A5075662890- Mesenchymal stem cell research
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Regulation of Appetite and Obesity
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Angiogenesis and VEGF in Cancer
- Neurotransmitter Receptor Influence on Behavior
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- Pharmacological Receptor Mechanisms and Effects
- HIV Research and Treatment
- Biochemical Analysis and Sensing Techniques
- Cancer, Hypoxia, and Metabolism
- Muscle Physiology and Disorders
- Pluripotent Stem Cells Research
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Amino Acid Enzymes and Metabolism
- Autophagy in Disease and Therapy
Zhujiang Hospital
2025
Southern Medical University
2025
Hunan Provincial Maternal and Child Health Hospital
2011-2024
Second Affiliated Hospital of Zhejiang University
2014-2024
Zhejiang University
2013-2024
Sun Yat-sen Memorial Hospital
2012-2024
Sun Yat-sen University
2012-2024
Central South University
2024
Shanxi Medical University
2024
Kiang Wu Hospital
2024
Recent studies have revealed that vascular cells can produce reactive oxygen species (ROS) through NAD(P)H oxidase, which may be involved in injury. However, the pathological role of oxidase diabetes or insulin-resistant state remains unknown. In this study, we examined effect high glucose level and free fatty acid (FFA) (palmitate) on ROS production cultured aortic smooth muscle (SMCs) endothelial (ECs) using electron spin resonance spectroscopy. Exposure SMCs ECs to a (400 mg/dl) for 72 h...
The expression pattern of mRNA encoding two orexin receptors (OX 1 R and OX 2 R) in the rat brain was examined. exhibited marked differential distribution. Within hypothalamus, is most abundant ventromedial hypothalamic nucleus whereas predominantly expressed paraventricular nucleus. High levels were also detected tenia tecta, hippocampal formation, dorsal raphe, locus coeruleus. mainly cerebral cortex, accumbens, subthalamic thalamic nuclei, anterior pretectal presence receptor hypothalamus...
Melanin-concentrating hormone (MCH) is a cyclic 19-aa hypothalamic neuropeptide derived from larger prohormone precursor of MCH (Pmch), which also encodes EI (NEI) and GE (NGE). Pmch-deficient (Pmch-/-) mice are lean, hypophagic, have an increased metabolic rate. Transgenic overexpressing Pmch hyperphagic develop mild obesity. Consequently, has been implicated in the regulation energy homeostasis. The 1 receptor (MCH1R) one two recently identified G protein-coupled receptors believed to be...
Agouti-related protein (AgRP), a neuropeptide abundantly expressed in the arcuate nucleus of hypothalamus, potently stimulates feeding and body weight gain rodents. AgRP is believed to exert its effects through blockade signaling by α-melanocyte-stimulating hormone at central nervous system (CNS) melanocortin-3 receptor (Mc3r) Mc4r. We generated AgRP-deficient (Agrp−/−) mice examine physiological role AgRP. Agrp−/− are viable exhibit normal locomotor activity, growth rates, composition, food...
Abstract Our group recently reported positive therapeutic benefit of human endometrium-derived mesenchymal stem cells (EnMSCs) delivered to infarcted rat myocardium, an effect that correlated with enhanced secretion protective cytokines and growth factors compared parallel cultures bone marrow MSCs (BMMSCs). To define more precisely the molecular mechanisms EnMSC therapy, in present study, we assessed paracrine properties derived from endometrium, marrow, adipose tissues a model myocardial...
Rationale : Autophagy can preserve cell viability under conditions of mild ischemic stress by degrading damaged organelles for ATP production, but severe ischemia, it promote death and worsen cardiac performance. Mesenchymal stem cells (MSCs) are cardioprotective when tested in animal models myocardial infarction, whether these benefits occur through the regulation autophagy is unknown. Objective: To determine transplanted MSCs reduce rate autophagic degradation (autophagic flux) infarcted...
