A5017910067- Prostate Cancer Treatment and Research
- Pancreatic and Hepatic Oncology Research
- Cancer-related Molecular Pathways
- Histone Deacetylase Inhibitors Research
- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- Cancer Mechanisms and Therapy
- Estrogen and related hormone effects
- Advanced Breast Cancer Therapies
- Cancer Treatment and Pharmacology
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- Cancer, Lipids, and Metabolism
- Glioma Diagnosis and Treatment
- Advanced Fluorescence Microscopy Techniques
- Cancer Genomics and Diagnostics
- Bioactive Compounds and Antitumor Agents
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- Advances in Cucurbitaceae Research
- Cancer-related gene regulation
- Sphingolipid Metabolism and Signaling
Memorial Sloan Kettering Cancer Center
2023-2024
University of Tennessee Health Science Center
2014-2023
Center for Cancer Research
2014-2019
Diabetic retinopathy is a microvascular disease that causes blindness. Using acid sphingomyelinase knockout mice, we reported ceramide generation critical for diabetic development. Here, in patients with proliferative retinopathy, identify vitreous imbalance pathologic long-chain C16-ceramides increasing and protective very C26-ceramides decreasing. generate pro-inflammatory/pro-apoptotic ceramide-rich platforms on endothelial surfaces. To geo-localize platforms, invented three-dimensional...
The management of pancreatic ductal adenocarcinoma (PDAC) is extremely poor due to lack an efficient therapy and development chemoresistance the current standard therapy, gemcitabine. Recent studies implicate intimate reciprocal interactions between epithelia underlying stroma paracrine Sonic hedgehog (SHH) signaling in producing desmoplasia PDAC. Herein, we report for first time that a nonsteroidal drug, ormeloxifene, has potent anticancer properties depletes tumor-associated stromal tissue...
Abstract Ormeloxifene is a clinically approved selective estrogen receptor modulator, which has also shown excellent anticancer activity, thus it can be an ideal repurposing pharmacophore. Herein, we report therapeutic effects of ormeloxifene on prostate cancer and elucidate novel molecular mechanism its activity. treatment inhibited epithelial-to-mesenchymal transition (EMT) process as evident by repression N-cadherin, Slug, Snail, vimentin, MMPs (MMP2 MMP3), β-catenin/TCF-4 transcriptional...
The pathogenesis of the marked pulmonary microvasculature injury, a distinguishing feature COVID-19 acute respiratory distress syndrome (COVID-ARDS), remains unclear. Implicated in pathophysiology diverse diseases characterized by endothelial damage, including ARDS and ischemic cardiovascular disease, ceramide particular palmitoyl (C16:0-ceramide) may be involved microvascular injury COVID-19. Using deidentified plasma lung samples from patients, profiling mass spectrometry was performed....
Prostate cancer (PrCa) metastasis is the major cause of mortality and morbidity among men. Metastatic PrCa cells are typically adopted for aberrant glucose metabolism. Thus, chemophores that reprogram altered metabolic machinery in can be useful agent repression metastasis. Herein, we report cucurbitacin D (Cuc D) effectively inhibits uptake lactate production metastatic via modulating This shift by Cuc was correlated with decreased expression GLUT1 its direct binding as suggested proficient...
Over 80% of colon cancer development and progression is a result the dysregulation β-catenin signaling pathway. Herein, for first time, we demonstrate that serine-threonine kinase, Protein Kinase D1 (PKD1), modulates functions to suppress growth. Analysis normal tissues reveals downregulation PKD1 expression in advanced stages its co-localization with crypts. This corresponds aberrant nuclear localization β-catenin. In-vitro investigation PKD1-β-catenin interaction cells reveal...
Light localization is a phenomenon which arises due to the interference effects of light waves inside disordered optical medium. Quantification degree in media widely used for characterizing structural disorder that media. Recently, this approach was extended analyze changes biological cell like heterogeneous media, with potential application cancer diagnostics. Confocal fluorescence microscopy construct “optical lattices,” represents 2‐dimensional refractive index map corresponding spatial...
