A5086047963- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Malaria Research and Control
- Cancer Immunotherapy and Biomarkers
- vaccines and immunoinformatics approaches
- Autophagy in Disease and Therapy
- Pancreatic function and diabetes
- Biomedical Research and Pathophysiology
- Cytomegalovirus and herpesvirus research
- CAR-T cell therapy research
- Mycobacterium research and diagnosis
- Invertebrate Immune Response Mechanisms
- Immune cells in cancer
- Vector-borne infectious diseases
- Complement system in diseases
- Genetic and Kidney Cyst Diseases
- Cancer-related molecular mechanisms research
- Cell Adhesion Molecules Research
- Parasites and Host Interactions
- Hepatitis B Virus Studies
- Advanced Glycation End Products research
- IL-33, ST2, and ILC Pathways
Olivia Newton-John Cancer Wellness & Research Centre
2021-2024
La Trobe University
2021-2023
The University of Melbourne
2020-2021
Centre d’Immunologie de Marseille-Luminy
2014-2021
Australian Research Council
2020-2021
Peter Doherty Institute
2020-2021
ARC Centre of Excellence in Advanced Molecular Imaging
2020-2021
Inserm
2014-2020
Centre National de la Recherche Scientifique
2020
Aix-Marseille Université
2014-2015
Naive CD8+ T cell priming during tumor development or many primary infections requires cross-presentation by XCR1+ dendritic cells (DCs). Memory lymphocytes (mCTLs) harbor a lower activation threshold as compared with naive cells. However, whether their recall responses depend on DCs is unknown. By using new mouse model allowing fluorescent tracking and conditional depletion of DCs, we demonstrate differential requirement these for mCTL secondary different pathogens. were instrumental to...
Glycolipid-peptide conjugate vaccines protect against rodent malaria by generating large numbers of liver CD8 + T RM cells.
Autophagy-related genes have been closely associated with intestinal homeostasis. BECLIN1 is a component of Class III phosphatidylinositol 3-kinase complexes that orchestrate autophagy initiation and endocytic trafficking. Here we show epithelium-specific deletion in adult mice leads to rapid fatal enteritis compromised gut barrier integrity, highlighting its intrinsic critical role maintenance. BECLIN1-deficient epithelial cells exhibit extensive apoptosis, impaired autophagy, stressed...
de niveau recherche, publiés ou non, émanant des établissements d'enseignement et recherche français étrangers, laboratoires publics privés.
Type 1 conventional DCs (cDC1) excel in the cross-priming of CD8+ T cells, which is crucial for orchestrating efficient immune responses against viruses or tumors. However, our understanding their physiological functions and molecular regulation has been limited by lack proper mutant mouse models allowing conditional genetic targeting. Because Xcr1 A530099j19rik (Karma/Gpr141b) genes belong to core transcriptomic fingerprint cDC1, we used them engineer two novel Cre-driver lines, Xcr1Cre...
Intraepithelial lymphocytes (IELs), including αβ and γδ T cells (T-IELs), constantly survey play a critical role in maintaining the gastrointestinal epithelium. We show that cytotoxic molecules important for defense against cancer were highly expressed by T-IELs small intestine. In contrast, abundance of colonic was dependent on microbiome displayed higher expression TCF-1/TCF7 reduced effector profile, low granzymes. Targeted deletion TCF-1 induced distinct profile colon tumor formation...
Thorough understanding of the role CD4 T cells in immunity can be greatly assisted by study responses to defined specificities. This requires knowledge Plasmodium-derived immunogenic epitopes, which only a few have been identified, especially for mouse C57BL/6 background. We recently developed TCR transgenic line, termed PbT-II, that produces CD4+ specific an MHC class II (I-Ab)-restricted Plasmodium epitope and is responsive both sporozoites blood-stage P. berghei. Here, we identify peptide...
Tissue-resident memory T cells (TRM cells) are powerful mediators of protracted adaptive immunity to infection in peripheral organs. Harnessing TRM through vaccination hence promises unprecedented potential for protection against infection. A paramount example this is malaria, a major infectious disease which traditional strategies remains challenging. Liver appear be highly protective and recent developments our knowledge the biology these have defined promising, novel their induction.Here,...
Abstract Malaria remains a major cause of mortality in the world and an efficient vaccine is best chance reducing disease burden. Vaccination strategies for liver stage that utilise injection live radiation‐attenuated sporozoites (RAS) confer sterile immunity, which mediated by CD8 + memory T cells, with liver‐resident cells (T RM ) being particularly important. We have previously described TCR transgenic mouse, termed PbT‐I, where all recognize specific peptide from Plasmodium . PbT‐I form...
Summary A successful immune response relies on a tightly regulated delivery of the right signals to cells at time. Here we show that innate and innate-like lymphocytes use two mechanisms orchestrate in time space functions conventional type 1 dendritic (cDC1) spleen. Early after murine cytomegalovirus infection, XCL1 production by with attracts red pulp cDC1 near IFN-γ-producing NK cells, generating superclusters around infected marginal zone. There, physically interact reinforcing their...
Abstract Tissue-resident immune cells reside within the gastrointestinal tract known as intraepithelial lymphocytes (T-IELs), including αβ and γδ T-IELs. This unique population of T constantly survey are critical in maintaining epithelium. We show that T-IELs various regions have distinct features. small intestine exhibit high expression cytotoxic molecules important for cancer defense while colon distinctively regulated by transcription factor, TCF-7/TCF-1, which suppresses their effector...
<h3>Background</h3> Intraepithelial lymphocytes (IELs), including αβ and γδ T cells (T-IELs) constantly survey play a critical role in maintaining the gastrointestinal (GI) epithelium. Recent reports highlight function for T-IELs defense again colorectal carcinoma (CRC).<sup>1–3</sup> <h3>Methods</h3> Tumor biopsies from patients with stage III CRC were analysed by multiplex immunohistochemistry to investigate immune cell location phenotypes correlated patient outcome data. We used mouse...
Abstract BECLIN1 is a component of Class III phosphatidylinositol 3-kinase complexes that orchestrates autophagy initiation and endocytic trafficking. Here we show intestinal epithelium-specific deletion in adult mice led to rapid fatal enteritis with compromised gut barrier integrity, highlighting its intrinsic critical role maintenance. BECLIN1-deficient epithelial cells exhibited extensive apoptosis, impaired autophagy, stressed endoplasmic reticulum mitochondria. Remaining absorptive...
Abstract Homeostasis in the gut is achieved by impeccable functioning of tissue resident immune cells that reside and closely interact with epithelium. The intestinal epithelium are predominantly lymphocytes, known as intraepithelial lymphocytes (IELs) mainly consist TCRαβ T TCRgd cells. These IELs well to sense microbes, nutrients, cellular stress but their role cancer progression has been controversial. On one hand, gd Τ primary source IL-17, an important proinflammatory cytokine aid...