A5014756786- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- Animal Virus Infections Studies
- Respiratory viral infections research
- vaccines and immunoinformatics approaches
- Immune Response and Inflammation
- Animal Disease Management and Epidemiology
- Vaccine Coverage and Hesitancy
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Wireless Communication Security Techniques
- Advanced MIMO Systems Optimization
- Long-Term Effects of COVID-19
- Virus-based gene therapy research
- Wireless Signal Modulation Classification
- interferon and immune responses
- Advanced Wireless Communication Technologies
- Cooperative Communication and Network Coding
- Immune responses and vaccinations
- SARS-CoV-2 detection and testing
- COVID-19 epidemiological studies
Guangzhou Medical University
2020-2025
State Key Laboratory of Respiratory Disease
2020-2025
First Affiliated Hospital of Guangzhou Medical University
2020-2025
Tsinghua University
2024-2025
Peter Doherty Institute
2015-2024
The University of Melbourne
2014-2024
Guangzhou Experimental Station
2022-2024
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2022-2024
Guangzhou First People's Hospital
2024
Wannan Medical College
2021-2023
Significance A unique avian-origin H7N9 influenza virus caused 134 human infections with 44 deaths. The host factors contributing to moderate vs. severe disease are not clear. Here, we show that severity was associated a higher level of cytokines/chemokines. We demonstrate the cytokines in infected lung were 100- 1,000-fold than those plasma. Furthermore, found IFN-induced transmembrane protein-3 (IFITM3) C/C genotype clinical outcome, as reflected by reduced time seeking medical aid; more...
Abstract The avian origin A/H7N9 influenza virus causes high admission rates (>99%) and mortality (>30%), with ultimately favourable outcomes ranging from rapid recovery to prolonged hospitalization. Using a multicolour assay for monitoring adaptive innate immunity, here we dissect the kinetic emergence of different effector mechanisms across spectrum H7N9 disease recovery. We find that diversity response contribute resolution survival. Patients discharged within 2–3 weeks have early...
Mucosal-associated invariant T (MAIT) cells are innate-like lymphocytes known to elicit potent immunity a broad range of bacteria, mainly via the rapid production inflammatory cytokines. Whether MAIT contribute antiviral is less clear. Here we asked whether produce cytokines/chemokines during severe human influenza virus infection. Our analysis in patients hospitalized with avian H7N9 pneumonia showed that individuals who recovered had higher numbers CD161(+)Vα7.2(+) peripheral blood...
Mucosal associated invariant T (MAIT) cells are evolutionarily-conserved, innate-like lymphocytes which abundant in human lungs and can contribute to protection against pulmonary bacterial infection. MAIT also activated during viral infections, yet it remains unknown whether play a significant protective or even detrimental role infections vivo. Using murine experimental challenge with two strains of influenza A virus, we show that accumulate early infection, upregulation CD25, CD69 Granzyme...
Abstract T-cell immunity is important for recovery from COVID-19 and provides heightened re-infection. However, little known about the SARS-CoV-2-specific in virus-exposed individuals. Here we report virus-specific CD4 + CD8 memory recovered patients close contacts. We also demonstrate size quality of pool are larger better than those proliferation capacity, responses contacts readily distinguishable healthy donors, suggesting able to gain against SARS-CoV-2 despite lacking a detectable...
Abstract Severe influenza A virus (IAV) infection is associated with immune dysfunction. Here, we show circulating CD8 + T-cell profiles from patients hospitalized avian H7N9, seasonal IAV, and vaccinees. Patient survival reflects an early, transient prevalence of highly activated CD38 HLA-DR PD-1 T cells, whereas the prolonged persistence this set found in ultimately fatal cases. Single-cell cell receptor (TCR)-αβ analyses cells similar TCRαβ diversity but differential clonal expansion...
Background: Coronavirus disease 2019 (COVID-19) has been a global pandemic disease, with more than 4 million cases and nearly 300,000 deaths. Little is known about COVID-19 in patients chronic obstructive pulmonary (COPD). We aimed to evaluate the influence of preexisting COPD on progress outcomes COVID-19. Methods: This was multicenter, retrospective, observational study. enrolled 1,048 aged 40 years above, including 50 998 without COPD, confirmed via high-throughput sequencing or real-time...
CD8+ T cells recognizing antigenic peptides derived from conserved internal viral proteins confer broad protection against distinct influenza viruses. As memory change throughout the human lifetime and across tissue compartments, we investigated how cell receptor (TCR) composition diversity relate to anatomical sites immunological phases of life. We used ex vivo peptide-HLA tetramer magnetic enrichment, single-cell multiplex RT-PCR for both TCR-alpha (TCRα) TCR-beta (TCRβ) chains, new...
Background: Public health measures (such as wearing masks, physical distancing, and isolation) have significantly reduced the spread of coronavirus disease-2019 (COVID-19), but impact public on other respiratory infectious diseases is unclear. Objective: To assess correlation between incidence in China during COVID-19 pandemic. Methods: We collected data from National Health Construction Commission number patients with six (measles, tuberculosis, pertussis, scarlet fever, influenza, mumps)...
