A5072401668- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Neonatal Respiratory Health Research
- Cardiac electrophysiology and arrhythmias
- Reproductive System and Pregnancy
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Ion channel regulation and function
- Tissue Engineering and Regenerative Medicine
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- CRISPR and Genetic Engineering
- Malaria Research and Control
- MicroRNA in disease regulation
- Air Quality and Health Impacts
- Blood properties and coagulation
- Advanced Glycation End Products research
- Renal and related cancers
- Neuroscience and Neuropharmacology Research
- 3D Printing in Biomedical Research
- Estrogen and related hormone effects
- Nitric Oxide and Endothelin Effects
- Receptor Mechanisms and Signaling
- RNA Interference and Gene Delivery
- Zebrafish Biomedical Research Applications
- Mosquito-borne diseases and control
- Cancer-related molecular mechanisms research
Kangwon National University
2016-2025
Nongwoo Bio (South Korea)
2022-2025
Kangwon National University Hospital
2015-2024
Hallym University Kangnam Sacred Heart Hospital
2024
Organ Technologies (Japan)
2024
Korea Testing & Research Institute
2023
Severance Hospital
2023
CHA Bundang Medical Center
2023
CHA University
2023
Korea Institute of Science and Technology
2008-2022
Liver transplantation is the last resort for liver failure patients. However, due to shortage of donor organs, bioengineered generated from decellularized whole scaffolds and induced pluripotent stem cell (iPSC)-derived hepatocytes (iPSC-Heps) being studied as an alternative approach treat disease. Nevertheless, there has been no report on both interaction iPSC-Heps with a extracellular matrix (ECM) analysis recellularized into scaffolds. In this study, we produced porcine iPSC-Heps, which...
Abstract Detailed understanding of the pathogenesis and development effective therapies for pulmonary fibrosis (PF) have been hampered by lack in vitro human models that recapitulate disease pathophysiology. In this study, we generated alveolar organoids (AOs) derived from pluripotent stem cells (hPSCs) use as an PF model drug efficacy evaluation. Stepwise direct differentiation hPSCs into epithelial mimicking developmental cues a temporally controlled manner was used to generate...
Growing evidence links prenatal exposure to particulate matter (PM2.5) with reduced lung function and incidence of pulmonary diseases in infancy childhood. However, the underlying biological mechanisms how PM2.5 affects lungs are incompletely understood, which explains lack an ideal vitro development model. Human pluripotent stem cells (hPSCs) have been successfully employed for developmental toxicity evaluations due their unique ability differentiate into any type cell body. In this study,...
The Plasmodium vivax reticulocyte-binding protein (RBP) family was identified based on the annotation of adhesive ligands in P. genome. Reticulocyte-specific interactions with PvRBPs (PvRBP1 and PvRBP2) were previously reported. falciparum homologue 4 (PfRh4, a PvRBP1) observed to possess erythrocyte-binding activity via complement receptor 1 erythrocyte surface. However, mechanisms are unclear because large molecular mass PvRBP1 (>326 kDa) difficulty associated vitro cultivation. In present...
Cadmium (Cd), a major component of cigarette smoke, disrupts the normal functions airway cells and can lead to development various pulmonary diseases such as chronic obstructive disease (COPD). However, molecular mechanisms involved in Cd-induced are poorly understood. Here, we identified cluster genes that altered response Cd exposure human bronchial epithelial (BEAS-2B) demonstrated ER stress inflammation mediated via CCAAT-enhancer-binding proteins (C/EBP)-DNA-damaged-inducible transcript...
The oxidative stress and cellular apoptosis by environmental factor including cigarette smoke induces alveolar airway remodeling leading to chronic obstructive pulmonary disease (COPD). Recently, the receptor for advanced glycan end products (RAGE) which is highly expressed in epithelium emerging as a biomarker COPD susceptibility or progression. However, it still remains unknown how RAGE plays role extract (CSE)-exposed human type II epithelial cell line. Therefore, we determined efficacy...
