A5072345714- Malaria Research and Control
- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- Complement system in diseases
- Vector-borne infectious diseases
- vaccines and immunoinformatics approaches
- Drug Transport and Resistance Mechanisms
- Parasites and Host Interactions
- HIV Research and Treatment
- Hepatitis B Virus Studies
- Computational Drug Discovery Methods
- Trypanosoma species research and implications
- Insect Resistance and Genetics
- Toxoplasma gondii Research Studies
- Aquaculture disease management and microbiota
- Viral Infections and Vectors
- Transgenic Plants and Applications
- Parasite Biology and Host Interactions
- Bacteriophages and microbial interactions
- Bird parasitology and diseases
- Research on Leishmaniasis Studies
- Studies on Chitinases and Chitosanases
- Drug-Induced Hepatotoxicity and Protection
- CRISPR and Genetic Engineering
- Hepatitis Viruses Studies and Epidemiology
Ehime University
2016-2025
CellFree Sciences (Japan)
2021-2025
Kobe Medical Center
2021
Osaka University
2018-2020
Gulu University
2018-2020
Juntendo University Hospital
2020
Juntendo University
2018
Tokyo Women's Medical University
2018
National Institute of Advanced Industrial Science and Technology
2018
The University of Tokyo
2018
Abstract The development of effective malaria vaccines and immune biomarkers is a high priority for control elimination. Ags expressed by merozoites Plasmodium falciparum are likely to be important targets human immunity promising vaccine candidates, but very few have been studied. We developed an approach assess Ab responses comprehensive repertoire merozoite proteins investigate whether they protective Abs. 91 recombinant proteins, located on the surface or within invasion organelles,...
ABSTRACT Loop-mediated isothermal amplification (LAMP), a novel nucleic acid method, was developed for the clinical detection of four species human malaria parasites: Plasmodium falciparum , P. vivax malariae and ovale . We evaluated sensitivity specificity LAMP in comparison with results microscopic examination nested PCR. showed limit (analytical sensitivity) 10 copies target 18S rRNA genes 100 genus detected parasites 67 68 microscopically positive blood samples (sensitivity, 98.5%) 3 53...
One of the major bottlenecks in malaria research has been difficulty recombinant protein expression. Here, we report application wheat germ cell-free system for successful production proteins. For proof principle, Pfs25, PfCSP, and PfAMA1 proteins were chosen. These genes contain very high A/T sequences are also difficult to express as In our system, native codon-optimized versions Pfs25 produced equal amounts PfCSP without any codon optimization expressed. The products soluble, with yields...
Falciparum malaria is initiated when Anopheles mosquitoes transmit the Plasmodium sporozoite stage during a blood meal. Irradiated sporozoites confer sterile protection against subsequent infection in animal models and humans. This level of unmatched by current recombinant vaccines. However, live-attenuated vaccine approach faces formidable obstacles, including development accurate, reproducible attenuation techniques. We tested whether falciparum could be attenuated at early liver genetic...
Because ≈90% of malaria cases occur in Africa, emergence artemisinin-resistant Plasmodium falciparum Africa poses a serious public health threat. To assess parasites Uganda during 2014-2016, we used the recently developed ex vivo ring-stage survival assay, which estimates ring-stage-specific P. susceptibility to artemisinin. We conducted 4 cross-sectional surveys artemisinin sensitivity Gulu, Uganda. Among 194 isolates, rates (ratio viable drug-exposed drug-nonexposed controls) were high...
ABSTRACT Recently, there has been a renewed interest in the development of transmission-blocking vaccines (TBV) against Plasmodium falciparum malaria. While several candidate TBVs have reported, studies directly comparing them functional assays are limited. To this end, recombinant proteins TBV candidates Pfs25, Pfs230, and PfHAP2 were expressed wheat germ cell-free expression system. Outbred CD-1 mice immunized twice with antigens. Two weeks after second immunization, IgG levels measured by...
