A5034354210- CRISPR and Genetic Engineering
- Urban Heat Island Mitigation
- Urban Green Space and Health
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Noise Effects and Management
- Virus-based gene therapy research
- Building Energy and Comfort Optimization
- Remote Sensing and Land Use
- Land Use and Ecosystem Services
- RNA regulation and disease
- Plant Virus Research Studies
- Wind and Air Flow Studies
- Influenza Virus Research Studies
- Nanofabrication and Lithography Techniques
- Biochemical and Molecular Research
- Viral Infections and Immunology Research
- Insect Resistance and Genetics
- interferon and immune responses
- Climate Change and Health Impacts
- Viral Infectious Diseases and Gene Expression in Insects
- Global Trade and Competitiveness
- Metabolism and Genetic Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Meteorological Phenomena and Simulations
Nanjing University of Information Science and Technology
2025
East China Normal University
2015-2024
Shanghai Electric (China)
2024
Jewish General Hospital
2023
Jiangsu University
2022
Key Laboratory of Guangdong Province
2016
South China Agricultural University
2016
University of Science and Technology of China
2013
Shanghai Medical College of Fudan University
2013
Shanghai Public Health Clinical Center
2013
Significance A unique avian-origin H7N9 influenza virus caused 134 human infections with 44 deaths. The host factors contributing to moderate vs. severe disease are not clear. Here, we show that severity was associated a higher level of cytokines/chemokines. We demonstrate the cytokines in infected lung were 100- 1,000-fold than those plasma. Furthermore, found IFN-induced transmembrane protein-3 (IFITM3) C/C genotype clinical outcome, as reflected by reduced time seeking medical aid; more...
Abstract Hybrid rice breeding offers an important strategy to improve production, in which the cultivation of a male sterile line is key success cross-breeding. CRISPR/Cas9 systems have been widely used target-site genome editing, whereas their application for crop genetic improvement has rarely reported. Here, using system, we induced specific mutations TMS5 , most applied thermo-sensitive genic sterility (TGMS) gene China, and developed new “transgene clean” TGMS lines. We designed 10...
Solar energy, as a clean and renewable resource is becoming increasingly important in the global context of climate change energy crisis. Utilization solar urban areas great importance planning, environmental conservation, sustainable development. However, available spaces for panel installation cities are quite limited except building roofs. Furthermore, complex 3D morphology greatly affects sunlit patterns on roofs, especially downtown areas, which makes determination roof potential...
Abstract Base editors, including dual base are innovative techniques for efficient conversions in genomic DNA. However, the low efficiency of A-to-G conversion at positions proximal to protospacer adjacent motif (PAM) and A/C simultaneous editor hinder their broad applications. In this study, through fusion ABE8e with Rad51 DNA-binding domain, we generate a hyperactive ABE (hyABE) which offers improved editing region (A 10 -A 15 ) PAM, 1.2- 7-fold improvement compared ABE8e. Similarly,...
With the development of emerging technologies such as artificial intelligence, traditional medical service model is gradually evolving into a "digital intelligence" paradigm, giving rise to innovations represented by Internet hospitals. However, current physician allocation methods often rely on fixed scheduling systems and simple assignment schemes, which significantly limit flexibility efficiency intelligence care". To address this issue, study leverages predict-then-optimize framework...
The continuous expansion of impervious artificial surfaces in cities has significantly influenced the urban thermal environment. This paper examines spatiotemporal variation diurnal surface heat island (SUHI) Shanghai from 1989 to 2013, a period during which city experienced drastic development changes. A remote sensing approach was taken derive spatial patterns Shanghai’s land temperature (LST) Landsat Thematic Mapper (TM) images and Operational Land Imager (OLI) data. LST pattern further...
Mitochondrial base editing is a powerful technology, but the current A-to-G mitochondrial DNA (mtDNA) editors are reluctant to achieve high efficiency, which critical for mtDNA engineering. Through laboratory evolution, TadA-8e variants were discovered with substantially increased activity and expanded targeting compatibility, especially previously unfavorite sequence contexts, when applied both nuclear ABEs. Further engineering of (eTd-mtABEs) dramatically reduced RNA off-targeting effects...
Abstract Adenine base editors (ABEs) catalyze A-to-G transitions showing broad applications, but their bystander mutations and off-target editing effects raise the concerns of safety issues. Through structure-guided engineering, we found ABE8e with an N108Q mutation reduced both adenine cytosine editing, introduction additional L145T (ABE9), further refined window to 1-2nt eliminated editing. Importantly, ABE9 induced very minimal RNA undetectable Cas9-independent DNA effects, which mainly...