A5100635453- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Connective Tissue Growth Factor Research
- MicroRNA in disease regulation
- Liver physiology and pathology
- IL-33, ST2, and ILC Pathways
- Macrophage Migration Inhibitory Factor
- RNA Research and Splicing
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- Cancer Research and Treatments
- Ferroptosis and cancer prognosis
- Galectins and Cancer Biology
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Epigenetics and DNA Methylation
- Cancer Immunotherapy and Biomarkers
- Cell death mechanisms and regulation
- Extracellular vesicles in disease
- Hydrogen's biological and therapeutic effects
- FOXO transcription factor regulation
- Cancer-related gene regulation
Tianjin Medical University
2005-2024
Beijing YouAn Hospital
2020-2024
Capital Medical University
2017-2024
Tianjin Medical University General Hospital
2020-2024
Institute of Human Virology
2024
Changchun Institute of Applied Chemistry
2024
Chinese Academy of Sciences
2024
University of North Carolina at Chapel Hill
2023
Capital University
2023
University of Maryland, Baltimore
2021-2023
hsa_circ_0001727 (circZKSCAN1) has been reported to be a tumor-associated circRNA by sponging microRNAs. Intriguingly, we found that circZKSCAN1 encoded secretory peptide (circZKSaa) in the liver. The present study aims elucidate potential role and molecular mechanism of circZKSaa regulation hepatocellular carcinoma (HCC) progression.The profiling datasets (RNA-seq data GSE143233 GSE140202) were reanalyzed was selected for further study. Mass spectrometry, polysome fractionation assay,...
Intracellular invasion of root cells is required for the establishment successful endosymbioses in legumes both arbuscular mycorrhizal (AM) fungi and rhizobial bacteria. In interactions a requirement entry activation common signalling pathway that includes five genes to generate calcium oscillations two perception response. Recently, it has been discovered Medicago truncatula, Vapyrin (VPY) gene essential symbiosis. Here, we show by analyses mutants same also colonization nodulation. VPY...
Abstract Molecular genetic studies suggest that FLORICAULA (FLO)/LEAFY (LFY) orthologs function to control compound leaf development in some legume species. However, loss-of-function mutations the FLO/LFY result reduction of complexity different degrees Pisum sativum and Lotus japonicus. To further understand role legumes, we studied developmental processes characterized a mutant, single leaflet1 (sgl1), from model Medicago truncatula. The sgl1 mutants exhibited strong defects development;...
Bioorthogonal reactions provide a powerful tool to manipulate biological processes in their native environment. However, the transition-metal catalysts (TMCs) for bioorthogonal catalysis are limited low atomic utilization and moderate catalytic efficiency, resulting unsatisfactory performance complex physiological Herein, sulfur-doped Fe single-atom with atomically dispersed uniform active sites fabricated serve as potent (denoted Fe-SA), which situ manipulation of cellular processes. As...
Plant leaves are diverse in their morphology, reflecting to a large degree the plant diversity natural environment. How different leaf morphology is determined not yet understood. The leguminous Medicago truncatula exhibits dissected with three leaflets at tip. We show that development of trifoliate by Cys(2)His(2) zinc finger transcription factor PALM1. Loss-of-function mutants PALM1 develop five clustered demonstrate binds specific promoter sequence and down-regulates expression M. LEAFY /...
Abstract Main conclusion An improved CRISPR/Cas9 system with the Arabidopsis UBQ10 promoter-driven Cas9 exhibits consistently high mutation efficiency in and M. truncatula. is a powerful genome editing technology that has been applied several crop species for trait improvement due to its simplicity, versatility, specificity. However, of truncatula (Mt) still challenging inconsistent. To analyze functionality two model dicot species, four different systems target phytoene desaturase ( PDS )...
Background: Hepatocellular carcinoma (HCC) is an extremely common malignant tumor with worldwide prevalence. The aim of this study was to identify potential prognostic genes and construct a competing endogenous RNA (ceRNA) regulatory network explore the mechanisms underlying development HCC. Methods: Integrated analysis used in HCC R software based on GSE14520, GSE17548, GSE19665, GSE29721, GSE60502, Cancer Genome Atlas databases. Gene Ontology Kyoto Encyclopedia Genes Genomes...
