A5065485631- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Sphingolipid Metabolism and Signaling
- Microtubule and mitosis dynamics
- Cancer Treatment and Pharmacology
- RNA modifications and cancer
- Metal-Organic Frameworks: Synthesis and Applications
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- Endoplasmic Reticulum Stress and Disease
- Cancer, Hypoxia, and Metabolism
- Magnetism in coordination complexes
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- X-ray Diffraction in Crystallography
- Cell death mechanisms and regulation
- Crystallization and Solubility Studies
- Cancer-related Molecular Pathways
- ATP Synthase and ATPases Research
- Kruppel-like factors research
University of Tennessee Health Science Center
2016-2025
Center for Cancer Research
2016-2025
Shandong Normal University
2023
University of Tennessee at Knoxville
2000-2015
Zhengzhou University
2009-2014
University of Pittsburgh
2006-2010
Princeton University
2010
Magee-Womens Hospital
2006-2007
Magee-Womens Research Institute
2007
MicroRNAs (miRNAs) are a recently discovered class of endogenous, small, noncoding RNAs that regulate about 30% the encoding genes human genome. However, role miRNAs in vascular disease is currently completely unknown. Using microarray analysis, we demonstrated for first time aberrantly expressed walls after balloon injury. The were further confirmed by Northern blot and quantitative real-time polymerase chain reaction. Modulating an overexpressed miRNA, miR-21, via antisense-mediated...
Neuroinflammation is implicated in the development and progression of many neurodegenerative diseases. Conditions that lead to a peripheral immune response are often associated with inflammation central nervous system (CNS), suggesting communication between neuroimmune system. The underlying mechanism this relationship remains largely unknown; however, experimental studies have demonstrated exposure infectious stimuli, such as lipopolysaccharide (LPS) or high-fat diet (HFD) feeding, result...
MicroRNA-21 (miR-21) is overexpressed in many human tumors and has been linked to various cellular processes altered cancer. miR-21 also up-regulated by a number of inflammatory agents, including IFN, which particular interest considering the close relationship between inflammation Because appears be melanoma, we examined role cancer development metastasis B16 mouse melanoma cells. We found that member an IFN-induced miRNA subset requires STAT3 activation. To characterize behavior,...
Abstract The microRNA miR-21 is overexpressed in many human cancers, wherein accumulating evidence indicates that it functions as an oncogene. Here, we report the cytokine IFN rapidly induces expression and mouse cells. Signal transducer activator of transcription 3 (STAT3) was implicated this pathway based on lack effect prostate cancer cells with a deletion STAT3 gene. ablation abrogated induction miR-21, confirming important role regulating miR-21. Chromatin immunoprecipitation analysis...
Despite advances in surgery, imaging, chemotherapy, and radiation, patients with glioblastoma multiforme (GBM), the most common histological subtype of glioma, have an especially dismal prognosis; >70% GBM die within 2 years diagnosis. In many human cancers, microRNA miR-21 is overexpressed, accumulating evidence indicates that it functions as oncogene. Here, we report overexpressed cell lines tumor tissue. Moreover, expression patient samples inversely correlated survival. Knockdown cells...
EF24 is a curcumin analog that has improved anticancer activity over curcumin, but its therapeutic potential and mechanism of action unknown, which important to address as targets multiple signaling pathways. inhibits the NF-κB not JAK-STAT pathway in DU145 human prostate cancer cells B16 murine melanoma cells. induces apoptosis these apparently by inhibiting miR-21 expression, also enhances expression several target genes, PTEN PDCD4. treatment significantly suppressed growth xenografts...
High-grade endometrioid and serous endometrial cancers (ECs) are an aggressive subtype of ECs without effective therapies. The reciprocal communication between tumor cells their surrounding microenvironment drives progression. Long noncoding RNAs (lncRNAs) key mediators tumorigenesis metastasis. However, little is known about the role lncRNAs in EC progression remodeling. We performed array-based lncRNA analysis a parental HEC-50 cell population derivatives with highly invasive,...
