A5100762934- Urological Disorders and Treatments
- Pediatric Urology and Nephrology Studies
- Kidney Stones and Urolithiasis Treatments
- Tissue Engineering and Regenerative Medicine
- Urologic and reproductive health conditions
- Porphyrin Metabolism and Disorders
- Biomedical Research and Pathophysiology
- Electrospun Nanofibers in Biomedical Applications
- Ureteral procedures and complications
- CRISPR and Genetic Engineering
- Urinary and Genital Oncology Studies
- Urinary Tract Infections Management
- Medical Imaging Techniques and Applications
- Advanced X-ray and CT Imaging
- Sexual Differentiation and Disorders
- Advanced Radiotherapy Techniques
- Testicular diseases and treatments
- Radiomics and Machine Learning in Medical Imaging
- Virus-based gene therapy research
- Renal and Vascular Pathologies
- RNA regulation and disease
- RNA Interference and Gene Delivery
- Metabolism and Genetic Disorders
- Genital Health and Disease
- Organ Donation and Transplantation
Children's Hospital of Fudan University
2023-2025
Hebei Normal University
2025
XinHua Hospital
2015-2024
Shanghai Jiao Tong University
2014-2023
National Health and Family Planning Commission
2019-2022
Qingdao University
2022
University of Pennsylvania
2017-2020
Hong Kong Sanatorium and Hospital
2012-2020
Peking University First Hospital
2019
Peking University
2019
Summary Primary biliary cirrhosis (PBC) is an organ-specific autoimmune liver disease characterized by progressive loss of intrahepatic small bile ducts. Cellular immune mechanisms involving T cell reaction are thought to be involved significantly in the pathogenesis PBC. Recent studies have independently revealed enhanced helper type 17 (Th17) response and weakened regulatory (Treg) some diseases, indicating a role Th17/Treg imbalance autoimmunity. This prompted us investigate whether...
Genome-wide association studies (GWAS) have identified loci associated with kidney diseases, but the causal variants, genes, and pathways involved remain elusive. Here, we a disease gene called pancreatic progenitor cell differentiation proliferation factor (PPDPF) through integrating GWAS on function multiomic analysis. PPDPF was predominantly expressed in healthy proximal tubules of human mouse kidneys via single-cell Further investigations revealed that functioned as thiol-disulfide...
We fabricated a novel vascular endothelial growth factor (VEGF)-loaded poly(lactic-co-glycolic acid) (PLGA)-nanoparticles (NPs)-embedded thermo-sensitive hydrogel in porcine bladder acellular matrix allograft (BAMA) system, which is designed for achieving sustained release of VEGF protein, and embedding the protein carrier into BAMA. identified optimized various formulations process parameters to get preferred particle size, entrapment, polydispersibility VEGF-NPs, incorporated VEGF-NPs...
Hereditary tyrosinemia type I (HTI) is a metabolic genetic disorder caused by mutation of fumarylacetoacetate hydrolase (FAH). Because the accumulation toxic metabolites, HTI causes severe liver cirrhosis, failure, and even hepatocellular carcinoma. an ideal model for gene therapy, several strategies have been shown to ameliorate symptoms in animal models. Although CRISPR/Cas9-mediated genome editing able correct Fah mouse models, WT Cas9 induces numerous undesired mutations that raised...
Abstract Graft contracture is a common problem associated with the regeneration processes of tissue-engineered bladders. Currently, most strategies used for incorporating bioactive molecules into biomaterial designs do not work during all phases tissue regeneration. In this study, we growth factor-PLGA nanoparticle thermo-sensitive gel system (i.e., BAM incorporated VEGF and bFGF-loaded PLGA nanoparticles mixed hydrophilic gel) to promote bladder in rabbit model. At 4 12 weeks after surgery,...
Docetaxel (DTX) is a very important member of taxoid family. Despite several alternative delivery systems reported recently, DTX formulated by Polysorbate 80 and alcohol (Taxotere®) still the most frequent administration in clinical practice. In this study, we incorporated into 80/Phospholipid mixed micelles compared its structural characteristics, pharmacokinetics, biodistribution, blood compatibility with conventional counterparts. Results showed that loaded possessed mean size...
Curcumin, a compound extracted from the roots of Zingiberaceae, has been proposed as treatment for tissue injury but studies are yet to be done on its effect tendon healing. Therefore, we performed series experiments test our hypothesis that curcumin positive effects repair. Patellar window defect was created in Sprague–Dawley rats and these were divided into two groups: (i) control (ii) curcumin-treated. Curcumin (100 mg/kg body weight) applied by oral gavage. Its potential promoting...
Objective: To evaluate the clinical features and prognosis of congenital anterior urethrocutaneous fistulas (CAUF) patients. Methods: This is a retrospective chart review patients diagnosed with CAUF underwent surgical repair by author between October 2017 December 2021 that was performed at single tertiary-care institution. The medical records were reviewed to their characteristics outcomes. Results: Eleven included. median age 41 months (11-84 months) months. Subcoronal observed in 1...
Primary hyperoxaluria type 1 (PH1) is a rare inherited liver disorder caused by alanine glyoxylate aminotransferase (AGT) dysfunction, leading to accumulation of which then converted into oxalate. Excessive oxalate results in kidney damage due deposition crystals. We have developed an mRNA-based protein replacement therapy for PH1 restore normal glycine metabolism. Sequence optimized human AGT mRNA ( hAGT mRNA) was encapsulated lipopolyplex (LPP) and produced functional enzyme peroxisomes....
Base editing technology efficiently generates nucleotide conversions without inducing excessive double-strand breaks (DSBs), which makes it a promising approach for genetic disease therapy. In this study, we generated novel hereditary tyrosinemia type 1 (HT1) mouse model, contains start codon mutation in the fumarylacetoacetate hydrolase (Fah) gene by using an adenine base editor (ABE7.10). To investigate feasibility of recombinant adeno-associated virus (rAAV)-mediated therapy, intein-split...
Abstract Background Primary hyperoxaluria type 1 (PH1) is a rare genetic disorder caused by endogenous overproduction of hepatic oxalate, leading to hyperoxaluria, recurrent calcium oxalate kidney stones, and end‐stage renal disease. Lactate dehydrogenase (LDH) an ideal target for diminishing production as it responsible glyoxylate conversion in the liver, last step metabolism. Here, we investigated therapeutic efficacy potential side effects clustered regularly interspaced short palindromic...
Targeting key enzymes that generate oxalate precursors or substrates is an alternative strategy to eliminate primary hyperoxaluria type I (PH1), the most common and life-threatening of hyperoxaluria. The compact Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) from <i>Prevotella</i> <i>Francisella 1</i> (Cpf1) protein simplifies multiplex gene editing allows for all-in-one adeno-associated virus (AAV) delivery. We hypothesized capabilities Cpf1 system could help minimize...
Purpose This study assessed the use of vascular endothelial growth factor (VEGF) gene-modified progenitor cells (EPCs) seeded onto bladder acellular matrix grafts (BAMGs), to enhance blood supply in tissue-engineered bladders a porcine model. Methods Autologous peripheral EPCs were isolated, cultured, expanded, characterized, and modified with VEGF gene using an adenovirus vector. The expression was examined reverse transcriptase polymerase chain reaction (RT-PCR) enzyme-linked immunosorbent...