A5101544131- Glycosylation and Glycoproteins Research
- Sphingolipid Metabolism and Signaling
- Carbohydrate Chemistry and Synthesis
- Lysosomal Storage Disorders Research
- Lipid Membrane Structure and Behavior
- Enzyme Production and Characterization
- Erythrocyte Function and Pathophysiology
- Lipid metabolism and biosynthesis
- Algal biology and biofuel production
- Cellular transport and secretion
- Vestibular and auditory disorders
- Plant-Microbe Interactions and Immunity
- Retinoids in leukemia and cellular processes
- Peptidase Inhibition and Analysis
- Galectins and Cancer Biology
- Antioxidant Activity and Oxidative Stress
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Pneumonia and Respiratory Infections
- Microbial Metabolites in Food Biotechnology
- Biofuel production and bioconversion
- Plant Pathogenic Bacteria Studies
- Photochromic and Fluorescence Chemistry
- Peroxisome Proliferator-Activated Receptors
- Invertebrate Immune Response Mechanisms
Kyushu University
2015-2025
Teikyo University
2000-2024
Jichi Medical University
2023
Jichi Medical University Hospital
2023
Kariya Toyota General Hospital
2004-2021
Children's Medical Center
2020
Iwate University
2017-2019
Hokkaido University Hospital
2018
Kōchi University
2013-2017
Graduate School USA
2017
The vestibulo-ocular reflex (VOR) is an elementary reflex, which attracted the attention of a number classical neurophysiologists who studied, in particular, reflexive compensatory eye movements induced by head (cfMagnus 1924, Lorente de No 1931). major pathway for this well-defined trineuronal are, composed primary vestibu lar neurons, secondary vestibular and oculomotor neurons (Ra mon y Cajal 1909, N6 1931, 1933, Szentagothai 1943, 1950, 1964, Brodal & Pompeiano 1957). pioneering...
Several lines of epidemiological evidence have suggested that non‐alcoholic steatohepatitis (NASH) is closely associated with obesity in humans. However, the precise mechanisms progression NASH and its key metabolic abnormalities remain to be elucidated. We found long‐term high‐fat diet (HFD) exposure induces NASH, excess body weight, hyperinsulinemia hypercholesteremia mice. Longitudinal analysis model showed was induced after onset abnormalities. In addition, we expression MCP‐1 mRNA liver...
A novel glycosphingolipid-degrading enzyme was found in the cultured supernatant of Rhodococcus sp. G-74-2. It purified 34.7-fold from with 32.2% recovery by ammonium sulfate precipitation followed Sephadex G-100 chromatography. The demonstrated capable cleaving linkage between oligosaccharide and ceramide various acidic neutral glycosphingolipids, producing intact oligosaccharides ceramides. However, it noted to hardly make any attack on linkages monosaccharides ceramides (cerebrosides) or...
Although many of the frequently used pluripotency biomarkers are glycoconjugates, a glycoconjugate-based exploration novel cellular has proven difficult due to technical difficulties. This study reports unique approach for systematic overview all major classes oligosaccharides in glycome. The proposed method enabled mass spectrometry–based structurally intensive analyses, both qualitatively and quantitatively, N - O -linked glycans derived from glycoproteins, glycosaminoglycans,...
An overexpression system was recently developed to produce and purify recombinant, human acid ceramidase. In addition ceramide hydrolysis, the purified enzyme able catalyze synthesis using [14C]lauric sphingosine as substrates. Herein we report detailed characterization of this ceramidase-associated "reverse activity" provide evidence that reaction occurs in situ well vitro. The pH optimum reverse ∼5.5, compared with ∼4.5 for hydrolysis reaction. Non-ionic detergents zinc cations inhibited...
The main components of a new beamline for surface and interface crystal structure determination at SPring-8 are briefly described. Stages the monochromator modified making an incident X-ray intensity more stable experiments. Absolute photon flux densities were measured with energy. A ultrahigh vacuum system is introduced preliminary measurements from ordered oxygen on Pt (111) surface.
