A5060560991- RNA modifications and cancer
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- MicroRNA in disease regulation
- Renal and related cancers
- Viral Infectious Diseases and Gene Expression in Insects
- Metabolism and Genetic Disorders
- Genetics and Neurodevelopmental Disorders
- Neonatal Respiratory Health Research
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
Wenzhou Medical University
2022-2025
Northeast Agricultural University
2020-2022
Metabolism feeds into the regulation of epigenetics via metabolic enzymes and metabolites. However, features, their impact on epigenetic remodeling during mammalian pre-implantation development, remain poorly understood. In this study, we established landscape mouse embryos from zygote to blastocyst, quantified some absolute carbohydrate We integrated these data with transcriptomic proteomic data, discovered characteristics development process, including activation methionine cycle 8-cell...
Lactate is present at a high level in the microenvironment of mammalian preimplantation embryos vivo and vitro. However, its role development unclear. Here, we report that lactate highly enriched nuclei early when major zygotic genome activation (ZGA) occurs humans mice. The inhibition production uptake results developmental arrest 2-cell stage, ZGA failure, loss lactate-derived H3K18lac, which could be rescued by addition Lac-CoA recapitulated overexpression H3K18R mutation. By profiling...
Cell metabolism plays a crucial role in regulating the pluripotency of human embryonic stem cells (hESCs). Chlorogenic acid (CGA), an essential dietary polyphenol, exhibits diverse pharmacological effects on regulation. This study examines CGA cell hESCs using H9 model. At concentration 100 µg/ml, showed low toxicity and had no impact viability cells. Furthermore, it promotes NANOG expression. Importantly, enhances Fatty β-oxidation (FAO), thus promoting proliferation lipid synthesis...
Abstract In mammalian early embryos, the transition from maternal to embryonic control of gene expression requires timely degradation a subset mRNAs (MRD). Recently, zygotic genome activation (ZGA)-dependent MRD has been characterized in mouse 2-cell embryo. However, dynamics is still poorly understood, and factor-mediated before along with ZGA not investigated. Argonaute 2 ( Ago2 ) highly expressed oocyte embryos. this study, we showed that -dependent involving RNA interference (RNAi)...
Long non-coding RNAs (lncRNAs) have been implicated in diverse biological processes. However, the functional mechanisms not yet fully explored. Characterizing interactions of lncRNAs with chromatin is central to determining their functions but, due precise and efficient approaches lacking, our understanding has progressed slowly. In this study, we demonstrate that a nuclear lncRNA linc1393 maintains mouse ESC pluripotency by recruiting SET1A near its binding sites, establish H3K4me3 status...
Long noncoding RNAs (lncRNAs) are increasingly being recognized as modulators in various biological processes. However, due to their low expression, systematic characterization is difficult determine. Here, we performed transcript annotation by a newly developed computational pipeline, termed RNA-seq and small combined strategy (RSCS), wide variety of cellular contexts. Thousands high-confidence potential novel transcripts were identified the RSCS, reliability transcriptome was verified...
Abstract Metabolism feeds into the regulation of epigenetics via metabolic enzymes and metabolites. However, features, their impact on epigenetic remodeling during mammalian pre-implantation development, remain poorly understood. In this study, we established landscape mouse embryos from zygote to blastocyst, quantified some absolute carbohydrate We integrated these data with transcriptomic proteomic data, discovered characteristics development process, including activation methionine cycle...