A5013252958- Hormonal Regulation and Hypertension
- Adrenal and Paraganglionic Tumors
- Adrenal Hormones and Disorders
- Endometrial and Cervical Cancer Treatments
- Pituitary Gland Disorders and Treatments
- Peroxisome Proliferator-Activated Receptors
- Uterine Myomas and Treatments
- Endometriosis Research and Treatment
- Hypothalamic control of reproductive hormones
- Growth Hormone and Insulin-like Growth Factors
- Eicosanoids and Hypertension Pharmacology
- Reproductive Biology and Fertility
- Cervical Cancer and HPV Research
- Estrogen and related hormone effects
- Ovarian cancer diagnosis and treatment
- Retinoids in leukemia and cellular processes
- Blood Pressure and Hypertension Studies
- MicroRNA in disease regulation
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Adipose Tissue and Metabolism
- Renal and Vascular Pathologies
- Plant Reproductive Biology
- Pregnancy-related medical research
- Reproductive System and Pregnancy
- Nuclear Receptors and Signaling
Osaki Citizen Hospital
2020-2024
Tohoku University Hospital
2012-2021
Hokkaido University
2012-2021
Sapporo University
2021
Tohoku University
2010-2019
Hokkaido University Hospital
2010-2018
University of Mississippi Medical Center
2014-2017
G.V. (Sonny) Montgomery VA Medical Center
2014-2017
The University of Queensland
2016-2017
Southern Tohoku General Hospital
2015-2016
Relaxin is a hormone important for the growth and remodeling of reproductive other tissues during pregnancy. Although binding sites relaxin are widely distributed, nature its receptor has been elusive. Here, we demonstrate that two orphan heterotrimeric guanine nucleotide protein (G protein)–coupled receptors, LGR7 LGR8, capable mediating action through an adenosine 3′,5′-monophosphate (cAMP)–dependent pathway distinct from structurally related insulin insulin-like factor family ligand....
Glycoprotein hormone receptors, including LH receptor, FSH and TSH belong to the large G protein-coupled receptor (GPCR) superfamily but are unique in having a ectodomain important for ligand binding. In addition two recently isolated mammalian LGRs (leucine-rich repeat-containing, receptors), LGR4 LGR5, we further identified new paralogs, LGR6 LGR7, glycoprotein receptors. Phylogenetic analysis showed that there three LGR subgroups: known receptors; 6; third subgroup represented by LGR7....
Genomic DNA from 32 unrelated families with male-limited precocious puberty was examined for the previously described Asp-578-->Gly, Met-571-->Ile, and Thr-577-->Ile mutations in transmembrane helix 6 of human luteinizing hormone receptor (hLHR). Twenty-eight had inherited form disorder, these, 24 were found to have Asp-578-->Gly mutation. Four additional among remaining four sporadic cases disorder: an A-->C transversion resulting substitution leucine Ile-542 fifth helix,...
// Yosuke Konno 1,* , Peixin Dong 2,* Ying Xiong 3,* Fumihiko Suzuki 4,* Jiabin Lu 5 Muyan Cai Hidemichi Watari 1 Takashi Mitamura Masayoshi Hosaka Sharon J. B. Hanley 2 Masataka Kudo and Noriaki Sakuragi 1,2 Department of Gynecology, Hokkaido University, Sapporo, Japan Women's Health Educational System, 3 State Key Laboratory Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China 4 Obstetrics Tohoku Sendai, Pathology, * These authors contributed equally to...
Adrenal venous sampling is currently the only reliable method to distinguish unilateral from bilateral diseases in primary aldosteronism. In this study, we attempted determine whether peripheral plasma levels of 18-oxocortisol (18oxoF) and 18-hydroxycortisol could contribute clinical differentiation between aldosteronoma hyperaldosteronism 234 patients with aldosteronism, including computed tomography (CT)–detectable (n=113) (n=121), all whom underwent CT adrenal sampling. All aldosteronomas...
In ovarian cancer (OC) cells, Snail was reported to induce the epithelial-to-mesenchymal transition (EMT), which is a critical step in OC metastasis. At present little known about controlling expression cells by using specific microRNAs (miRNAs). We first used computational target prediction analysis identify 6 candidate miRNAs that bind 3′-untranslated region (3′-UTR) of mRNA. Among these miRNAs, two (miR-137 and miR-34a) with potential regulate were validated quantitative real-time PCR,...
In primary aldosteronism (PA), glomerular hyperfiltration due to excessive aldosterone is considered underestimate actual renal damage.Our objectives were determine the prevalence of chronic kidney disease (CKD) in PA and identify predictors decreasing estimated filtration rate (eGFR) after treatment.This was a 12-month prospective study patients with treated at Tohoku University Hospital.All according results adrenal venous sampling; 102 aldosterone-producing adenoma underwent...
Angiotensin (A) II plays a critical role in vascular remodeling, and its action is mediated by type 1 AII receptor (AT1R). Recently, 15-deoxy-(Delta)(12,14)-prostaglandin J(2) thiazolidinediones have been shown to be ligands for peroxisome proliferator-activated (PPAR)-gamma activate PPAR-gamma. In the present work, we studied effect of PPAR-gamma on AT1R expression rat smooth muscle cells (VSMCs). We observed that: 1) endogenous was significantly decreased both at messenger RNA protein...
Leydig cell hypoplasia (LCH) is a form of male pseudohermaphroditism in which differentiation and testosterone production are impaired. This report describes the first case nonsense mutation (A1635C) exon 11 human luteinizing hormone receptor (hLHR) gene two sisters with LCH. causes loss function by introducing stop codon at residue 545 transmembrane helix 5 hLHR. Surface expression truncated hLHR (hLHR-t545) embryonic kidney cells stably transfected cDNA encoding hLHR-t545 was diminished...