Abstract Two molecular species of endoglycoceramidase (designated as endoglycoceramidases I and II) were purified 32,700 43,000 times with overall recoveries 4.8 2.9%, respectively, from a culture fluid the mutant strain M-750 Rhodococcus sp., cultivated in absence inducers (ganglioside). After being stained Coomassie Brilliant Blue or silver-staining solution, each enzyme showed single protein band on polyacrylamide gel electrophoresis presence sodium dodecyl sulfate. The apparent weights,...
It is uncertain which β4-galactosyltransferase (β4GalT; gene name, B4galt), β4GalT-5 and/or β4GalT-6, responsible for the production of lactosylceramide (LacCer) synthase, functions in initial step ganglioside biosynthesis. Here, we generated conditional B4galt5 knockout (B4galt5 cKO) mice, using Nestin-Cre and crossed these with B4galt6 KO mice to generate 6 double (DKO) central nervous system (CNS). LacCer synthase activity major brain gangliosides were completely absent homogenates from...
ABSTRACT A marked decrease in the content of ceramide has been reported horny layer epidermis atopic dermatitis (AD). This impairs permeability barrier epidermis, resulting characteristic dry and easily antigen-permeable skin AD, since serves as major water-holding molecule extracellular space layer. On other hand, such patients is frequently colonized by bacteria, most typically Staphylococcus aureus , possessing genes those for sphingomyelinase, which are related to sphingolipid...
Previously, we reported two types of neutral ceramidase in mice, one solubilized by freeze-thawing and not. The former was purified as a 94-kDa protein from mouse liver, cloned (Tani, M., Okino, N., Mori, K., Tanigawa, T., Izu, H., Ito, M. (2000) <i>J. Biol. Chem.</i> 275, 11229–11234). In this paper, describe the purification, molecular cloning, subcellular distribution 112-kDa membrane-bound rat kidney, which completely insoluble freeze-thawing. open reading frame enzyme encoded...
The bioactive molecule sphingosine 1-phosphate (S1P) is abundantly stored in platelets and can be released extracellularly. However, although they have high (Sph) kinase activity, lack the de novo sphingolipid biosynthesis necessary to provide substrates. Here, we reveal a generation pathway for Sph, precursor of S1P, human platelets. Platelets incorporated extracellular 3H-labeled Sph much faster than megakaryoblastic cells rapidly converted it S1P. Furthermore, formed from plasma...
We report here a novel type of ceramidase of<i>Pseudomonas aeruginosa</i> AN17 isolated from the skin patient with atopic dermatitis. The enzyme was purified 83,400-fold an overall yield 21.1% culture supernatant strain AN17. After being stained silver staining solution, showed single protein band, and its molecular mass estimated to be 70 kDa on SDS-polyacrylamide gel electrophoresis. quite wide specificity for various ceramides,<i>i.e.</i> it hydrolyzed ceramides containing C12:0–C18:0...
We describe a novel enzyme that hydrolyzes the N-acyl linkage between fatty acids and sphingosine bases in ceramides of various sphingolipids. The was purified about 3000-fold with 5% recovery from culture filtrate newly isolated bacterium (Pseudomonas sp. TK4) by ammonium sulfate precipitation followed several steps high performance liquid chromatography. preparation completely free exoglycosidases, sphingomyelinase, proteases, showed single protein band corresponding to molecular mass 52...
We report here the molecular cloning, sequencing, and expression of gene encoding mouse neutral ceramidase, which has been proposed to function in sphingolipid signaling. A full-length cDNA ceramidase was cloned from a library liver using partial amino acid sequences purified ceramidase. The open reading frame 2,268 nucleotides encoded polypeptide 756 acids having nine putative N-glycosylation sites. Northern blot analysis revealed that mRNA expressed widely tissues, with especially strong...