A5065166236- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Ectopic Pregnancy Diagnosis and Management
- Endometriosis Research and Treatment
- Sperm and Testicular Function
- Cancer Cells and Metastasis
- Circadian rhythm and melatonin
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Ovarian cancer diagnosis and treatment
- Cancer Immunotherapy and Biomarkers
- Cell Adhesion Molecules Research
- Ovarian function and disorders
- Endometrial and Cervical Cancer Treatments
- Pluripotent Stem Cells Research
- Cancer Research and Treatments
- Tissue Engineering and Regenerative Medicine
- Pregnancy and preeclampsia studies
- Mesenchymal stem cell research
- Reproductive Physiology in Livestock
- Reproductive biology and impacts on aquatic species
- Glycosylation and Glycoproteins Research
- Cell death mechanisms and regulation
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
2008-2022
Chengdu University of Traditional Chinese Medicine
2011-2021
Chengdu Research Base of Giant Panda Breeding
2012-2021
Core Laboratories (United States)
2018
University of Electronic Science and Technology of China
2015
CReATe Fertility Centre
2012-2013
Second Affiliated Hospital of Chengdu University of Traditional Chinese
2011-2013
University of Toronto
2004-2012
Chinese Academy of Medical Sciences & Peking Union Medical College
2010
McMaster University
1992-2009
Human leucocyte antigen (HLA) -G is expressed on trophoblast cells during pregnancy, suggesting a role in protection of the semiallogeneic fetus. Published data suggest that HLA-G protects cell against natural killer lysis. It has been hypothesized may also protect fetus by preventing allo-cytotoxic T lymphocyte (CTL) responses. To test this hypothesis, we assayed effects various concentrations purified CTL response mixed culture (MLC) system. We found > or =0.1 microg/ml suppressed allo-CTL...
Human leukocyte antigen-G (HLA-G) is a non-classical class I HLA molecule that expressed by extravillous cytotrophoblast cells. This protein may play critical role in the protection of cytotrophoblasts from maternal immune response, allowing these semi-allogeneic cells to invade uterus unimpeded. We have demonstrated diminished placental HLA-G expression associated with pre-eclampsia. In order explore fundamental mechanisms underlying this reduced pre-eclampsia, we looked for, and found...
Human leukocyte antigen‐G (HLA‐G) is a novel tumor marker and its soluble isoforms produce secretory proteins. Increased HLA‐G (sHLA‐G) levels have been reported in patients with melanoma, neuroblastoma, lymphoproliferative disorders, breast, ovarian colorectal carcinoma when compared to healthy controls or subjects benign neoplasms. The aim of this study investigate whether not plasma sHLA‐G can be used as potential biomarker for cancer diagnosis. We measured 166 early stages (CRC, n = 37),...
Putative melatonin binding sites were detected in the membrane fraction of gonadotropin-stimulated human granulosa cells using analogue 2-[125I]-iodomelatonin (125I-IML). Saturation studies and Scatchard analysis revealed presence a major site with Kd 99 pM. Guanosine triphosphate shifted receptor affinity to 380 In competition studies, rank order potency indoles for inhibition 125I-IML at these was typical receptors: 2-iodomelatonin > N-acetylserotonin 5-methoxytryptamine serotonin. Culture...
Follicular fluid samples were obtained from the largest pre-ovulatory follicle of 120 women undergoing in-vitro fertilization and examined for melatonin by enzyme-linked immunosorbent assay steroids oestradiol progesterone radioimmunoassay. The concentrations (mean +/- SE) (213.4 18.9 pmol/l) (20.1 1.1 mumol/l) in follicular during autumn winter (dark) months significantly higher than spring summer (light) months, (138.4 12.5 (11.6 0.8 mumol/l). By contrast, lower dark light (264.7 44.1...
BACKGROUND: We have previously demonstrated that progesterone has a stimulatory effect on HLA-G gene expression. Because this was abolished by the anti-progestin, RU486, we hypothesize is through receptor-mediated up-regulation of gene. The objective study to explore molecular mechanisms effect. METHODS: transient transfection chloramphenicol acetyltransferase (CAT) construct containing fragment promoter into JEG-3 choriocarcinoma cell line performed. An electrophoretic mobility shift assay...
Evidence suggests that HLA-G plays a critical role in maternal immune tolerance to the fetus. However, regulation of gene expression is not well understood. Many studies have suggested progesterone may also be important suppressing response Therefore, we hypothesized this steroid hormone play regulating expression. The objective study was explore potential effects on vitro.Cultured first trimester trophoblasts and JEG-3 choriocarcinoma cells were treated with its antagonist RU486....
The detection of HLA-G expression might serve as a clinical marker in the diagnosis or prediction outcomes for certain types carcinoma. aim this study was to determine whether has any important applications patients with esophageal squamous cell carcinoma (ESCC) by using immunohistochemical methods. We observed that protein expressed 90.9% (110/121) primary sites ESCC but not normal tissues. tumors significantly correlated histologic grade, depth invasion, nodal status, host immune response,...
Human umbilical cord-derived perivascular cells (PVCs) are a recently characterized source of mesenchymal stromal that has gained much interest in the field cellular therapeutics. However, very little is known about changes fate potential and restrictions these undergo during gestational development. This study first to examine phenotypic, molecular, functional properties trimester (FTM)-derived PVCs, outlining unique this population when compared term (TERM) counterparts. FTM- TERM-PVCs...
Abstract The functional adaptive changes in cyanide detoxification giant panda appear to be response dietary transition from typical carnivore herbivorous bear. We tested the absorption of contained bamboo/bamboo shoots with a feeding trial 20 adult pandas. determined total content bamboo and panda’s feces, levels urinary thiocyanate tissue rhodanese activity using color reactions spectrophotometer. Rhodanese expression liver kidney at transcription translation were measured real-time RT-PCR...
Melatonin concentrations and aromatase stimulating activity were determined in human seminal plasma correlated with sperm density motility. Aromatase was an vitro rat granulosa cell system melatonin by radioimmunoassay. Compared to normal semen, lower azoospermic individuals, while higher oligospermic samples. positively a negative correlation found between progression. These findings suggest that low production is associated bioactivity plasma; may have effect upon both