Glycolipid-peptide conjugate vaccines protect against rodent malaria by generating large numbers of liver CD8 + T RM cells.
Abstract How innate and adaptive immune responses work in concert to resolve influenza disease is yet be fully investigated one single study. Here, we utilize longitudinal samples from patients hospitalized with acute understand these responses. We report the dynamics of 18 important parameters, related clinical, genetic virological factors, across different severity levels. Influenza correlates increases IL-6/IL-8/MIP-1α/β cytokines lower antibody Robust activation circulating T follicular...
Waned vaccine-induced immunity and emerging severe acute respiratory syndrome coronavirus 2 variants with potential for immune escape pose a major threat to the disease (COVID-19) pandemic. Here, we showed that humoral components, including anti-S + N, anti-RBD IgG, neutralizing antibodies (NAbs), gradually waned decreased capacity against Omicron at 3 6 months after two inactivated COVID-19 vaccinations. We evaluated boosting strategies either third dose of vaccine (homologous, I-I-I) or...
Abstract The immunoprotective components control COVID-19 disease severity, as well long-term adaptive immunity maintenance and subsequent reinfection risk discrepancies across initial remain unclarified. Here, we longitudinally analyzed SARS-CoV-2-specific immune effectors during the acute infection convalescent phases of 165 patients with categorized by severity. We found that early robust CD4 + CD8 T cell responses ameliorate progression shortened hospital stay, while delayed attenuated...
Abstract The EG.5.1 variant of severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2) has been prevalent since mid‐July 2023 in the United States and China. BA.2.86 become a major concern because it is 34 mutations away from parental BA.2 >30 XBB.1.5. There an urgent need to evaluate whether immunity population current vaccines are protective against BA.2.86. Based on cohort two breakthrough‐infected groups, levels neutralizing antibodies (NAbs) different subvariants were measured...
Respiratory infections cause significant morbidity and mortality, yet it is unclear why some individuals succumb to severe disease. In patients hospitalized with avian A(H7N9) influenza, we investigated early drivers underpinning fatal Transcriptomics strongly linked oleoyl-acyl-carrier-protein (ACP) hydrolase (OLAH), an enzyme mediating fatty acid production, after hospital admission, persisting until death. Recovered had low OLAH expression throughout hospitalization. High levels were also...
ABSTRACT A reassortant avian influenza virus (designated FPV NS GD), carrying the NS-segment of highly pathogenic (HPAIV) strain A/Goose/Guangdong/1/96 (GD; H5N1) in genetic background HPAIV A/FPV/Rostock/34 (FPV; H7N1), was rescued by reverse genetics. Remarkably, contrast to recombinant wild-type (rFPV), able replicate more efficiently different human cell lines and primary mouse epithelia cells without prior adaptation. Moreover, GD caused disease death experimentally infected mice...
Significance The upper respiratory tract (URT) is the first contact site for inhaled pathogens and intranasal vaccines, serviced by a network of lymphoid-tissues, including draining lymph nodes nasal-associated lymphoid tissues (NALTs). Whether these structures have distinct roles in facilitating T-cell immunity to antigens unclear. We show, following antigen delivery into URT, NALTs failed support naïve priming; however, they supported recall expansion memory T cells. Although URT may not...
There are several limitations in the data from this case series.First, as is common with series, selection bias possible.Second, there was no control intervention, and study sample small.Third, it uncertain whether these patients would have improved without prone positioning, although rapid change, within 1 hour, after proning suggestive of a favorable impact.Fourth, measures patient dyspnea or comfort positioning were not collected.Fifth, to minimize documentation burden on nursing-staff...
The rapid widespread Omicron subvariant BA.5 of SARS-CoV-2 has become a potential imminent pandemic threat, but available vaccines lack high efficacy against this subvariant. Thus, it is urgent to find highly protective vaccination strategies within vaccines. Here, by using pseudovirus neutralization assay, we demonstrated that the aerosol inhalation adenoviral vector COVID-19 vaccine after two dose inactivated (I-I-Ad5) led higher levels neutralizing antibodies D614G strain (2041.00[95% CI,...
Abstract Understanding the decay and maintenance of long-term SARS-CoV-2 neutralizing antibodies in infected or vaccinated people how vaccines protect against other variants is critical for assessing public vaccination plans. Here, we measured different plasm antibody levels 2 12 months after disease onset, including anti-RBD, anti-N, total antibodies, two neutralizing-antibody clusters. We found that declined more slowly than anti-RBD anti-N IgG, clusters decayed even antibodies....
Indigenous populations, including Australians, are highly susceptible to severe influenza disease and the underlying mechanisms unknown. We studied immune genetic factors that could predicate in Australians enrolled LIFT study: looking into T-cell immunity. To examine CD8(+) immunity, we characterised human leukocyte antigen (HLA) profiles. HLA typing confirmed previous studies showing predominant usage of HLA-A*02:01, 11:01, 24:02, 34:01 HLA-B*13:01, 15:21, 40:01/02, 56:01/02 Australians....