A5007602365- Epigenetics and DNA Methylation
- Muscle Physiology and Disorders
- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Adipose Tissue and Metabolism
- Animal Genetics and Reproduction
- Muscle metabolism and nutrition
- Lipid metabolism and biosynthesis
- Cancer-related molecular mechanisms research
- Histone Deacetylase Inhibitors Research
- RNA modifications and cancer
- Genetics, Aging, and Longevity in Model Organisms
- Fatty Acid Research and Health
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Renal and related cancers
- Plant biochemistry and biosynthesis
- Ruminant Nutrition and Digestive Physiology
- Biofuel production and bioconversion
- Nutrition, Genetics, and Disease
- Ginseng Biological Effects and Applications
- Genetic diversity and population structure
- Congenital heart defects research
- stochastic dynamics and bifurcation
Inner Mongolia University
2016-2025
Nantong University
2011-2025
Affiliated Hospital of Nantong University
2011-2025
Third Hospital of Hebei Medical University
2017-2025
Hebei Medical University
2017-2025
Affiliated Hospital of Southwest Medical University
2022-2024
Southwest Medical University
2024
Shanghai East Hospital
2023
Zhejiang A & F University
2019-2022
Foundation Medicine (United States)
2022
Abstract METTL3 and METTL14 are two components that form the core heterodimer of main RNA m6A methyltransferase complex (MTC) installs m6A. Surprisingly, depletion or displayed distinct effects on stemness maintenance mouse embryonic stem cell (mESC). While comparable global hypo-methylation in was observed Mettl3 Mettl14 knockout mESCs, respectively. led to a globally decreased nascent synthesis, whereas resulted transcription upregulation, suggesting might possess an m6A-independent role...
Under subarachnoid hemorrhage (SAH) conditions, astrocytes undergo a marked intensification of glycolytic activity, resulting in the generation substantial amounts lactate to maintain energy demand for neurons and other brain cells. Lactate has garnered increasing attention recent years because its emerging role critical biological processes such as inflammation regulation neuroprotection, particularly through histone lactylation. Bromodomain-containing protein 4 (BRD4) plays crucial...
Chinese Yellow Cattle, an ancient and domesticated breed for draft service, provide unique animal genetic resources with excellent features, including crude feed tolerance, good stress resistance, strong adaptability, tender meat quality; however, their production performance yield are significantly inferior. Herein, the myostatin gene (MSTN), a negative regulator of skeletal muscle development, was knocked out by CRISPR/Cas9 technology. Eight MSTN gene-edited bull calves (MT) were born, six...
Polyunsaturated fatty acids (PUFAs) such as linoleic acid (18:2, n-6) and α-linolenic (18:3, n-3) are essential for the growth, development, well-being of mammals. However, most mammals, including humans, cannot synthesize n-3 n-6 PUFAs these must be obtained through diet. The beneficial effect converting polyunsaturated (n-6 PUFAs) into (n-3 has led to extensive research on flax desaturase 3 (Fad3) gene, which encodes desaturase. Still, plant-derived Fad3 gene is used much less in...
Myostatin (MSTN) is a protein that plays crucial role in regulating skeletal muscle development. Despite the known benefits of MSTN mutant cattle for increasing beef production, their potential impact on CH4 emissions has not been quantified. The study comparing wild-type (WT) to MSTN-knockout (MSTN-KO) revealed production was lower. Macrogenomic analysis significant decrease rumen archaea, with reduced Richness indices (P = 0.036). MSTN-KO also showed altered archaea distribution and...
N6-methyladenosine (m6A) modification is a key methylation involved in reproductive processes. Myostatin gene editing (MT) cattle known to enhance muscle mass and productivity. However, the changes m6A MT bull sperm remain poorly understood. In wild-type (WT) groups, we identified 25,542 22,253 peaks, respectively, mainly concentrated coding sequence (CDS) 3′ untranslated region (UTR) of genes. The group showed an increase transcription, but there was no significant difference overall peaks...
This study investigated the knowledge, attitude, and practice (KAP) of aesthetic medicine practitioners concerning laser and/or light therapy for hypertrophic scars. Conducted at Hebei Medical University Third Hospital from December 25, 2023, to January 7, 2024, cross-sectional utilized a self-administered questionnaire gather socio-demographic data KAP scores. A total 424 valid questionnaires were collected, with 220 (52.26%) female participants. The mean scores 27.24 ± 4.28 knowledge...
ABSTRACT The initiation and progression of clear cell renal carcinoma (ccRCC) are closely linked to significant metabolic alterations. Specifically, lipid metabolism alterations their association with the high invasiveness in ccRCC require further investigation. After conducting RNA‐sequencing (RNA‐seq), we discovered that Hydroxyacyl‐CoA Dehydrogenase Trifunctional Multienzyme Complex Subunit Beta ( HADHB ) was significantly downregulated highly invasive line. It found expression tumor...
