A5102888725- RNA modifications and cancer
- RNA Research and Splicing
- Circular RNAs in diseases
- Virus-based gene therapy research
- Amyotrophic Lateral Sclerosis Research
- RNA Interference and Gene Delivery
- RNA regulation and disease
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Endoplasmic Reticulum Stress and Disease
- Plant nutrient uptake and metabolism
- Hedgehog Signaling Pathway Studies
- Antibiotic Resistance in Bacteria
- Cellular transport and secretion
- Protein Tyrosine Phosphatases
- Mycobacterium research and diagnosis
- RNA and protein synthesis mechanisms
- Pediatric Hepatobiliary Diseases and Treatments
- CRISPR and Genetic Engineering
- Diet and metabolism studies
- Gut microbiota and health
- Bacteriophages and microbial interactions
Johns Hopkins Medicine
2022-2025
Johns Hopkins University
2022-2025
Shanghai Sixth People's Hospital
2022
Shanghai Jiao Tong University
2022
Beijing University of Chinese Medicine
2022
Hainan Medical University
2020-2021
Southwest Medical University
2019
Syracuse University
2015
Tsinghua University
2014
N6-methyladenosine (m6A) methylation can be deposited on chromatin-associated RNAs (caRNAs) by the RNA methyltransferase complex (MTC) to regulate chromatin state and transcription. However, mechanism which MTC is recruited distinct genomic loci remains elusive. Here we identify RBFOX2, a well-studied RNA-binding protein, as factor that preferentially recognizes m6A caRNAs. RBFOX2 recruit RBM15, an component, facilitate of promoter-associated RNAs. RBM15 also physically interacts with YTHDC1...
Abstract METTL3 and METTL14 are two components that form the core heterodimer of main RNA m6A methyltransferase complex (MTC) installs m6A. Surprisingly, depletion or displayed distinct effects on stemness maintenance mouse embryonic stem cell (mESC). While comparable global hypo-methylation in was observed Mettl3 Mettl14 knockout mESCs, respectively. led to a globally decreased nascent synthesis, whereas resulted transcription upregulation, suggesting might possess an m6A-independent role...
Abstract Precise and reliable cell-specific gene delivery remains technically challenging. Here we report a splicing-based approach for controlling expression whereby separate translational reading frames are coupled to the inclusion or exclusion of mutated, frameshifting alternative exons. Candidate exons identified by analyzing thousands publicly available RNA sequencing datasets filtering cell specificity, conservation, local intron length. This method, which denote splicing-linked design...
Emergence of blaKPC and blaNDM co-producing Klebsiella pneumoniae strains have led to the limited therapeutic options for clinical treatment. Understanding diversity frequency resistance virulence genes these isolates is great significance.The aim this study research in strains.In study, 117 K. pneumonia were isolated from China, among which, 24 found be with significant against almost all commonly used antibiotics. Additionally, 4 hypermucoviscous 8 showed high serum resistance. Overall,...
Hexanucleotide repeat expansion in the
Many organisms alter the chromatin state of unsynapsed chromosomes during meiotic prophase, a phenomenon hypothesized to function in maintaining germline integrity. In Caenorhabditis elegans, histone H3 lysine 9 dimethylation (H3K9me2) is detected by immunolabeling as enriched on chromosomes. Loss SET domain protein, MET-2, greatly reduces H3K9me2 abundance and results mortality. Here, we used him-8 mutations disable X chromosome synapsis performed combination molecular assays map sites...
Cisplatin is a broad-spectrum anti-tumour drug commonly used in clinical practice. However, its ototoxicity greatly limits application, and no effective method available to prevent this effect. Endoplasmic reticulum stress (ERS) reportedly involved cisplatin ototoxicity, but the exact mechanism remains unclear. Therefore, study aimed investigate role of eukaryotic translation initiation factor 2α (eIF2α) signalling dephosphorylation inhibitor salubrinal ototoxicity. We evaluated whether...
The present study aimed to identify a specific circular RNA (circRNA) for early diagnosis of gastric cancer (GC).Totally 82 patients with GC, 30 chronic nonatrophic gastritis and atrophic were included in this study. Four the GC selected screening. Total from malignant adjacent tissue samples was extracted, circRNAs four screened. According screening results, eight most upregulated downregulated statistically significant association identified by real-time fluorescent quantitative polymerase...
Abstract Precise and reliable cell-specific gene delivery remains technically challenging. Here we report a splicing-based approach for controlling expression whereby separate translational reading frames are coupled to the inclusion or exclusion of alternative exons. Candidate exons identified by analyzing thousands publicly available RNA sequencing datasets filtering cell specificity, sequence conservation, local intron length. This method, which denote splicing-linked design (SLED), can...
Abstract Background : The present study attempted to detect a specific circular RNA (circRNA) for the early diagnosis of gastric cancer (GC). Methods: We selected four patients with GC this study. total malignant and adjacent tissues was extracted, circRNA screened. eight most upregulated downregulated circRNAs statistically significant relation paired nontumorous were identified using real-time fluorescent quantitative polymerase chain reaction (PCR). CircRNA expression by reverse...
Abstract Background: This study aimed to find the specific circular RNA (circRNA) for gastric cancer (GC) and lay foundation early diagnosis of GC. Methods: Four patients with GC were selected this study. The total their tissues adjacent was extracted, circRNA screened. top eight upregulated downregulated circRNAs statistical significance between paired nontumorous identified using real-time fluorescent quantitative polymerase chain reaction (PCR). expression level by reverse transcription...
Abstract Precise cell-specific gene delivery remains technically challenging. Here we report a splicing-based approach whereby separate translational reading frames are coupled to the inclusion or exclusion of alternative exons. Candidate exons identified by analyzing publicly available RNA sequencing datasets and filtering cell specificity, sequence conservation, intron length. This method, which term splicing-linked expression design (SLED), can be combined in Boolean manner with existing...
This study aimed to explore the structural and functional characteristics of fecal-associated microbiome (FAM) between phlegm-dampness constitution (PDC) balanced (BC), screen related specific operational taxonomic unit (OTU) biomarkers. was a cross-sectional study. After strictly identifying subjects, their clinical index recorded counted. Fecal samples were collected for 16S rDNA sequencing. Alpha diversity, beta relative abundance dominant bacterial taxa used describe FAM structure,...
Abstract Background: The present study aimed to identify a specific circular RNA (circRNA) for early diagnosis of gastric cancer (GC). Methods: Totally 82 patients with GC, 30 chronic nonatrophic gastritis and atrophic were included in this study. Four the GC selected screening. Total from malignant adjacent tissue samples was extracted, circRNAs four screened. According screening results, eight most upregulated downregulated statistically significant association identified by real-time...