A5100413276- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Tissue Engineering and Regenerative Medicine
- Tea Polyphenols and Effects
- Renal and related cancers
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Sperm and Testicular Function
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Virus-based gene therapy research
- Circular RNAs in diseases
- Oral microbiology and periodontitis research
- Reproductive System and Pregnancy
- Metabolism and Genetic Disorders
- Mycobacterium research and diagnosis
- Genetic Syndromes and Imprinting
- Glycosylation and Glycoproteins Research
- Hormonal Regulation and Hypertension
- Bone Metabolism and Diseases
Northeast Agricultural University
2016-2025
Hunan Agricultural University
2013-2025
Sun Yat-sen University
2012-2025
The First Affiliated Hospital, Sun Yat-sen University
2012-2025
Hunan Normal University
2012-2025
Ministry of Agriculture and Rural Affairs
2020-2025
Hefei National Center for Physical Sciences at Nanoscale
2023-2025
University of Science and Technology of China
2013-2025
Nanjing Drum Tower Hospital
2018-2025
Zhongshan Hospital of Xiamen University
2024-2025
Hyperacute rejection of porcine organs by old world primate recipients is mediated through preformed antibodies against galactosyl-α-1,3-galactose (Galα-1,3-Gal) epitopes expressed on the pig cell surface. Previously, we generated inbred miniature swine with a null allele α-1,3-galactosyltransferase locus ( GGTA1 ) nuclear transfer (NT) gene-targeted fibroblasts. To expedite generation pigs, selected spontaneous mutant cells from fibroblast cultures heterozygous animals for use in another...
Lipid droplets are the main lipid storage sites in cells. droplet homeostasis is regulated by surface accessibility of lipases. Mammalian adipose triglyceride lipase (ATGL) and hormone-sensitive (HSL) two key lipases for basal stimulated lipolysis, respectively. Perilipins, best known proteins, can either recruit or prevent access to droplets. Mammals have five perilipin which often exhibit redundant functions, precluding analysis exact role individual perilipins vivo. Drosophila only...
Berardinelli-Seip congenital lipodystrophy 2 (BSCL2) is caused by loss-of-function mutations in SEIPIN, a protein implicated both adipogenesis and lipid droplet expansion but whose molecular function remains obscure. Here, we identify physical functional interactions between SEIPIN microsomal isoforms of glycerol-3-phosphate acyltransferase (GPAT) multiple organisms. Compared to controls, GPAT activity was elevated SEIPIN-deficient cells tissues kinetic values were altered. Increased appears...
The inflammasomes are signaling platforms that promote the activation of inflammatory caspases such as caspases-1, -4, -5, and -11. Recent studies identified gasdermin D (GSDMD) an effector for pyroptosis downstream inflammasome pathways. Cleavage GSDMD by allows its N-terminal domain to associate with membrane lipids form pores induce pyroptotic cell death. Despite important role in pyroptosis, molecular mechanisms recognition cleavage trigger poorly understood. Here, we demonstrate...
Obesity is characterized by accumulation of excess body fat, while lipodystrophy loss or absence fat. Despite their opposite phenotypes, these two conditions both cause ectopic lipid storage in non-adipose tissues, leading to lipotoxicity, which has health-threatening consequences. The exact mechanisms underlying remain elusive. Here we report the analysis a Drosophila model most severe form human lipodystrophy, Berardinelli-Seip Congenital Lipodystrophy 2, caused mutations BSCL2/Seipin...
Abstract Oleic acid (OA), a main ingredient of Brucea javanica oil (BJO), is widely known to have anticancer effects in many tumors. In this study, we investigated the effect OA and its mechanism tongue squamous cell carcinoma (TSCC). We found that effectively inhibited TSCC proliferation dose- time-dependent manner. treatment significantly induced cycle G0/G1 arrest, increased proportion apoptotic cells, decreased expression CyclinD1 Bcl-2, p53 cleaved caspase-3. also obviously formation...
Because few studies exist to describe the unique molecular network regulation behind pig pre-implantation embryonic development (PED), genetic engineering in embryo is limited. Also, this lack of research has hindered derivation and application porcine stem cells induced pluripotent (iPSCs).We identified analyzed genome wide transcriptomes vivo-derived somatic cell nuclear transferred (SCNT) as well mouse embryos at different stages using mRNA deep sequencing. Comparison with those human...
Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model development livestock breeding. Despite ongoing attempts since the 1990s, no stably defined pig cell line has been established. Here, guided by insights from a large-scale single-cell transcriptome analysis of embryos embryonic day (E) 0 E14, specifically, tracing pluripotency changes during epiblast development, we developed an in vitro culture medium...
Metabolism feeds into the regulation of epigenetics via metabolic enzymes and metabolites. However, features, their impact on epigenetic remodeling during mammalian pre-implantation development, remain poorly understood. In this study, we established landscape mouse embryos from zygote to blastocyst, quantified some absolute carbohydrate We integrated these data with transcriptomic proteomic data, discovered characteristics development process, including activation methionine cycle 8-cell...
SUMMARY Anthocyanin and catechin production in tea ( Camellia sinensis ) leaves can positively affect quality; however, their regulatory mechanisms are not fully understood. Here we report that, while the CsMYB75‐ or CsMYB86‐directed MYB–bHLH–WD40 (MBW) complexes differentially activate anthocyanin biosynthesis leaves, respectively, CsMYBL2a CsMYBL2b homologs negatively modified light‐ temperature‐induced both Arabidopsis plants. The MBW activated synthesis genes downstream repressor ....
Abstract Osteoarthritis (OA) is the most common degenerative joint disorder that causes disability in aged individuals, caused by functional and structural alterations of knee joint. To investigate whether metabolic drivers might be harnessed to promote cartilage repair, a liquid chromatography–mass spectrometry (LC–MS) untargeted metabolomics approach was carried out screen serum biomarkers osteoarthritic rats. Based on correlation analyses, α-ketoglutarate (α-KG) has been demonstrated have...
Journal Article Virus Burden in Long-Term Survivors of Human Immunodeficiency (HIV) Infection Is a Determinant Anti-HIV CD8+ Lymphocyte Activity Get access John Ferbas, Ferbas Departments Medicine and Microbiology Immunology, School Medicine, Department Epidemiology, Public Health, University California at Los Angeles; Therion Biologics Corporation, Cambridge, Massachusetts Search for other works by this author on: Oxford Academic PubMed Google Scholar Andrew H. Kaplan, Kaplan Mary Ann...
Somatic cell nuclei of giant pandas can dedifferentiate in enucleated rabbit ooplasm, and the reconstructed eggs develop to blastocysts. In order observe whether these interspecies cloned embryos implant uterus an animal other than panda, we transferred approximately 2300 panda-rabbit into 100 synchronized recipients, none became pregnant. another approach, cotransferred both cat-rabbit oviducts 21 cat recipients. Fourteen recipients exhibited estrus within 35 days; five 43–48 days after...