Superovulation and embryo transfer are key technologies to improve the reproductive ability of female animals enhance efficiency livestock production. However, poor-quality oocytes or abnormal fluctuations hormone levels caused by superovulation affect embryonic development environment, which may lead a significant decline in number quality transferable embryos, thus reducing superovulation. In this study, nicotinamide adenine dinucleotide (NAD+) was injected into Huaxi cows during period...
<title>Abstract</title> Background Long breeding cycle, long calving intervals and typical single calves limit the potential for improving their economic benefits. Ensuring reproductive performance efficiency of cows are crucial to increasing value. Factors affecting include breed, pre-pregnancy maternal preparation, nutrition during pregnancy, perinatal management. The gene editing MSTN can improve development skeletal muscles provide a new way promotion existing beef cattle breeds....
During embryonic development, zygotic genome activation (ZGA) is a critical event that determines the rational process and fate of cells. The tricarboxylic acid cycle (TCA cycle) provides necessary reactants energy for biological activities such as activation, chromatin opening, epigenetic modifications during ZGA. Recent studies have shown ZGA, core enzymes associated with TCA briefly enter nucleus participate in initiating ZGA process. However, regulatory relationship between factors, Dux,...
Article2 November 2018Open Access Transparent process KDM6A and KDM6B play contrasting roles in nuclear transfer embryos revealed by MERVL reporter system Lei Yang orcid.org/0000-0002-9767-9026 State Key Laboratory of Reproductive Regulation Breeding Grassland Livestock, Inner Mongolia University, Hohhot, China Search for more papers this author Lishuang Song Research Center Mammalian Biology Biotechnology, College Life Sciences, Xuefei Liu Lige Bai Guangpeng Li Corresponding Author [email...
N6-methyladenosine (m6A) methylation is the most common and abundant modification on mammalian messenger RNA (mRNA) regulates pluripotency of embryonic stem cells (ESCs). Research has shown that melatonin plays a fundamental role in DNA histone modifications. However, effect unknown. Here, for first time, we investigated m6A modifications long-term-cultured ESCs. Pluripotency studies indicated 10 μmol/L sufficiently maintained ESCs with stemness features over 45 passages (more than 90 days)....
Myostatin (MSTN) is mostly expressed in skeletal muscle and plays crucial roles the negative regulation of mass development.The methylation demethylation myogenesis-specific genes are major regulatory factors satellite cell differentiation.The present study was designed to investigate mechanism myogenic differentiation regulated by MSTN mutation (MT) methylation/demethylation state downstream genes.The results showed that, -/+ cells, a higher myotube fusion index larger length were observed...
Drought is a misfortune for agriculture and human beings. The annual crop yield reduction caused by drought exceeds the sum of all pathogens. As one gatekeepers China's "granary", rice most important to reveal key tolerance factors in rice. Rice seedlings Nipponbare (Oryza sativa L. ssp. Japonica) were subjected simulated stress, their root systems analyzed non-targeted metabolome strand-specific transcriptome. We found that both DEGs metabolites enriched purine metabolism, allantoin...
Myostatin (MSTN), a growth and differentiation factor, plays an important role in regulating skeletal muscle development. MSTN knockout (MSTN-KO) leads to hypertrophy regulates metabolic homeostasis. Moreover, is also detected smooth muscle. However, the effect of MSTN-KO on has not yet been reported. In this study, combined metabolome transcriptome analyses were performed investigate transcriptional profiling esophageal muscles Chinese Luxi Yellow cattle (n = 5, 24 months, average body...
DNA methylation has been investigated for many years, but recent technologies have allowed single-cell- and single-base-resolution datasets more accurate assessment of dynamics at the key genomic regions that regulate gene expression in human early embryonic development. In this study, region from upstream 20 kb to downstream RefSeq was selected divided into 12 distinct (up20, up10, up5, up2, 5'UTR, exon, intron, 3'UTR, down2, down5, down10 down20). The candidate promoter (TSS ± 2 kb)...
Myostatin (Mstn) is a major negative regulator of skeletal muscle mass and initiates multiple metabolic changes. The deletion the Mstn gene in mice leads to reduced mitochondrial functions. However, underlying regulatory mechanisms remain unclear. In this study, we used CRISPR/Cas9 generate myostatin-knockout (Mstn-KO) via pronuclear microinjection. Mstn-KO exhibited significantly larger muscles. Meanwhile, knockout regulated organ weights mice. Moreover, found that basal rate, adenosine...
Myostatin (MSTN) is an important negative regulator of skeletal muscle growth in animals. A lack MSTN promotes lipolysis and glucose metabolism but inhibits oxidative phosphorylation (OXPHOS). Here, we aimed to investigate the possible mechanism regulating mitochondrial energy homeostasis muscle. To this end, knockout mice were generated by CRISPR/Cas9 technique. Expectedly, null (Mstn-/-) mouse has a hypermuscular phenotype. The Mstn-/- was detected enzyme-linked immunosorbent assay,...