A5036560647- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- Effects of Radiation Exposure
- Cancer Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Regulation of Appetite and Obesity
- Mosquito-borne diseases and control
- Adipokines, Inflammation, and Metabolic Diseases
- Viral gastroenteritis research and epidemiology
- Xenotransplantation and immune response
- Biochemical effects in animals
- Virology and Viral Diseases
- RNA regulation and disease
- Viral Infections and Vectors
- Botanical Research and Chemistry
- Animal Virus Infections Studies
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- Enzyme Structure and Function
- Cancer Cells and Metastasis
- Cardiac Ischemia and Reperfusion
- Kruppel-like factors research
- Radiation Therapy and Dosimetry
- Cardiac Fibrosis and Remodeling
Wuyi University
2023-2025
Beike Biotechnology (China)
2024
Southern Medical University
2012-2023
Hangzhou Center for Disease Control and Prevention
2023
Wuyi University
2019-2022
Songshan Lake Materials Laboratory
2014-2021
Jilin Agricultural University
2017
Pearl River Hydraulic Research Institute
2017
Beijing Normal University
2013-2015
University of Tennessee Health Science Center
2014
Neurodegeneration is a hallmark of many neurological diseases, including Alzheimer's, Parkinson's and the polyglutamine which are all caused by misfolded proteins that accumulate in neuronal cells brain. Although apoptosis believed to contribute neurodegeneration these cases, genetic mouse models diseases often fail replicate overt Using nuclear transfer, we generated transgenic Huntington's disease (HD) pigs express N-terminal (208 amino acids) mutant huntingtin with an expanded tract...
Multiple genetic modifications in pigs can essentially benefit research on agriculture, human disease and xenotransplantation. Most multi-transgenic have been produced by complex time-consuming breeding programs using multiple single-transgenic pigs. This study explored the feasibility of producing viral 2A peptide light previous indicating that it be utilized for multi-gene transfer gene therapy somatic cell reprogramming. A peptide-based double-promoter expression vector mediated four...
The transcription factor Krüppel-like 4 (KLF4) has been implicated in regulating cell proliferation, migration and differentiation a variety of human cells is one four factors required for the induction pluripotent stem reprogramming. However, its role not addressed ocular neovascular diseases. This study investigated KLF4 angiogenesis underlying molecular mechanisms retinal microvascular endothelial (HRMECs). functional HRMECs was determined following lentiviral vector mediated inducible...
Abstract Background Makorin ring finger protein 3 gene ( MKRN3 ) mutation is the most common genetic cause of central precocious puberty (CPP) in children. Due to lack ideal ‐modified animal model mice enter only 4–5 days earlier than normal mice), related research limited. Methods Therefore, rabbit was developed using CRISPR (clustered regularly interspaced short palindromic repeats) editing technology. The genotype identification and phenotype evaluation rabbits were carried out. Results...
Ovarian cancer presents therapeutic challenges due to its typically late detection, aggressive metastasis, and resistance. The transcription factor Krüppel-like 4 (KLF4) has been implicated in human cancers as a tumor suppressor or oncogene, although role depends greatly on the cellular context. of KLF4 ovarian not elucidated mechanistic detail. In this study, we investigated cells by transducing cell lines SKOV3 OVCAR3 with doxycycline-inducible lentiviral vector. Overexpression reduced...
Clinical evidence suggests that there exists a strong correlation between Zika virus (ZIKV) infection and abnormal development of the nervous system.However, underlying mechanisms remain to be elusive.In this study, recombinant lentiviral vectors coding for ZIKV structural proteins truncations (prM-Env, M-Env Env) were transduced into PC12 cells.Envelope (Env) overexpression induced significant inhibition proliferation triggered G2/M cell cycle arrest apoptosis in cells.Flow cytometry...
In previous studies from other labs it has been well demonstrated that the ectopic expression of c-Myc in mammary epithelial cells can induce epithelial-mesenchymal transition (EMT), whereas our pilot experiment, epithelial-like morphological changes were unexpectedly observed c-Myc-expressing pig fibroblasts [i.e., porcine embryonic (PEFs) and dermal (PDFs)] mesenchymal stem cells, suggesting same gene is entitled to trigger EMT mesenchymal-epithelial (MET) fibroblasts. This prompted us...
miRNA biogenesis enzyme Drosha cleaves double-stranded primary by interacting with RNA binding protein DGCR8 and processes into precursor to participate in the pathway. The role of vascular smooth muscle cells (VSMCs) has not been well addressed. We generated conditional knockout (cKO) mice crossing VSMC-specific Cre mice, SM22-Cre, (loxp/loxp) mice. Disruption VSMCs resulted embryonic lethality at E14.5 severe liver hemorrhage mutant embryos. No obvious developmental delay was observed cKO...
N-ethyl-N-nitrosourea (ENU) mutagenesis is a powerful tool to generate mutants on large scale efficiently, and discover genes with novel functions at the whole-genome level in Caenorhabditis elegans, flies, zebrafish mice, but it has never been tried model animals. We describe successful systematic three-generation ENU screening pigs establishment of Chinese Swine Mutagenesis Consortium. A total 6,770 G1 6,800 G3 were screened, 36 dominant 91 recessive pig families various phenotypes...
Patients with heart failure (HF) have high mortality and mobility. Xinmailong (XML) injection, a Chinese Medicine, is clinically effective in treating HF. However, the mechanism of XML's effectiveness on HF was unclear, thus, target present study. We created mouse model pressure-overload-induced transverse aortic constriction (TAC) surgery compared among 4 study groups: SHAM (n = 10), TAC 12), MET (metoprolol, positive drug treatment, n 7) XML (XML 14). Dynamic changes cardiac structure...
