A5103236846- Prenatal Screening and Diagnostics
- Genomic variations and chromosomal abnormalities
- MicroRNA in disease regulation
- Estrogen and related hormone effects
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Osteoarthritis Treatment and Mechanisms
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Engineering Applied Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Disease Resistance and Genetics
- Plant Pathogenic Bacteria Studies
- RNA Research and Splicing
- Genetic Syndromes and Imprinting
- Proteoglycans and glycosaminoglycans research
- Receptor Mechanisms and Signaling
- interferon and immune responses
- Plant-Microbe Interactions and Immunity
- Bone Metabolism and Diseases
- Genetic Mapping and Diversity in Plants and Animals
- Virus-based gene therapy research
- Cancer-related gene regulation
- Renin-Angiotensin System Studies
Tohoku University
1994-2022
DNA Chip Research (Japan)
2022
Fujita Health University Hospital
2020-2021
Fujita Health University
2018-2020
East Japan Railway (Japan)
2009-2015
Development Services Group (United States)
2015
National Agriculture and Food Research Organization
2014
In-Q-Tel
2011
National Institute for Agro-Environmental Sciences
1997-2000
Tokyo Institute of Technology
1996-1999
This study aimed to determine the expression profiles of microRNAs (miRNAs) in endometrial serous adenocarcinoma and examine association between miRNA clinical outcomes. Twenty‐one patients diagnosed with January 2001 December 2006 were enrolled. examined using microarray qRT‐PCR. levels correlated clinicopathological variables survival rates. A total 120 miRNAs differentially expressed compared normal endometria. Of these, 54 down‐regulated (>2‐fold), including miR‐101, miR‐10b*,...
There is an increased prevalence of imprinting disorders, such as Beckwith–Wiedemann syndrome, associated with human assisted reproductive technologies (ART). Work on animal models suggests that in vitro culture may be the source these errors. However, this study we report that, some cases, errors are inherited from father. We analyzed DNA methylation at seven autosomal imprinted loci and XIST locus 78 paired samples. In out seventeen cases where there was abnormal ART sample (41%),...
// Yosuke Konno 1,* , Peixin Dong 2,* Ying Xiong 3,* Fumihiko Suzuki 4,* Jiabin Lu 5 Muyan Cai Hidemichi Watari 1 Takashi Mitamura Masayoshi Hosaka Sharon J. B. Hanley 2 Masataka Kudo and Noriaki Sakuragi 1,2 Department of Gynecology, Hokkaido University, Sapporo, Japan Women's Health Educational System, 3 State Key Laboratory Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, P. R. China 4 Obstetrics Tohoku Sendai, Pathology, * These authors contributed equally to...
Basic fibroblast growth factor (bFGF) stimulates proliferation of chondrocytes and their extracellular matrix synthesis but inhibits terminal differentiation to hypertrophic cells (Kato, Y., Iwamoto, M., (1990) J. Biol. Chem. 265, 5903-5909). In the present study, we examined changes in bFGF binding during chondrocyte cytodifferentiation. cultures pelleted plate chondrocytes, 125I-bFGF 140-kDa receptors was observed mitotic matrix-forming stages decreased a very low level as became...
Evidence exists that sex steroids such as estrogens affect epithelial ovarian cancer. The expression profiles of the estrogen receptors (ER) and ERβ in particular have not been fully described. Therefore, our present study, we examined methylation status promoters 0K 0N, isoforms human carcinoma. We then correlated with ER status. Twelve carcinoma cell lines, six primary cultures surface cells (OSE), 64 cases tissues were examined. Bisulfite sequencing quantitative reverse...
Growth cartilage cells were isolated from the ribs of young rats and cultured at high cell density in Ham's F-12 medium supplemented with 10% fetal calf serum. During 7 days, glycosaminoglycans proteoglycans actively synthesized secreted, forming a metachromatic matrix. When together growth precultured biosynthetically prelabeled 35SO4(2-) their glycosaminoglycans, bone marrow caused release 35S-labeled material into culture medium. Glycosaminoglycan was also released by addition conditioned...
Endometrial serous adenocarcinoma (ESC) is aggressive and carries a poor prognosis. p53 frequently mutated in ESC. microRNAs (miRNAs) are direct target have been implicated cancer cell behavior. In this study, we compared miRNA expression levels ESC with the endometrial endometrioid (EEC) normal endometria. Six miRNAs were identified as having aberrant down-regulation specific to miR-34b being most pronounced. was found promoter hypermethylation, which when reversed, restored lines treated...
Rice blast is a destructive disease caused by Magnaporthe oryzae, and it has large impact on rice production worldwide. Compared with leaf resistance, our understanding of panicle resistance limited, only one gene, Pb1, isolated so far. The japonica cultivar Miyazakimochi shows to blast, yet the genetic components accounting for this remain be determined.In study, we evaluated populations derived from cross between Bikei 22 cultivar, which susceptible both blast. phenotypic analyses revealed...
Transcription factor E4TF1 is the human homolog of GABP and has been renamed hGABP (human GABP). composed two types subunits; hGABP-β1/E4TF1–53 ets-related protein hGABP-α/E4TF1–60. Both bind together to form an (α)2(β1)2 heterotetrameric complex on DNA activate transcription at specific promoters in vitro. Tetramer formation depends regions hGABPβ1; N-terminal region containing Notch/ankyrin-type repeats necessary for binding hGABPα C-terminal homodimerization. In this report, we...
