A5101810948- CRISPR and Genetic Engineering
- Aluminum Alloys Composites Properties
- Microstructure and mechanical properties
- Aluminum Alloy Microstructure Properties
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Renal and related cancers
- Tissue Engineering and Regenerative Medicine
- Metal Alloys Wear and Properties
- Microstructure and Mechanical Properties of Steels
- Xenotransplantation and immune response
- Advanced materials and composites
- Single-cell and spatial transcriptomics
- Photorefractive and Nonlinear Optics
- Crystal Structures and Properties
- Metal and Thin Film Mechanics
- Virus-based gene therapy research
- Plant Genetic and Mutation Studies
- MXene and MAX Phase Materials
- Mesenchymal stem cell research
- Circular RNAs in diseases
- Chalcogenide Semiconductor Thin Films
- Extraction and Separation Processes
North University of China
2021-2024
Northeast Agricultural University
2008-2024
Nanjing University of Finance and Economics
2022
Guangxi University
2013-2018
Ningbo University of Technology
2013
North East University
2008
Although the pig is considered an important model of human disease and ideal animal for preclinical testing cell transplantation, utility this has been hampered by a lack genuine porcine embryonic stem cells. Here, we derived pluripotent (pPSC) line from day 5.5 blastocysts in newly developed culture system based on MXV medium 5% oxygen atmosphere. The pPSCs had passaged more than 75 times over two years, morphology colony was similar to that Characterization assessment showed were alkaline...
N-ethyl-N-nitrosourea (ENU) mutagenesis is a powerful tool to generate mutants on large scale efficiently, and discover genes with novel functions at the whole-genome level in Caenorhabditis elegans, flies, zebrafish mice, but it has never been tried model animals. We describe successful systematic three-generation ENU screening pigs establishment of Chinese Swine Mutagenesis Consortium. A total 6,770 G1 6,800 G3 were screened, 36 dominant 91 recessive pig families various phenotypes...
Nanostructured bainite is extremely resistant to severe tempering making it difficult reduce its hardness sufficiently allow certain processing before heat treatment into final state. An attempt therefore made introduce the divorced pearlite reaction that relies on presence of pro-eutectoid cementite in austenite order produce a microstructure containing spheroidal particles simply by continuous cooling from two phase and field. It demonstrated dramatically reduced values can be achieved...
To investigate the effects of tannins (TA) on porcine oocyte in vitro maturation (IVM), different concentrations TA (0, 1, 10 and 100 μg/mL) were supplemented with a medium COCs subsequent embryonic development examined. The results showed that µg/mL significantly improved cumulus expansion index (CEI), cumulus-expansion-related genes (PTGS1, PTGS2, PTX-3, TNFAIP6 HAS2) expression blastocyst formation rates after parthenogenetic activation (PA), fertilization (IVF) somatic cell nuclear...
In vitro-fertilized (IVF) and parthenogenetically activated (PA) embryos, key to genetic engineering, face more developmental challenges than in vivo-developed embryos (IVV). We analyzed single-cell RNA-seq data from the oocyte eight-cell stages IVV, IVF, PA porcine focusing on differences during early zygotic genome activation (ZGA), a vital stage for embryonic development. (1) Our findings reveal that vitro (IVF PA) exhibit similar trajectories compared IVV with showing least gene...
Abstract In mammalian early embryos, the transition from maternal to embryonic control of gene expression requires timely degradation a subset mRNAs (MRD). Recently, zygotic genome activation (ZGA)-dependent MRD has been characterized in mouse 2-cell embryo. However, dynamics is still poorly understood, and factor-mediated before along with ZGA not investigated. Argonaute 2 ( Ago2 ) highly expressed oocyte embryos. this study, we showed that -dependent involving RNA interference (RNAi)...
By adding Sn and Sc alone or in combination, as well conducting a tensile test at 573 K, the synergistic effect of + on high-temperature strengthening Al–5Cu heat-resistant alloy was investigated. Transmission electron microscope, elements mapping first-principle calculations were used to characterise microstructure evolution precipitation particles various states uncover processes for strengthening. It has been discovered that Sc, either individually together, can significantly improve...
High lead-tin bronze is widely used in the selection of wear-resistant parts such as bearings, bearing bushes, aerospace pump rotors, turbines, and guide plates because its excellent wear resistance, thermal conductivity, fatigue strong load-bearing capacity. At present, high a material for bimetal cylinders, which cannot meet requirements high-strength, anti-wear actual working conditions under temperature, speed, heavy load conditions, prone to de-cylinder, cylinder holding, copper...
Abstract Although we have obtained porcine pluripotent stem cell lines (pPSCs) from blastocysts, the cells exhibit flat clonal morphology and do not support single‐cell passage. There is massive death after dissociation, efficiency of colony generally ≤10%. In a recent study, got new pPSCs using two Wnt signaling pathway regulators CHIR99021 XAV939. This had strong biological viability, small‐domed morphology, its cloning dissociation was 80–90%. The CH/XAV‐treated expressed elevated levels...
Upregulation of Cdx2 expression in outer cells is a key event responsible for cell lineage segregation between the inner mass and trophoderm (TE) mouse morula‐stage embryos. In TE cells, polarization can regulate Hippo Rho‐associated kinase (Rho‐ROCK) signaling to induce nuclear location YAP, which has been demonstrated further . However, we found that CDX2 could not be detected porcine embryos but only some at early blastocyst stage. The biological significance regulation mechanism this...
Abstract Drought stress has been a key environmental factor affecting plant growth and development. The genome is capable of producing long noncoding RNAs (lncRNAs). To better understand white mulberry ( Morus alba L.) drought response mechanism, we conducted comparative transcriptome study comparing two treatments: drought‐stressed (EG) well‐watered (CK) plants. A total 674 differentially expressed lncRNAs (DElncRNAs) were identified. In addition, 782 messenger (DEmRNAs) We Gene Ontology...
The aim of this study was to verify whether small molecules can improve the efficiency precision gene editing using clustered regularly interspaced short palindromic repeats (CRISPR) ribonucleoprotein (RNP) in porcine cells. CRISPR associated 9 (Cas9) protein, guide RNA (sgRNA), phosphorothioate-modified single-stranded oligonucleotides (ssODN), and different were used generate precise nucleotide substitutions at insulin (INS) by homology-directed repair (HDR) fetal fibroblasts (PFFs). These...
The structural parameter, elastic anisotropy, and thermal properties of cubic MgIn2S4 under pressures is examined using first principles techniques. determined lattice constant exhibits a high level concurrence with the values reported in existing literature. Based on mechanical characteristics, there an observed enhancement both ductility anisotropy increased pressure. investigation focuses encompassing impact temperature pressure key parameters such as Debye temperature, Grüneisen et al.
The detailed mechanism of divorced pearlite in bearing steels began to be actively discussed more recently. survey higher Mn steel was carried out through scanning electronic microscope following isothermal transformation heat treatment. results show that while taking place the just slightly below A1, cementite could produced such way as growing on base pre-existing particles austenite, or emerge either globular short rod directly from austenite. Under transformation, carbon atoms austenite...
The property of anti-penetration plays a leading role in modern composite target plate. In order to research the mechanism anti-penetration, 12.7mm wadcutter was used penetrate an AD95 Ceramic / Metal ANSYS LS-DYNA non-linear finite element analysis software applied establish and mesh model based on 3D Lagrange equation. A comparison made between experimental numerical analyzing results. design is given by adding constrained steel plate best thickness maximize anti-penetration. provides...