The objective of this study is to describe a technique for preserving the autonomic nerve systematically, including hypogastric nerves, pelvic splanchnic and plexus its vesical branches, based on anatomic considerations nerves innervating urinary bladder, in radical hysterectomies assess postsurgical bladder function. A nerve-sparing hysterectomy was carried out 27 consecutive patients with uterine cervical cancer treated between 2000 2002. FIGO stages disease consisted 10 stage Ib1, 6 Ib2,...
A natural retinoid all-trans retinoic acid (ATRA) regulates a variety of important cellular functions via receptor (RAR). ATRA has therapeutically been used against various malignancies including acute promyelocytic leukemia. Recently also recognized to be beneficial atherosclerotic vascular disorders. However, its effects on angiogenesis remain controversial. We therefore examined in vitro terms capillary-like tube formation using human umbilical vein endothelial cells (HUVECs)/normal...
Adrenal vein sampling (AVS) is fundamental for subtype diagnosis in patients with primary aldosteronism. AVS protocols vary between centers, especially diagnostic indices and use of adrenocorticotropic hormone (ACTH) stimulation. We investigated the role both continuous ACTH infusion bolus on performance interpretation a sample 76 confirmed In 36 aldosteronism patients, was performed under basal conditions after infusion, 40 IV bolus. Both determined an increase rate successful cannulation...
Adrenal venous sampling (AVS) is critical to determine the subtype of primary aldosteronism (PA). Central AVS (C-AVS)--that is, collection effluents from bilateral adrenal central veins (CV)--sometimes does not allow differentiation between aldosterone-producing adenomas (APA) and idiopathic hyperaldosteronism. To establish best treatment course, we have developed segmental (S-AVS); that collect tributaries CV intra-adrenal sources aldosterone overproduction. We then evaluated clinical...
EZH2 inhibition and reactivation of tumor suppressor microRNAs (miRNAs) represent attractive anti-cancer therapeutic strategies. We found that EZH2-suppressed let 7b miR-361, two likely suppressors, inhibited endometrial cancer (EC) cell proliferation invasion, abrogated stem cell-like properties. In EC cells, induced functioned together with YY1 to epigenetically suppress which upregulated Twist, a direct target miR-361. Treating cells GSK343, specific inhibitor, mimicked the effects...
Peripheral 18-oxocortisol (18oxoF) level could contribute to the detection of aldosterone-producing adenoma (APA) in patients with primary aldosteronism. However, peripheral 18oxoF varies among such patients, which is a big drawback concerning its clinical application. We studied 48 cases APA, 35 harboring KCNJ5 mutation, clarify significance and pathological parameters about 18oxoF. concentration ranged widely from 0.50 183.13 ng/dL correlated positively intratumoral areas stained for...
The human LH receptor (hLHR) is a member of the G protein-coupled receptors characterized by presence seven-transmembrane (TM) helices. Inactivating mutations hLHR lead to Leydig cell hypoplasia (LCH), form male pseudohermaphroditism resulting from failure fetal testicular differentiation. We have identified three in patient with LCH: deletion exon 8 (delta Exon 8), A872G transition Asn291Ser substitution extracellular domain, and C1847A transversion Ser616Tyr seventh TM helix. Nucleotide...
Glycoprotein hormone receptors are G protein-coupled with ligand-binding ectodomains consisting of leucine-rich repeats. The ectodomain is connected by a conserved cysteine-rich hinge region to the seven transmembrane (TM) region. Gain-of-function mutants luteinizing (LH) and thyroid-stimulating found in patients allowed identification residues important for receptor activation. Based on constitutively active mutations at Ser-281 receptor, we mutated serine LH (S277I) follicle-stimulating...
18-Oxocortisol (18-oxoF) is a derivative of cortisol (F) that produced by aldosterone synthase (CYP11B2). The potential for this steroid as biomarker differentiating patients with aldosterone-producing adenoma (APA) from those idiopathic hyperaldosteronism (IHA) has not been examined.We measured 18-oxoF, aldosterone, and F in plasma adrenal vein sampling (AVS) primary aldosteronism. We compared 18-oxoF levels 18-oxoF/F ratios their to differentiate APA IHA.This study F, AVS obtained...
Adrenal vein sampling (AVS) is the only reliable means to distinguish between aldosterone-producing adenoma and bilateral adrenal hyperplasia, two most common subtypes of primary aldosteronism (PA). AVS protocols are not standardized vary widely centers.The objective study was retrospectively investigate whether presence contralateral (CL) suppression aldosterone secretion associated with improved postoperative outcomes in patients who underwent unilateral adrenalectomy for PA.The carried...
To use segmental adrenal venous sampling (AVS) (S-AVS) of effluent tributaries (a version AVS that, in addition to helping identify aldosterone hypersecretion, also enables the evaluation intra-adrenal hormone distribution) detect and localize secretion.The institutional review board approved this study, all patients provided informed consent. S-AVS was performed 65 with primary aldosteronism (34 men; mean age, 50.9 years ± 11 [standard deviation]). A microcatheter inserted first-degree...
Overexpression of IQGAP1 and microRNA (miRNA) dysregulation are frequent in human tumors, but little is known about the role its relationship to miRNA endometrial carcinogenesis. We demonstrate that activates epithelial-mesenchymal transition (EMT) program miR-124 directly represses expression cancer (EC) cells. The overexpression stimulates EMT features enhances migration, invasion proliferation EC cells, whereas knocking down reverses inhibits these malignant properties. Using microarray...