In ovarian cancer (OC) cells, Snail was reported to induce the epithelial-to-mesenchymal transition (EMT), which is a critical step in OC metastasis. At present little known about controlling expression cells by using specific microRNAs (miRNAs). We first used computational target prediction analysis identify 6 candidate miRNAs that bind 3′-untranslated region (3′-UTR) of mRNA. Among these miRNAs, two (miR-137 and miR-34a) with potential regulate were validated quantitative real-time PCR,...
PD-L1, a key inhibitory immune receptor, has crucial functions in cancer evasion, but whether PD-L1 promotes the malignant properties of cervical (CC) cells and mechanism by which is regulated CC remains unclear. We report that overexpressed CC, shRNA-mediated depletion suppresses proliferation, invasion, tumorigenesis cells. Loss miR-140/142/340/383 contributes to upregulation. miR-18a enhances levels targeting PTEN, WNK2 (ERK1/2 pathway inhibitor), SOX6 (Wnt/β-catenin inhibitor p53...
CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats) mediated genome editing is a powerful approach for loss of function studies. Here we report that lentiviral vectors are highly efficient in introducing mutations the precursor miRNA sequence, thus leading to expression and function. We constructed four different target regions miR-21 sequence found these gRNA induced sequences as shown by DNA surveyor mutation assay Sanger sequencing. Two were selected probe ovarian...
The microRNA miR-21 is overexpressed in most human cancers and accumulating evidence indicates that it functions as an oncogene. Since miRNAs suppress the expression of their target genes, we hypothesized some targets may act tumor suppressors, thus would be anticipated to reduced by high levels observed various cancers. By microarray analysis quantitative PCR identified validated FBXO11 (a member F-box subfamily lacking a distinct unifying domain) gene. component SKP1-CUL1-F-box ubiquitin...
Epithelial-to-mesenchymal transition (EMT) and aerobic glycolysis are fundamental processes implicated in cancer metastasis. Although increasing evidence demonstrates an association between EMT induction enhanced human cancer, the mechanisms linking these two conditions endometrial (EC) cells remain poorly defined.We characterized role molecular mechanism of glycolytic enzyme hexokinase 2 (HK2) mediating investigated how long noncoding RNA DLEU2 contributes to stimulation via upregulation...
20-hydroxyvitamin D 2 [20(OH)D ] inhibits DNA synthesis in epidermal keratinocytes, melanocytes, and melanoma cells a dose- time-dependent manner. This inhibition is dependent on cell type, with keratinocytes being more sensitive than normal melanocytes. The antiproliferative activity of 20(OH)D similar to that 1,25(OH) 3 newly synthesized 1,20(OH) but significantly higher 25(OH)D . also displays tumorostatic effects. In expression cyclins stimulates involucrin expression. It CYP24...
Autotaxin (ENPP2/ATX) and lysophosphatidic acid (LPA) receptors represent two key players in regulating cancer progression. The present study sought to understand the mechanistic role of LPA G protein-coupled (GPCR), not only tumor cells but also stromal microenvironment. B16F10 melanoma predominantly express LPA5 LPA2 lack LPA1. dose dependently inhibited invasion across a Matrigel layer. RNAi-mediated knockdown relieved inhibitory effect on without affecting basal invasion. This suggests...
Epithelial–mesenchymal transition (EMT) and dysregulated microRNAs (miRNAs) have important roles in driving chemoresistance. We previously reported that iASPP is a key EMT inducer could increase cisplatin resistance cervical cancer (CC) cells. Herein, we investigate the downstream mechanisms through which contributes to CC. By using lentiviral system, investigated effects of knockdown on CC cell growth chemosensitivity cells vivo. examined if miR-20a, was up-regulated following...
Due to peritoneal metastasis and frequent recurrence, ovarian cancer has the highest mortality among gynecological cancers.Epithelial mesenchymal transition (EMT) contributes tumor metastasis.In this study, we report for first time that metal regulatory transcription factor 1 (MTF1) was upregulated in cancer, its high expression associated with poor patient survival disease relapse.Knockout of MTF1 using lentiviral CRISPR/Cas9 nickase vector-mediated gene editing inhibited EMT by...