A major limitation in anti-tuberculosis drug screening is the lack of reliable and scalable models for homogeneous human primary macrophage cells non-cancer origin. Here we report a modified protocol generating populations macrophage-like from embryonic stem cells. The induced macrophages, referred to as iMACs, presented similar transcriptomic profiles characteristic immunological features classical macrophages were permissive viral bacterial infection, particular Mycobacterium tuberculosis...
Inflammation is an important priming event in the pathogenesis of pulmonary diseases, including chronic obstructive disease (COPD). Increasing evidence indicates that microRNAs (miRNAs) contribute to COPD by regulating inflammatory response. Therefore, it necessary investigate novel molecular targets without any validation samples airway inflammation. The aim our study reveal miRNAs can influence for and examine underlying mechanism inflammation.We identified downregulation miR-181a-2-3p...
Mechanisms that govern hematopoietic lineage specification, as opposed to the expansion of committed progenitors, from human pluripotent stem cells (hPSCs) have yet be fully defined. Here, we show within family genes called inhibitors differentiation (ID), ID1 and ID3 negatively regulate transition lineage-specified hemogenic progenitors during hematopoiesis both embryonic (hESCs) induced cell (hiPSCs). Upon induction hPSCs, levels transcripts rapidly increase, peaking at stage precursor...
Human umbilical cord-derived perivascular cells (PVCs) are a recently characterized source of mesenchymal stromal that has gained much interest in the field cellular therapeutics. However, very little is known about changes fate potential and restrictions these undergo during gestational development. This study first to examine phenotypic, molecular, functional properties trimester (FTM)-derived PVCs, outlining unique this population when compared term (TERM) counterparts. FTM- TERM-PVCs...
There is no therapy currently available that influences the natural history of disease progression in patients with chronic obstructive pulmonary (COPD). Although stem cell considered a potential therapeutic option COPD, there are clinical trials proving definitive effects COPD. Recently, it was reported pioglitazone might potentiate cells heart or liver disease. To test capacity pretreatment for emphysema repair, we evaluated pioglitazone-pretreated human adipose-derived mesenchymal (ASCs)...
Asherman's syndrome (AS) is characterized by intrauterine adhesions or fibrosis resulting from scarring inside the endometrium. AS associated with infertility, recurrent miscarriage, and placental abnormalities. Although mesenchymal stem cells show therapeutic promise for treatment of AS, molecular mechanisms underlying its pathophysiology remain unclear. We ascertained that mice like human patients suffer extensive fibrosis, oligo/amenorrhea, infertility. Human perivascular (hPVSCs)...
Chronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality worldwide. Single-cell RNA sequencing (scRNA-seq) provides gene expression profiles at the single-cell level. Hence, we evaluated in peripheral blood patients with COPD. Peripheral samples from seven healthy controls eight COPD were obtained this study. The 10X Genomics Chromium Instrument cDNA synthesis kit utilized to generate barcoded library for single cell RNA-sequencing. We compared scRNA-seq...
Macrophages play a key role in host defense and inflammation, with polarization ranging from pro-inflammatory M1 to anti-inflammatory M2 states. However, effective modulation of macrophage polarity via nucleotide delivery is challenging. This study developed polyethyleneimine-modified carboxyl quantum dots (QDP) as biocompatible carrier for small RNA modulate polarization. QDP-mediated miR-10a (QDP/miR-10a) rebalanced alleviated uterine inflammation fibrosis mouse model Asherman's syndrome...
The etiology of chronic and lethal interstitial lung disease, termed idiopathic pulmonary fibrosis (IPF), remains unidentified. IPF induces pathological scarring that results in rigidity impairs gas exchange, eventually resulting premature mortality. Recent findings indicate deubiquitinating enzymes play a key role stabilizing fibrotic proteins contribute to fibrosis. ubiquitin-specific protease 11 (USP11) promotes pro-fibrotic proteins, its expression elevated tissue samples from patients...
Recent advancements in automated scoliosis diagnosis using X-ray imaging have focused on detecting spinal curvature through angle measurements, but they often lack systematically standardized clinical terminology schemes. To address these limitations, we propose an end-to-end pipeline that detects individual vertebrae and Cobb angles including reports of structured medical terms. The performance the proposed was evaluated SpineWeb public dataset, achieving state-of-the-art results with a...