracking program show specific parameters assembly dynamics, growth speed and persistence, globally regionally modified by,...
Although the role of actin cytoskeleton in morphogenesis polarized epithelial sheets is generally accepted as centrally important, regulation dynamics this process remains unclear. Here, we show that pointed-end capping protein Tmod3 contributes to cell shape within confluent monolayers cells. localizes lateral membranes epithelia several types. Reduction levels by shRNA leads a loss F-actin and tropomyosins from membranes, decrease height, without effects on localisation tight junction or...
The role of nonmuscle myosin 2 (NM2) pulsatile dynamics in generating contractile forces required for developmental morphogenesis has been characterized, but whether these contractions are an intrinsic property all actomyosin networks is not known. Here we used live-cell fluorescence imaging to show that transient, local assembly NM2A “pulses” occurs the cortical cytoskeleton single adherent cells mesenchymal, epithelial, and sarcoma origin, independent signaling cues cell–cell or cell–ECM...
Cancer cell migration during metastasis is mediated by a highly polarized cytoskeleton. MARK2 and its invertebrate homolog Par1B are kinases that regulate the microtubule cytoskeleton to mediate polarization of neurons in mammals embryos invertebrates. However, role cancer unclear. Using osteosarcoma cells, we found addition known localizations on microtubules plasma membrane, also associates with actomyosin focal adhesions. Cells depleted MARK proteins demonstrated promotes phosphorylation...
Tropomodulin1 (Tmod1) caps thin filament pointed ends in striated muscle, where it controls lengths by regulating actin dynamics. Here, we investigated myofibril assembly and heart development a Tmod1 knockout mouse. In the absence of Tmod1, embryonic appeared normal up to day (E) 8.5. By E9.5, defects were evident, including aborted myocardium inability pump, leading lethality E10.5. Confocal microscopy hearts E8–8.5 null embryos revealed structures resembling nascent myofibrils with...