Porcine pluripotent cells with the capacity to generate germ line chimeras have not been developed yet. The transcription factor Oct-4 is an important marker of undifferentiating status and a central regulator pluripotency in cells. Establishment promoter-based reporter system, such as that used mice, will be useful tool for monitoring differentiating statuses porcine both vivo vitro. In present study, we constructed vector, pOGN2, which enhanced green fluorescent protein (EGFP) was driven...
Heart failure(HF) is a dangerous disease that affects millions of patients. Radix Salvia widely used in Chinese clinics to treat heart diseases. Salvianolic acid B(SalB) the major active component Salvia. This study investigated mechanisms action and effects SalB on HF an experimental mouse model HF.We created by inducing pressure overload with transverse aortic constriction(TAC) surgery for 2 weeks compared among 4 groups: SHAM group (n = 10), TAC 9), TAC+MET (metprolol, positive drug...
The myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interesting candidate to improve disease in farm animals. In this study, we report the use somatic cell nuclear transfer (SCNT) produce transgenic pigs over-expressing Mx1 gene. These express approximately 15-25 times more mRNA than non-transgenic pigs, and protein level was also markedly enhanced. We challenged fibroblast cells isolated from ear skin control with influenza A virus classical...
Background The radiation-induced energy metabolism dysfunction related to injury and radiation doses is largely elusive. purpose of this study investigate the early response in small intestinal tissue its correlation with pathologic lesion after total body X-ray irradiation (TBI) Tibet minipigs. Methods Results 30 minipigs were assigned into 6 groups including 5 experimental one control group animals each group. these subjected a TBI 2, 5, 8, 11, 14 Gy, respectively. Small intestine tissues...
Rotavirus-related diarrhoea is considered one of the most important diseases in field animal production. In addition to classic vaccine strategy, a number studies have utilised probiotics, such as Lactobacillus rhamnosus GG (LGG), prevent rotavirus-induced diarrhoea. Although it has been partially revealed that Toll-like receptors (TLRs) are involved LGG-mediated protection against rotavirus infection, details underlying immunologic mechanisms remain largely unknown. this study,...
The effects of land use practices on soil fauna, especially earthworms, are poorly known in coastal saline agricultural soils. Here we compare earthworm communities six types practice the region China's Bohai Bay, namely uncultivated soil, two orchard (pear and winter jujube) lands, man-made forests (chinese ash), vegetable cropped (maize). In addition, recorded selected physicochemical properties soil. Soil organic matter content total N were significantly higher under pear than other...
FaeG is the major subunit of K88 fimbriae. These cell surface attachments are considered to be virulence factor enterotoxigenic Escherichia coli (ETEC), which causes diarrhoea in piglets. The use dendritic cell-targeting peptide (DCpep) has been demonstrated an effective approach enhance immunity vaccines. Lactobacillus plantarum attractive candidate for oral vaccination owing its beneficial effects and safety. In this study, L. was employed deliver a FaeG-DCpep fusion antigen, immune...
Hereditary tyrosinemia type I (HT1) results from the loss of fumarylacetoacetate hydrolase (FAH) activity and can lead to lethal liver injury (LLI). Therapeutic options for HT1 remain limited. The FAH−/− pig, a well-characterized animal model HT1, represents promising candidate testing novel therapeutic approaches treat this condition. Here, we report an improved single-step method establish biallelic (FAH−/−) mutant porcine using CRISPR-Cas9 cytoplasmic microinjection. We also tested...
Abstract Unexpectedly, we found that c-Myc-expressing porcine embryonic fibroblasts (PEFs) subcutaneously implanted into nude mice formed cartilage-like tissues in vivo, while previous studies revealed the direct conversion of mouse and human somatic cells chondrocytes by combined use several defined factors, including c-Myc, which prompted us to explore whether PEFs can be reprogrammed become pig induced chondrocyte-like (piCLCs) via ectopic expression c-Myc alone. In this study, PEFs,...
Increased expression of angiotensin II type 2 receptor (AT2R) induces apoptosis in numerous tumor cell lines, with either Angiotensin II-dependent or II-independent regulation, but its molecular mechanism remains poorly understood. Here, we used PCR Array analysis to determine the gene and microRNA profiles human prostate cancer lines transduced AT2R recombinant adenovirus. Our results demonstrated that over leads up-regulation 6 apoptosis-related genes (TRAIL-R2, BAG3, BNIPI, HRK, Gadd45a,...
Gene therapy's entrance into clinical settings has made it an ever more attractive field of study for various diseases. However, relatively little progress been in targeting kidney diseases due to poor gene delivery efficiency renal cells. The development novel therapy vectors medical intervention treat is needed. In this study, we designed and produced a pseudotyped lentiviral vector with envelope glycoproteins Zika virus (ZIKV), evaluated its potential use viral entry, neutralization...
The renin-angiotensin system (RAS) plays important roles in tumorigenesis and is involved with several hallmarks of cancer.Evidence shows that angiotensin II (AngII) type 1 receptor (AT1R) blockers may be associated improved outcome prostate cancer patients.Furthermore, our previous studies indicate increased expression Ang 2 (AT2R) alone induced apoptosis human lines, an effect did not require II.This study aimed to investigate the effects AT2R on tumor growth vivo we hypothesized...
Abstract Intramuscular fat (IMF) content has been generally recognized as a desirable trait in pork meat because of its positive effect on eating quality. An effective approach to enhance IMF is the generation transgenic pigs. In this study, we used somatic cell nuclear transfer (SCNT) generate cloned pigs exhibiting ectopic expression phosphoenolpyruvate carboxykinase (PEPCK-C) driven by an α-skeletal-actin gene promoter, which was specifically expressed skeletal muscle. Using qRT-PCR and...