Hypoxia treatment enhances paracrine effect of mesenchymal stem cells (MSCs). The aim this study was to investigate whether exosomes from hypoxia-treated MSCs (ExoH) are superior those normoxia-treated (ExoN) for myocardial repair. Mouse bone marrow-derived were cultured under hypoxia or normoxia 24 h, and conditioned media intramyocardially injected into infarcted heart C57BL/6 mouse. ExoH resulted in significantly higher survival, smaller scar size better cardiac functions recovery....
The effectiveness of transplanted bone marrow mesenchymal stem cells (MSCs) for cardiac repair has been limited; thus, strategies optimizing stem-cell-based myocardial therapy are needed.The present study was designed to test our central hypothesis that hypoxia-preconditioned MSCs (HP-MSCs) more effective than cultured under ambient oxygen levels the treatment injury in a large-scale (N=49), long-term (9 months), nonhuman primate (Cynomolgous monkeys) investigation.MSCs were engineered...
Bone marrow mesenchymal stem cell (MSC)-derived small extracellular vesicles (sEVs) have been widely used for treating myocardial infarction (MI). However, low retention and short-lived therapeutic effects are still significant challenges. This study aimed to determine whether incorporation of MSC-derived sEVs in alginate hydrogel increases their the heart thereby improving effects. Methods: The optimal sodium incorporating system was determined by its release ability rheology hydrogel. Ex...
Extracellular vesicles containing miR-486-5p augment angiogenesis after MI in mice and nonhuman primates by targeting fibroblastic Mmp19 .
By using a combination of genetic, pharmacological, and anatomical approaches, we show that the melanocortin 4 receptor (MC4R), implicated in control food intake energy expenditure, also modulates erectile function sexual behavior. Evidence supporting this notion is based on several findings: (i) highly selective non-peptide MC4R agonist augments activity initiated by electrical stimulation cavernous nerve wild-type but not Mc4r-null mice; (ii) copulatory behavior enhanced administration...
Insulin resistance and hypertension are considered as prototypical "diseases of civilization" that manifested in the modern environment plentiful food sedentary life. The human propensity for insulin is a product, at least part, our evolutionary history. Adaptation to ancient lifestyle characterized by low sodium, low-calorie supply physical stress injury response has driven evolution shape preserve thrifty genotype, which favorite with energy-saving sodium conservation. As civilization...
Two non-peptide substance P antagonists exhibit opposite rank orders of potency for the human and rat neurokinin-1 receptors. CP-96,345 shows selectivity receptor, whereas RP67580 receptor. Amino acid sequence comparison two receptors reveals 22 divergent residues. To elucidate molecular basis species these antagonists, residues in receptor were substituted by homologs. Analysis mutant revealed that substitution 2 (V116L I290S) transmembrane domain is both necessary sufficient to reproduce...
Abstract Microbial utilization of renewable one-carbon compounds, such as methane, methanol, formic acid, and CO 2 , has emerged a potential approach to increase the range carbon sources for bioproduction address climate change issues. Here, we modify natural serine cycle present in methylotrophs build an adapted pathway Escherichia coli which allows microorganism condense methanol (or formate) together with bicarbonate produce various products. We introduce modified into E. demonstrate its...
Human pluripotent stem cell-derived cardiovascular progenitor cells (hPSC-CVPCs) should be thoroughly investigated in large animal studies before testing clinical trials.The main of this study is to clarify whether hPSC-CVPCs can engraft for long time the heart primates after myocardial infarction (MI) and compare effectiveness safety immunosuppression with cyclosporine alone or multiple-drug regimen (MDR) containing cyclosporine, methylprednisolone, basiliximab cynomolgus monkeys that had...
Mesenchymal stem cells (MSCs) senescence is an age-related process that impairs the capacity for tissue repair and compromises clinical use of autologous MSCs regeneration. Here, we describe effects SIRT1, a NAD(+)-dependent deacetylase, on senescence. Knockdown SIRT1 in young induced cellular inhibited cell proliferation whereas overexpression aged reversed phenotype stimulated proliferation. These results suggest plays key role modulating age-induced Aging-related proteins, P16 P21 may be...