Abstract Malaria transmission-blocking vaccines (TBVs) target the development of Plasmodium parasites within mosquito, with aim preventing malaria transmission from one infected individual to another. Different vaccine platforms, mainly protein-in-adjuvant formulations delivering leading candidate antigens, have been developed independently and reported varied activities (TBA). Here, recombinant chimpanzee adenovirus 63, ChAd63 modified vaccinia virus Ankara, MVA, expressing AgAPN1,...
Abstract Antibodies against P . falciparum merozoites fix complement to inhibit blood-stage replication in naturally-acquired and vaccine-induced immunity; however, specific targets of these functional antibodies their importance protective immunity are unknown. Among malaria-exposed individuals, we show that complement-fixing more strongly correlated with than growth quantified using the current reference assay for merozoite vaccine evaluation. We identify antigen-specific associated...
ABSTRACT Transmission-blocking vaccines are one strategy for controlling malaria, whereby sexual-stage parasites inhibited from infecting mosquitoes by human antibodies. To evaluate whether the recently cloned Plasmodium vivax proteins Pvs25 and Pvs28 candidates a transmission-blocking vaccine, molecules were expressed in yeast as secreted recombinant proteins. Mice vaccinated with these adsorbed to aluminum hydroxide developed strong antibody responses against immunogens, although Pvs28,...
Completed genome sequences and stage-specific transcriptomes of the intraerythrocytic developmental cycle Plasmodium vivax offers opportunity to profile immune responses against P. infection using innovative screening approaches. To detect blood proteins, we applied a protein array technology screen sera malaria patients. Herein, set genes from stage was cloned In-Fusion cloning method expressed by wheat germ cell-free system. A total 94 open reading frames (ORFs) were 89 (95%, 89/94)...
Background. How antimalarial antibodies are acquired and maintained during pregnancy boosted after reinfection with Plasmodium falciparum vivax is unknown. Methods. A nested case-control study of 467 pregnant women (136 Plasmodium-infected cases 331 uninfected control subjects) in northwestern Thailand was conducted. Antibody levels to P. merozoite antigens the pregnancy-specific PfVAR2CSA antigen were determined at enrollment (median 10 weeks gestation) throughout until delivery. Results....
Autophagy is a membrane-mediated degradation process, which governed by sequential functions of Atg proteins. Although proteins are highly conserved in eukaryotes, protozoa possess only partial set Nonetheless, almost all have the complete factors belonging to Atg8 conjugation system, namely, Atg3, Atg4, Atg7, and Atg8. Here, we report biochemical properties subcellular localization protein human malaria parasite Plasmodium falciparum (PfAtg8). PfAtg8 expressed during intra-erythrocytic...
Plasmodium falciparum is transmitted to a new host after completing its sexual cycle within mosquito. Developing vaccines against the parasite stages critical component in fight malaria. We are targeting multiple proteins of P. which found only on surfaces forms and where antibodies these have been shown block progression parasite's life mosquito thus transmission next human host. successfully produced region Pfs230 antigen our plant-based transient-expression system evaluated this vaccine...
Significance Slow-clearing artemisinin-resistant malaria parasites are now well established in the Greater Mekong Subregion. This large multinational therapy efficacy study incorporating clinical data, molecular drug-resistance markers, and immune profiling aimed to understand how variations population levels of naturally acquired malarial immunity affect slow-clearing phenotype, emergence artemisinin resistance-associated mutations, assessment geographical spread resistance. We found that...
We used the loop-mediated isothermal amplification (LAMP) method developed by our group for malaria diagnosis with genus-specific and species-specific primers four human parasites at a field clinic in comparison standard microscopy. Among 110 blood samples collected from Thailand, LAMP detected 59 of 60 positive microscopy (sensitivity = 98.3%) none 50 microscopy-negative (specificity 100%). Negative predictive value (NPV) (PPV) were 98% 100%, respectively. These results indicate that is an...
ABSTRACT The aim of a malaria transmission-blocking vaccine is to block the development parasites in mosquito and thus prevent subsequent infection human host. Previous studies have demonstrated that gametocyte/gamete surface protein Pfs230 can induce immunity evaluated Escherichia coli -produced as candidate. In this study, we used wheat germ cell-free expression system produce N-terminal fragments activity antisera raised against recombinant protein. rabbit reacted cultured gametocytes...