Abstract Derepression of wild-type p53 by suppressing its negative inhibitor iASPP (Inhibitor apoptosis-stimulating protein p53) represents a potential therapeutic option for cervical cancer (CC). Here, we reported novel functional significance upregulation in tumorigenesis: acts as key promoter CC cell proliferation, epithelial-mesenchymal transition, invasion and stemness, interacting with to suppress p53-mediated transcription target genes reducing p53-responsive microRNA-34a levels....
The transcription factor human GA-binding protein (hGABP) is composed of two subunits, the Ets-related hGABPalpha, which binds to a specific DNA sequence, and either one hGABPalpha-associated hGABPbeta or hGABPgamma. DNA-binding hGABPalpha cannot affect by itself, but can modify hGABP-dependent in vitro vivo presence its associated subunits. In this study, co-transfection assays showed that ratio hGABPgamma affected from promoter containing hGABP binding sites. Biochemical analysis they bind...
Casein kinase II (CKII) is thought to regulate a broad range of transcription factors, but its mode action not well characterized. We previously showed that CKII co-purified with the ATF family factors using DNA-affinity latex beads. Here we report functional and physical interaction between factors. demonstrate binds through catalytic α α′ subunits basic leucine zipper (bZIP) DNA-binding domains many including ATF1. Kinetic analysis surface plasmon resonance sensor suggests loosely...
Specific polymerase chain reaction (PCR) primers targeting genomic DNA were selected for rapid, sensitive and specific detection of Burkholderia plantarii B. glumae, the causal agents bacterial seedling blight rice rot rice, respectively. The complete sequences spacer region between 16S 23S rRNA genes plantarii, gladioli, cepacia, caryophylli, andropogonis, solanacearum Pseudomonas corrugata, determined. Among strains from diverse geographical regions in Japan, degree sequence similarity was...
A microplate-ELISA was established for angiotensin (Ang) I using Ang -peroxidase conjugate, antiAng antibodies which could distinguish from II and III, 3,3′,5,5′-tetramethylbenzidine. be quantified in amounts as low 1 pg by the spectrophotometric method. The present assay system most sensitive rapid all systems described so far. applicable to determination of concentration human plasma abdominal fluid.
The effect of vanadate on proteoglycan synthesis by cultured rabbit costal chondrocytes was examined. Rabbit were seeded at low densities and grown to confluency in medium supplemented with 10% fetal bovine serum, then the serum concentration reduced 0.3%. At concentration, adopted a fibroblastic morphology. Addition 4 microM culture induced morphologic differentiation cells spherical chondrocytes, increased two- threefold incorporation [35S]sulfate [3H]glucosamine into large, chondroitin...
The Ets-related DNA-binding protein human GA-binding (hGABP) α interacts with the four ankyrin-type repeats of hGABPβ to form an hGABP tetrameric complex that stimulates transcription through adenovirus early 4 (E4) promoter. Using co-transfection assays, this study demonstrated mediated efficient activation from E4 promoter synergistically activating factor (ATF) 1 or cAMP response element-binding (CREB), but not ATF2/CRE-BP1. This synergy also partially occurred when hGABPα was used alone...
The retinoblastoma protein‐interacting zinc finger gene ( RIZ1 ) is considered a tumor suppressor gene. purpose of the present study was to examine expression and evaluate its clinicopathological significance in ovarian carcinoma. Immunohistochemistry quantitative reverse transcription–polymerase chain reaction (RT‐PCR) performed for examined. DNA methylation status also studied. All (6/6) normal, 5/9 benign, 4/9 borderline tissues were positive protein. In cancer 32.9% (54/164) . Decreased...
We analyzed the expression of steroid and xenobiotic receptor (SXR) in human uterine sarcomas evaluated its clinical significance. Forty-seven cases with archival specimens were examined for SXR using immunohistochemistry. All scored a semi-quantitative histological scoring (HSCORE) method. Specimens HSCORE >40 regarded as SXR-positive. Various clinicopathological variables, including status estrogen (ER)-α, progesterone (PR) Ki67 (MIB-1) examined. The mean HSCOREs carcinosarcoma (CS)...
Abstract Background Female carriers of a balanced X; autosome translocation generally undergo selective inactivation the normal X chromosome. This is because critical genes within autosomal region derivative chromosome would compromise cellular function. We here report female patient with bilateral retinoblastoma and severe intellectual disability who carries reciprocal X-autosomal translocation. Case presentation Cytogenetic molecular analyses, HUMARA (Human androgen receptor) assay,...
Objective Several studies have reported that retinoic acid (RA) might be used to treat malignancies. The effects of RA are mediated by the receptor (RAR), and RARα/RARβ especially acts as a tumor suppressor. However, little is known about its role in human endometrial cancer. Materials Methods In this study, we examined all-trans (ATRA) on progression cancer cell line, RL95-2 Hec1A. We then expression RARα RARβ 50 tissues using immunohistochemistry. Results found inhibitory ATRA...