Cancer progression and metastasis is profoundly influenced by protein kinase D1 (PKD1) metastasis-associated 1 (MTA1) in addition to other pathways. However, the nature of regulatory relationship between PKD1 MTA1, its resulting impact on cancer remains unknown. Here we present evidence establish that an upstream MTA1. Protein mRNA expression MTA1 PKD1-overexpressing cells were determined using western blotting reverse-transcription quantitative real-time PCR. Immunoprecipitation proximity...
The vascular endothelium from individual organs is functionally specialized, and it displays a unique set of accessible molecular targets. These serve as endothelial cell receptors to affinity ligands. To date, all identified have been proteins. Here, we show that an lung-homing peptide (CGSPGWVRC) interacts with C16-ceramide, bioactive sphingolipid mediates several biological functions. Upon binding surfaces, CGSPGWVRC triggers ceramide-rich platform formation, activates acid...
Abstract Objectives: Prostate cancer (PrCa) is the second most leading cause of cancer-related death in men United States. Chemotherapy (Docetaxel, Dox) currently common first-line therapeutic option. However, adverse side effects and chemo-resistance docetaxel limit its clinical use. Improving targeted delivery activity at tumor site using a nanoparticle system could be an attractive strategy for PrCa therapy. Specific Membrane Antigen (PSMA) highly overexpressed cells, thus molecular...
<p>Effect of ORM on proliferation various human PrCa cell lines as determined by counting the cells.</p>
Abstract Pancreatic cancer (PanCa) is one of the most fatal all cancers and ranked as fourth common cause related deaths among both men women in US. The management PanCa, exceptionally difficult due to extremely poor response available therapeutic modalities. Highly desmoplastic microenvironment pancreatic tumor causes suboptimal drug delivery increases chemo-resistance. Plumbagin (PL), a naturally occurring napthoquinone derived from root Plumbago zeylanica L., has showed potent preventive...
Abstract Pancreatic cancer (PanCa) is one of the deadliest cancers which accounts for 46,420 new cases and almost 39,590 deaths in United States 2014. There no effective treatment modality PanCa that can significantly improve overall survival rate patients. Paclitaxel a mitotic inhibitor widely used chemotherapy. Recent FDA approved regimen Abraxane® (paclitaxel nanoformulation) with gemcitabine has been increased patients from 6.7 months (Gemcitabine) to 8.5 (Abraxane® + Gemcitabine)....
Abstract Objective: Chemotherapy-induced emergence of drug resistance is one the major limitations to treat prostate cancer. Numerous studies report that autophagic cell death functions as a suppressor tumorigenesis in Although chemotherapeutic drugs inhibit growth cancer cells, they also activate chemokine, CCL2 which protects cells from death. Protein Kinase D1 (PKD1) plays multiple roles fundamental biological processes and known tumor Here, we for first time PKD1 inhibits expression...
Abstract Background: Breast cancer is the second most deadly in female, resulting over 39,000 deaths year 2012. Triple negative breast a subtype of characterized by its poor outcome and lack targeted therapies. New therapies are therefore an urgent unmet medical need for this patient population. Recent investigations into anti-cancer drug on repurposing pre-approved safe drugs treatment. We report first time that non-steroidal, selective estrogen receptor inhibitor, ormeloxifene (ORM)...
Intracellular structural alterations are hallmark of several disease conditions and treatment modalities. However, robust methods to quantify these changes scarce. In view this, we introduce a new method in biological cells through the widely used confocal microscopy. This novel employs optical eigenfunctions localization properties quantifies degree alterations, terms nano- micron scale intracellular mass density fluctuations, one single parameter. Such approach allows powerful way compare...
Abstract Background: Prostate cancer (PrCa) is the second leading cause of cancer-related deaths in American Men. Docetaxel (DTX) a standard first-line treatment for metastatic castration-resistant PrCa after failure hormone therapy. However, most patients who receive DTX experience only transient benefits and rapidly develop incurable drug resistance. Protein Kinase D1 (PKD1), one serine threonine kinases from PKD family highly expressed normal prostate tissues suppressed during...