Evidence suggests that HLA-G plays a critical role in maternal immune tolerance to the fetus. However, regulation of gene expression is not well understood. Many studies have suggested progesterone may also be important suppressing response Therefore, we hypothesized this steroid hormone play regulating expression. The objective study was explore potential effects on vitro.Cultured first trimester trophoblasts and JEG-3 choriocarcinoma cells were treated with its antagonist RU486....
Lung cancer has become the top killer among malignant tumors in China and is significantly associated with somatic genetic alterations. We performed exome sequencing of 14 non–small cell lung carcinomas (NSCLCs) matched adjacent normal tissues extracted from Chinese patients. In addition to cancer–related genes (TP53, EGFR, KRAS, PIK3CA, ROS1), this study revealed “novel” not previously implicated NSCLC. Especially, matrix-remodeling 5 was second most frequently mutated gene NSCLC (first...
MicroRNAs (miRNAs) regulate gene expression by inhibiting translation of target messenger RNAs (mRNAs) through pairing with miRNA recognition elements (MREs), usually in 3'-UTRs. miRNAs are involved the pathogenesis several types cancers. Specifically, microRNA-32 (miR-32) is overexpressed colorectal carcinoma, wherein accumulating evidence indicates that it functions as an oncogene. However, function miR-32 hepatocellular carcinoma (HCC) has not been totally elucidated. In present study, we...
BACKGROUND Long noncoding RNA (lncRNA) is a key part of class and increasing evidences have manifested that it plays significant role in the physiology pathology. The growth arrest-specific transcript 5 (GAS5) vital tumor suppressor some types cancers. However, function GAS5 lung cancer remains largely no clear. purpose current study was to identify biological non-small cell (NSCLC). MATERIAL AND METHODS To NSCLC, RT-PCR, Western Blot, Luciferase assay, immunoprecipitation assay employed...
Abstract Medicago truncatula is a legume species belonging to the inverted repeat lacking clade (IRLC) with trifoliolate compound leaves. However, regulatory mechanisms underlying development of leaves in legumes remain largely unknown. Here, we report isolation and characterization fused leaf1 (fcl1) mutants M. truncatula. Phenotypic analysis suggests that FCL1 plays positive role boundary separation proximal-distal axis Map-based cloning indicates encodes class M KNOX protein harbors...
Gastric cancer (GC) is one of the common reasons cancer-related death with few biomarkers for diagnosis and prognosis. Solute carrier family 2 (facilitated glucose transporter) member 1 protein SLC2A1, also known as transporter type (GLUT1), has been associated tumor progression, metastasis, poor prognosis in many human solid tumors. However, little reported about its clinical significance biological functions GC. Here we observed a strong up-regulation SLC2A1 patients GC found that was...
Hesperidin is an abundant flavanone glycoside in citrus fruits and has been reported to possess a wide range of biological activities. However, hesperidin poor bioavailability. Here, we tested the hypothesis that hesperetin found chenpi will have better bioavailability than treatment with glucosidase-like yeast Bg1A protein increase its The results indicate pure or extract form hydrolyzed by BglA extracted from Sporobolomyces singularis expressed Escherichia coli BL21 (DE3). This...
// Ying-Chen Xu 1 , Chao-Jie Liang Dong-Xin Zhang Guan-Qun Li Xia Gao Jian-Zhu Fu Feng Jia-Jun Ji Li-Jun Guang-Ming and Ji-Xiang Wu Department of General Surgery, Beijing Tongren Hospital, Capital Medical University, 100730, China Correspondence to: Wu, email: sflian2001@163.com Li, cya_wl556@163.com Keywords: HCC, lncSHRG, SATB1, HES6, proliferation Received: March 21, 2017 Accepted: July 12, Published: August 03, ABSTRACT Hepatocellular carcinoma, one the most common cancers, leads to mass...
