A5033354962- Neural dynamics and brain function
- Visual perception and processing mechanisms
- Neurobiology and Insect Physiology Research
- Retinal Development and Disorders
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Face Recognition and Perception
- Optical Imaging and Spectroscopy Techniques
- Neuroscience and Neural Engineering
- Visual Attention and Saliency Detection
- Photoacoustic and Ultrasonic Imaging
- Multisensory perception and integration
- Neuroscience and Neuropharmacology Research
- Vestibular and auditory disorders
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Color perception and design
- Spatial Cognition and Navigation
- CCD and CMOS Imaging Sensors
- Categorization, perception, and language
- Neural Networks and Applications
- Neurogenesis and neuroplasticity mechanisms
- Amphibian and Reptile Biology
- Animal Vocal Communication and Behavior
- RNA and protein synthesis mechanisms
Allen Institute for Brain Science
2018-2024
Johns Hopkins University
2013-2024
Johns Hopkins Medicine
2018-2024
Discovery Institute
2022-2024
Kennedy Krieger Institute
2024
Salk Institute for Biological Studies
2007-2016
Max Planck Institute for Biological Cybernetics
2001-2008
Max Planck Society
2001-2008
Abstract Multiphoton microscopy can resolve fluorescent structures and dynamics deep in scattering tissue has transformed neural imaging, but applying this technique vivo be limited by the mechanical optical constraints of conventional objectives. Short working distance objectives collide with compact surgical windows or other instrumentation preclude imaging. Here we present an ultra-long (20 mm) air objective called Cousa objective. It is optimized for performance across multiphoton...
Neurons in the inferior temporal (IT) cortex respond selectively to complex objects, and maintain their selectivity despite partial occlusion. However, relatively little is known about how occlusion of different shape parts influences responses IT cortex. Here, we determine experimentally which objects monkeys are relying on a discrimination task. We then study effect with behavioral relevance neural at level spiking activity local field potentials (LFPs). For both LFPs, found that...
Humans and rhesus monkeys can identify shapes that have been rotated in the picture plane. Recognition of be as efficient recognition upright shapes. Here we investigate whether subjects showing view-invariant performance use same object features to versions a shape. We find marked differences between humans monkeys. While tend independent shape orientation, unique for each orientation. are able generalize greater degree across orientation changes than monkey observers, who relearn separate...
Area V4 is the first object-specific processing stage in ventral visual pathway, just as area MT motion-specific dorsal pathway. For almost 50 years, coding of object shape has been studied and conceived terms flat pattern processing, given its early position transformation 2D images. Here, however, awake monkey recording experiments, we found that roughly half neurons are more tuned responsive to solid, 3D shape-in-depth, conveyed by shading, specularity, reflection, refraction, or...
Abstract Precise and reliable cell-specific gene delivery remains technically challenging. Here we report a splicing-based approach for controlling expression whereby separate translational reading frames are coupled to the inclusion or exclusion of mutated, frameshifting alternative exons. Candidate exons identified by analyzing thousands publicly available RNA sequencing datasets filtering cell specificity, conservation, local intron length. This method, which denote splicing-linked design...
The neocortex varies in size and complexity among mammals due to the tremendous variability number diversity of neuronal subtypes across species. increased cellular is paralleled by expansion pool neocortical progenitors emergence indirect neurogenesis during brain evolution. molecular pathways that control these biological processes are disrupted neurological disorders remain largely unknown. Here we show transcription factors BRN1 BRN2 have an evolutionary conserved function their...
We studied the relative accuracy of drifting gratings and noise stimuli for functionally characterizing neural populations using two-photon calcium imaging. Calcium imaging has potential to distort measurements due nonlinearity in conversion from spikes observed fluorescence. demonstrate a dramatic impact fluorescence saturation on functional ferret V1 by showing that responses strongly violate contrast invariance orientation tuning, fundamental property spike rates. The relationship is...
Abstract Ferrets have become a standard animal model for the development of early visual stages. Less is known about higher-level vision in ferrets, both during and adulthood. Here, as step towards establishing research we used behavioral experiments to test motion form integration capacity adult ferrets. Motion was assessed by training ferrets discriminate random dot kinematograms (RDK) based on their direction. Task difficulty varied systematically changing RDK coherence levels, which...
When your head tilts laterally, as in sports, reaching, and resting, eyes counterrotate less than 20%, thus eye images rotate, over a total range of about 180°. Yet, the world appears stable vision remains normal. We discovered neural strategy for rotational stability anterior inferotemporal cortex (IT), final stage object primates. measured orientation tuning IT neurons macaque monkeys tilted +25 –25° producing ~40° difference retinal image orientation. Among with consistent tuning, 63%...
Viral vectors are promising tools for the dissection of neural circuits. In principle, they can manipulate neurons at a level specificity not otherwise achievable. While many studies have used viral vector-based approaches in rodent brain, only few employed this technique non-human primate, despite importance animal model neuroscience research. Here, we report evidence that approach be to monkey's behavior task. For purpose, allatostatin receptor/allatostatin (AlstR/AL) system, which has...
Abstract Multiphoton microscopy can resolve fluorescent structures and dynamics deep in scattering tissue, but applying this technique vivo be limited by short working distance water-immersion objectives. Here we present an ultra long (20 mm) air objective called the Cousa objective. It is optimized for performance across multiphoton imaging wavelengths, offers a > 4 mm 2 field-of-view with submicron lateral resolution, compatible commonly used systems. We share full optical prescription,...