A5058612697- Hemoglobinopathies and Related Disorders
- Erythrocyte Function and Pathophysiology
- Organometallic Complex Synthesis and Catalysis
- Metal complexes synthesis and properties
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Ovarian function and disorders
- RNA modifications and cancer
- Magnetism in coordination complexes
- Renin-Angiotensin System Studies
- Prenatal Screening and Diagnostics
- Commutative Algebra and Its Applications
- Inorganic and Organometallic Chemistry
- Epigenetics and DNA Methylation
- Asymmetric Hydrogenation and Catalysis
- Iron Metabolism and Disorders
- Mesenchymal stem cell research
- Blood groups and transfusion
- Algebraic structures and combinatorial models
- Receptor Mechanisms and Signaling
- Lanthanide and Transition Metal Complexes
- 3D Printing in Biomedical Research
- Cancer-related molecular mechanisms research
- Metal-Catalyzed Oxygenation Mechanisms
RIKEN BioResource Research Center
2015-2024
RIKEN
1993-2024
The Nakamura Hajime Eastern Institute
2009-2024
Kobayashi Pharmaceutical (Japan)
2016-2024
Hiroshima University
2022
University of Tsukuba
2000-2021
Genesis Research Institute
2020
Shinshu University
1972-2019
Meiji University
2001-2019
Nippon Soken (Japan)
2018
Transfusion of red blood cells (RBCs) is a standard and indispensable therapy in current clinical practice. In vitro production RBCs offers potential means to overcome shortage transfusable some situations also provide source free from possible infection or contamination by microorganisms. Thus, may become procedure the future. We previously reported successful establishment immortalized mouse erythroid progenitor cell lines that were able produce mature very efficiently. Here, we have...
Reactivating the fetal globin gene Mutation of adult-type genes causes sickle cell disease and thalassemia. Although treating these hemoglobinopathies with therapy is possible, there a pressing need for pharmacologic approaches to treat general patient populations. One promising approach reactivate repressed expression fetal-type hemoglobin (HbF) in adult erythroid cells. Masuda et al. reveal molecular mechanism governing HbF repression as mediated by LRF/ZBTB7A transcription factor. The...
Abstract With increasing worldwide demand for safe blood, there is much interest in generating red blood cells vitro as an alternative clinical product. However, available methods generation of from adult and cord progenitors do not yet provide a sustainable supply, current systems using pluripotent stem generate viable cells. We have taken approach, immortalizing early erythroblasts stable line, which provides continuous supply The immortalized differentiate efficiently into mature,...
MSCs and mesenchymal progenitor cells (MPCs) are studied for their potential in regenerative medicine. particular have great potential, because various reports shown that they can differentiate into many different cell types. However, the difference between stem/progenitor so-called fibroblasts is unclear. In this study, we found most of distinct populations primary fibroblast-like derived from human tissues, including lung, skin, umbilical cord, amniotic membrane, contained were able to at...
In chronic kidney disease (CKD), enhanced intrarenal angiotensin II (AngII) is involved in deterioration of renal function, but it difficult to measure it. For assessment the potential urinary angiotensinogen as a marker AngII activity, correlation plasma and renin-angiotensin system components, including angiotensinogen, with function was investigated 80 patients who had CKD were not treated blocking agents. Changes that induced by 14 d losartan treatment (25 mg/d) also measured 28...
We investigated the mechanism for non-proteolytic activation of human prorenin using five kinds antibodies. Each antigens, L1PPTDTTTFKRI11P, T7PFKRIFLKRMP17P, I11PFLKRMPSIRESLKER26P, M16PPSIRESLKER26P, and G27PVDMARLGPEWSQPM41P, was designed from tertiary structure predicted prorenin. These antibodies were labeled anti-01/06, anti-07/10, anti-11/26, anti-16/26, anti-27/41, respectively, their binding specificities. Inactive recombinant (0.1 nM) bound to various concentrations anti-27/41 at 4...
Theileria orientalis is one of the benign species that widely distributed in Japan and sometimes responsible for serious economic losses livestock industry. In present study, we surveyed current status T. infection grazing cattle eastern areas Hokkaido (Taiki, Otofuke, Shintoku, Shin-Hidaka districts) using molecular methods, as well traditional diagnosis. The genes encoding major piroplasm surface protein (MPSP) p23 were identified highly detectable polymerase chain reaction (PCR). Results...
Continuous human cell lines have been used extensively as models for biomedical research. In working with these lines, researchers are often unaware of the risk cross-contamination and other causes misidentification. To reduce this risk, there is a pressing need to authenticate comparing sample handled in laboratory previously tested sample. The American Type Culture Collection Standards Development Organization Workgroup ASN-0002 has developed Standard line authentication, recommending...
Objective Gout, caused by hyperuricaemia, is a multifactorial disease. Although genome-wide association studies (GWASs) of gout have been reported, they included self-reported cases in which clinical information was insufficient. Therefore, the relationship between genetic variation and subtypes remains unclear. Here, we first performed GWAS clinically defined only. Methods A conducted with 945 patients 1213 controls Japanese male population, followed replication study 1048 1334 controls....
Induced pluripotent stem (iPS) cells can be established from somatic cells. However, there is currently no strategy to generate corneal epithelial iPS In this study, we investigated whether could differentiated We tested 2 distinct sources: human adult dermal fibroblast (HDF)-derived (253G1) and limbal (HLEC)-derived (L1B41). first HLEC by introducing the Yamanaka 4 factors. Corneal were successfully induced stromal cell-derived inducing activity (SDIA) differentiation method, as Pax6+/K12+...
Although many of the frequently used pluripotency biomarkers are glycoconjugates, a glycoconjugate-based exploration novel cellular has proven difficult due to technical difficulties. This study reports unique approach for systematic overview all major classes oligosaccharides in glycome. The proposed method enabled mass spectrometry–based structurally intensive analyses, both qualitatively and quantitatively, N - O -linked glycans derived from glycoproteins, glycosaminoglycans,...
The amounts of progesterone, 5α-pregnane-3,20-dione, 3α-hydroxy-5α-pregnan-20-one, 20α-hydroxypregn-4-en-3-one, 20α-hydroxy-5α-pregnan-3-one and 5α-pregnane-3α,20α-diol in ovarian venous plasma at selected stages during the estrous cycle pregnancy rats, were determined by gas chromatography. Progesterone showed peak levels late proestrus early diestrus. former was associated with an increase 5α-pregnane-3,20-dione suggesting that due to LH stimulation. (2 µg) administered intravenously 30...