A5077535787- Immune Cell Function and Interaction
- Malaria Research and Control
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- Vector-borne infectious diseases
- Multiple Myeloma Research and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Aquaculture disease management and microbiota
- Cell Adhesion Molecules Research
- HIV Research and Treatment
- Tuberculosis Research and Epidemiology
- Tryptophan and brain disorders
- Immune Response and Inflammation
Nagasaki University
2015-2024
Harvard University
2024
Boston Children's Hospital
2024
Broad Institute
2024
Peter Doherty Institute
2017
The University of Melbourne
2017
Abstract We describe an MHC class II (I-Ab)–restricted TCR transgenic mouse line that produces CD4+ T cells specific for Plasmodium species. This line, termed PbT-II, was derived from a cell hybridoma generated to blood-stage berghei ANKA (PbA). PbT-II responded all species and stages tested so far, including rodent (PbA, P. NK65, chabaudi AS, yoelii 17XNL) human (Plasmodium falciparum) parasites as well irradiated PbA sporozoites. can provide help generation of Ab infection control this...
Abstract Upon activation, specific CD4+ T cells up-regulate the expression of CD11a and CD49d, surrogate markers pathogen-specific cells. However, using T-cell receptor transgenic mice for a Plasmodium antigen, termed PbT-II, we found that activated develop not only to CD11ahiCD49dhi cells, but also CD11ahiCD49dlo during acute infection. CD49dhi PbT-II localized in red pulp spleens, expressed transcription factor T-bet produced IFN-γ, indicating they were type 1 helper (Th1)-type In...
Adaptive immune responses are critical for protection against infection with Plasmodium parasites. The metabolic state dramatically changes in T cells during activation and the memory phase. Recent findings suggest that metformin, a medication treating type-II diabetes, enhances T-cell by modulating lymphocyte metabolism. In this study, we investigated whether metformin could enhance anti-malaria immunity. Mice were infected yoelii administered metformin. Levels of parasitemia reduced...
Thorough understanding of the role CD4 T cells in immunity can be greatly assisted by study responses to defined specificities. This requires knowledge Plasmodium-derived immunogenic epitopes, which only a few have been identified, especially for mouse C57BL/6 background. We recently developed TCR transgenic line, termed PbT-II, that produces CD4+ specific an MHC class II (I-Ab)-restricted Plasmodium epitope and is responsive both sporozoites blood-stage P. berghei. Here, we identify peptide...
ABSTRACT CD8 + T cells are the major effector that protect against malaria liver-stage infection, forming clusters around Plasmodium -infected hepatocytes and eliminating parasites after a prolonged interaction with these hepatocytes. We aimed to investigate roles of specific nonspecific in cluster formation protective immunity. To this end, we used berghei ANKA expressing ovalbumin as well from transgenic mice cell receptor for (OT-I) an unrelated antigen, respectively. While...
Abstract The generation and maintenance of memory T cells are regulated by various factors, including cytokines. Previous studies have shown that IL-27 is produced during the early acute phase Plasmodium chabaudi AS (Pcc) infection inhibits development Th1-type CD4+ cells. However, whether acts directly on its receptor Plasmodium-specific or indirectly via other immune remains unclear. We aimed to determine role signaling in different cell types regulating phenotype infection. utilized...
γδ T cells facilitate the CD4 + helper 1 (Th1) cell response against Plasmodium infection by activating conventional dendritic (cDCs), although underlying mechanism remains elusive. Our study revealed that promote complete maturation and production of interleukin-12 CXCR3-ligands specifically in type cDCs (cDC1), with minimal impact on cDC2 monocyte derived DCs (Mo-DCs). During initial phase, activation temporal accumulation splenic white pulp, alongside cDC1, occur via CCR7-signaling....
Multiple immunizations using live irradiated sporozoites, the infectious plasmodial stage delivered into host skin during a mosquito bite, can elicit sterile immunity to malaria. CD8+ T cells seem play an essential role in this protective immunity, since their depletion consistently abolishes sterilizing protection several experimental models. So far, only few parasite antigens are known induce cell-dependent protection, but none of them reach levels afforded by attenuated parasites....
Malaria infection elicits both protective and pathogenic immune responses, IL-27 is a critical cytokine that regulate effector responses during infection. Here, we identified window of CD4+ T cell targeted by IL-27. Neutralization acute with Plasmodium chabaudi expanded specific cells, which were maintained at high levels thereafter. In the chronic phase, Plasmodium-specific cells in IL-27-neutralized mice consisted mainly CD127+ KLRG1- CD127- KLRG1+ subpopulations displayed distinct...
Plasmodium parasites that infect humans are highly polymorphic, and induce various infections ranging from an asymptomatic state to life-threatening diseases. However, how the differences between affect host immune responses during blood-stage infection remains largely unknown. We investigated CD4+ T-cell in mice infected with P. berghei ANKA (PbA) or chabaudi AS (Pcc) using PbT-II cells, which recognize a common epitope of these parasites. In acute phase infection, PbA-infected showed lower...
Abstract We describe an MHC II (IA b )-restricted T cell receptor (TCR) transgenic mouse line that produces CD4 + cells specific for Plasmodium species. This line, termed PbT-II, was derived from a hybridoma generated to blood-stage berghei ANKA (PbA). PbT-II responded all species and stages tested so far, including rodent (PbA, P. NK65, chabaudi AS yoelii 17XNL) human ( P . falciparum) parasites as well irradiated PbA sporozoites. can provide help generation of antibody infection control...
Malaria is a life-threatening disease caused by infection with Plasmodium parasites. The goal of developing an effective malaria vaccine yet to be reached despite decades massive research efforts. CD4+ helper T cells, CD8+ cytotoxic and γδ cells are associated immune responses both liver-stage blood-stage infection. cell-lineages protection immunopathology. In this paper, we review our knowledge cell against from studies using mouse models including current findings. Moreover, also discuss...