Transplant centers are increasingly using livers from donors aged >60 years, but long-term outcomes remain unclear. We collected data 957 adult deceased donor liver transplant recipients (conducted at our center January 2015 through October 2023). The cohort was divided into early, middle, and late era groups, each covering a 3-year interval based on date. compared 183 ≥60 years (older group [average age 64.9 ± 3.4 y]) versus 774 18 to 59 (young group), which we also after propensity score...
Endometrial regenerative cells (ERCs) have been identified to ameliorate colitis in mice; however, whether exosomes derived from ERCs (ERC-exos) own similar effects on remains unclear. Ferroptosis, an iron-dependent cell programmed death form, has reported promote inflammation UC. Thus, this study, ERC-exos can treat and regulate intestine ferroptosis will be explored. In iron, malondialdehyde (MDA) production, glutathione (GSH) synthesis, acyl-CoA synthetase long-chain family member (ACSL)...
The hepatitis C virus (HCV) core protein is critical in the development of hepatocellular carcinoma (HCC). Investigations on HCC have previously focused microRNAs, a class small non‑coding RNAs, which are crucial cancer and progression. present study aimed to investigate whether microRNA (miR)‑196a aberrantly regulated by HCV protein, miR‑196a involved regulation aberrant proliferation HCV‑HCC cells. In study, miRNA expression was detected quantitative polymerase chain reaction analysis. An...
Forkhead box F2 (FOXF2) is relatively limited to the adult lung, but its contribution non-small cell lung cancer (NSCLC) prognosis unclear.FOXF2 mRNA levels in NSCLC were lower than that paired normal tissues (P = 0.012). The FOXF2low patients had shorter survival time FOXF2high 0.024) especially stage I 0.002), chemotherapy 0.018) and < 60 age groups 0.002). Lower FOXF2 could independently predict poorer for with (HR 2.384, 95% CI 1.241-4.577; P 0.009), =4.367, =1.599-11.925; 0.004). two...
Increasing evidence has shown that abnormal expression of XPO5 is found in many human cancers and acts as an oncoprotein certain cancers. However, its functional role hepatocellular carcinoma (HCC) remains unexplored. In our study, we was highly expressed HCC, which associated with SUMO modification. Moreover, SUMOylated by SUMO2 at K125. Functional experiments revealed SUMOylation could promote MHCC97H cell proliferation, migration invasion. addition, the nuclear export pre-miR-3184...
Connective tissue growth factor (CTGF) is a possible key determinant of progressive fibrosis. Small interfering RNA (siRNA) powerful tool for silencing gene expression post-transcriptionally. Therefore, the present study aimed to determine whether synthetic siRNA target CTGF down-regulates in primary rat hepatic stellate cells (HSC) and HSC T6, and, furthermore, it prevents activation extracellular matrix (ECM) production.Primary were obtained by enzymatic perfusion liver. treated with...
Recent studies have shown that miR-564 is closely related to the development of various tumors, including breast cancer, lung cancer and glioma. However, few examined in hepatocellular carcinoma (HCC). Here, we demonstrated expression HCC tissues was lower than adjacent noncancerous associated with tumor size, number vein invasion. Bioinformatics analyses showed low levels were correlated poor prognosis. Furthermore, upregulation impaired SMCC7721 MHCC97H cell proliferation, migration...
The current chemotherapeutic outcomes for hepatocellular carcinoma (HCC) are not encouraging, and long-term survival of this patient group remains poor. Recent studies have demonstrated the utility histone deacetylase inhibitors that can disrupt cell proliferation in HCC management. However, effects droxinostat, a type inhibitor, on remain to be established.The droxinostat lines SMMC-7721 HepG2 were investigated. Histone acetylation apoptosis-modulating proteins assessed via Western blot....
Autophagy is a lysosome‑mediated cell content‑dependent degradation pathway that leads to enhanced inflammation in an uncontrolled state. This study examined the role of autophagy lipopolysaccharide (LPS)‑induced brain and effects traditional Chinese medicine ligustrazine on LPS‑induced neurocognitive impairment rats. Furthermore, molecular mechanisms by which influences impairments were explored. The production inflammatory mediators interleukin (IL)‑1β tumor necrosis factor (TNF)‑α was...