A5100699112- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- RNA modifications and cancer
- RNA Research and Splicing
- Melanoma and MAPK Pathways
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Cancer Research and Treatments
- Climate Change and Health Impacts
- Microtubule and mitosis dynamics
- Cancer, Hypoxia, and Metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Kinase Regulation and GTPase Signaling
- Cancer Mechanisms and Therapy
- Invertebrate Immune Response Mechanisms
- Colorectal Cancer Treatments and Studies
- Inflammatory mediators and NSAID effects
- Synthesis and biological activity
- Insect Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Heat shock proteins research
- Cell death mechanisms and regulation
Tianjin Medical University
2009-2025
East China Normal University
2013-2024
Kunming Medical University
2016-2024
Yunnan Agricultural University
2022-2023
Yunnan University
2023
Yantaishan Hospital
2022
Shanxi Medical University
2022
Qingdao University
2021
Tianjin Medical University Cancer Institute and Hospital
2016-2021
Qingdao Municipal Hospital
2021
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a broadly expressed lncRNA involved in many aspects of cellular processes. To further delineate the underlying molecular mechanism, we employed high-throughput strategy to characterize interacting proteins MALAT1 by combining RNA pull-down, quantitative proteomics, bioinformatics, and experimental validation. Our approach identified 127 potential MALAT1-interacting established highly connected interactome network consisting...
A series of novel cyclopropylamide analogues combretastatin-A4 (CA-4) were designed and synthesized. Most them had significant in vitro antiproliferative activities, particularly for compounds 7i4, 7c4, 8a4, 8c4. Moreover, compound 8c4 was also equally potent against paclitaxel resistant cancer cells. Interestingly, the different binding mechanisms from CA-4. Instead inhibiting tubulin polymerization, these CA-4 derivatives able to stimulate polymerization. Flow cytometry revealed that...
A major source of reactive oxygen species (ROS) generation is the mitochondria. By using flow cytometry mitochondrial fluorescent probe, MitoSOX Red, western blot ROS scavenger Peroxiredoxin (Prx) 3 and fluorescence immunostaining, ELISA cleaved caspases 9, TUNEL staining, we demonstrated that exposure to 100 μ M KI for 2 hours significantly increased superoxide production Prx protein expression with expressions 9. Besides, indicated dismutase (SOD) at 1000 unit/mL attenuated increase in...
BACKGROUND Long noncoding RNA (lncRNA) is a key part of class and increasing evidences have manifested that it plays significant role in the physiology pathology. The growth arrest-specific transcript 5 (GAS5) vital tumor suppressor some types cancers. However, function GAS5 lung cancer remains largely no clear. purpose current study was to identify biological non-small cell (NSCLC). MATERIAL AND METHODS To NSCLC, RT-PCR, Western Blot, Luciferase assay, immunoprecipitation assay employed...
The efficacy of epidermal growth factor receptor- targeted therapy is significantly associated with Kirsten rat sarcoma viral oncogene homolog (KRAS) and B-raf serine/threonine kinase proto-oncogene (BRAF) mutation in patients colorectal cancer (CRC), for which the standard gene testing currently performed using tumor tissue DNA. aim present study was to compare presence KRAS BRAF mutations serum exosome primary from CRC. Genomic DNA were extracted tissues 35 histologically-confirmed CRC...
Invasion and intrahepatic metastasis are major factors of poor prognosis in patients with hepatocellular carcinoma (HCC). In this study, we show that increased Src homolog collagen 3 (Shc3) expression malignant HCC cell lines associate invasion metastasis. Shc3 (N-Shc) was significantly upregulated tumors 33 patient samples as compared adjacent normal tissues. Further analysis 52 showed correlated microvascular invasion, cancer staging, prognosis. interacted vault protein, resulting...
Abstract DNA methylation, a pivotal epigenetic modification regulating gene expression, has gained significant attention as potential diagnostic biomarker for various cancers, including hepatocellular carcinoma (HCC). This study aimed to identify aberrant methylation patterns and protein alterations using integrated methodologies develop robust panel blood-based early detection of HCC. Genome-wide profiling was performed hypermethylated CpG sites genes that distinguish early-stage HCC from...
The role of Ras guanine nucleotide-releasing protein 1 (RasGRP1) in tumourigenesis has been a subject debate, and its functions clinical significance hepatocellular carcinoma (HCC) remain unknown. Here, we evaluated the expression RasGRP1 HCC determined how it contributes to cell proliferation.RasGRP1 was measured by quantitative polymerase chain reaction (qPCR) Western blotting 24 paired tissues para-tumour tissues. confirmed immunohistochemical analysis tissue microarray from independent...
Asthma belongs to chronic inflammatory respiratory diseases characterized by airway inflammation and remodeling. Circular RNAs (circRNAs) are promising therapeutic targets for various diseases, including asthma. In this work, we aim investigate the role of circular RNA Erb-B2 receptor tyrosine kinase 2 (circERBB2) during progression asthma.Human smooth muscle cells (ASMCs) were treated with platelet-derived growth factor BB (PDGF-BB) mimic cell The expression circERBB2, microRNA-98-5p...
The systematic investigation of gene mutation and expression is important to discover novel biomarkers therapeutic targets in cancers. Here, we integrated genomics, transcriptomics, proteomics, metabolomics analyze three hepatocellular carcinoma (HCC) cell lines with differential metastatic potentials. results revealed the profile prometastasis metabolism potentially associated HCC metastasis. multiomic analysis identified 12 genes variations at multiple levels from metabolic pathways,...
Sex reversal syndrome is a rare congenital condition of complete or disordered gonadal development leading to discordance between the genetic, and phenotypic sexes, including 46,XX 46,XY. The gonadoblastoma on Y‑chromosome (GBY) region associated with an increased risk developing type II germ cell tumors/cancer. present study reports unique case phenotypically normal female (age 17 years), presenting primary amenorrhea later diagnosed 46,XY sex syndrome. Following bilateral gonadectomy,...
microRNAs (miRNAs) play important roles in cancer development and progression. In this study, we explored the expression biological of miR-548b human gliomas. The glioma tissues cell lines was examined. Gain-of-function experiments were conducted to determine growth, invasiveness, tumorigenesis. Bioinformatic analysis luciferase reporter assays performed identify direct target genes for miR-548b. underexpressed lines. Re-expression significantly inhibited proliferation colony formation U87...
Liraglutide, as a glucagon-like peptide‑1 analogue, is used to treat type 2 diabetes mellitus and obesity. Previous findings have demonstrated the effects of liraglutide on adipogenesis; however, underlying mechanism involved in this process remains be elucidated. In present study, certify effect adipogenesis explore possible process, preadipocyte 3T3‑L1 cells were cultured adipocyte‑inducing medium treated with liraglutide. Subsequently, expression levels master transcription factors...
Reprogramming of cellular metabolism is one the hallmarks for cancer, in which tumor cells rewire their metabolic fluxes to generate sufficient energy and biosynthetic intermediates. Therefore, elucidating correlation between hepatocellular carcinoma (HCC) progression may provide insights into novel approaches cancer therapy.