A5091528692- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Mosquito-borne diseases and control
- Viral gastroenteritis research and epidemiology
- Toxin Mechanisms and Immunotoxins
- Influenza Virus Research Studies
- Insect Resistance and Genetics
- Bacillus and Francisella bacterial research
- SARS-CoV-2 and COVID-19 Research
- Insect symbiosis and bacterial influences
- Fungal and yeast genetics research
- Probiotics and Fermented Foods
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Response and Inflammation
- Malaria Research and Control
- Bacterial Genetics and Biotechnology
- Viral Infections and Outbreaks Research
- Microbial Natural Products and Biosynthesis
- Mitochondrial Function and Pathology
- 14-3-3 protein interactions
- Rabies epidemiology and control
- SARS-CoV-2 detection and testing
- Food Industry and Aquatic Biology
Fraunhofer USA
2021-2024
Fraunhofer USA Center for Molecular Biotechnology
2013-2023
University of Pennsylvania
2022
Applied Biotechnology Institute
2005-2006
California Polytechnic State University
2006
Salk Institute for Biological Studies
1999
Noble Research Institute
1999
University of Leicester
1992-1993
University of Kent
1992
Plants offer an alternative to microbial fermentation and animal cell cultures for the production of recombinant proteins. For protein pharmaceuticals, plant systems are inherently safer than native even sources. In addition, post-translational modifications, such as glycosylation, which cannot be achieved with bacterial fermentation, can accomplished using plants. The main advantage foreseen is reduced costs. should have a particular proteins produced in bulk, industrial enzymes, product...
The Arabidopsis chlorophyll a/b binding protein (CAB) gene underexpressed 1 (cue1) mutant underexpresses light-regulated nuclear genes encoding chloroplast-localized proteins. cue1 also exhibits mesophyll-specific chloroplast and cellular defects, resulting in reticulate leaves. Both the underexpression leaf cell morphology phenotypes are dependent on light intensity. In this study, we determine that CUE1 encodes plastid inner envelope phosphoenolpyruvate/phosphate translocator (PPT) define...
Antibodies (inhibitors) developed by hemophilia B patients against coagulation factor IX (FIX) are challenging to eliminate because of anaphylaxis or nephrotic syndrome after continued infusion. To address this urgent unmet medical need, FIX fused with a transmucosal carrier (CTB) was produced in commercial lettuce (Simpson Elite) cultivar using species specific chloroplast vectors regulated endogenous psbA sequences. CTB-FIX (∼1 mg/g) lyophilized cells stable proper folding, disulfide bonds...
Malaria continues to be one of the world's most devastating infectious tropical diseases, and alternative strategies prevent infection disease spread are urgently needed. These include development effective vaccines, such as malaria transmission blocking vaccines (TBV) directed against proteins found on sexual stages Plasmodium falciparum parasites present in mosquito midgut. The Pfs25 protein, which is expressed surface gametes, zygotes ookinetes, has been a primary target for TBV...
Malaria transmission blocking vaccines (TBVs) are considered an effective means to control and eventually eliminate malaria. The Pfs25 protein, expressed predominantly on the surface of sexual sporogonic stages Plasmodium falciparum including gametes, zygotes ookinetes, is one primary targets for TBV. It has been demonstrated that plants effective, highly scalable system production recombinant proteins, virus-like particles (VLPs). We engineered VLPs (Pfs25-CP VLP) comprising fused Alfalfa...
To advance a novel concept of debulking virus in the oral cavity, primary site viral replication, virus-trapping proteins CTB-ACE2 were expressed chloroplasts and clinical-grade plant material was developed to meet FDA requirements. Chewing gum (2 g) containing cells up 17.2 mg ACE2/g dry weight (11.7% leaf protein), have physical characteristics taste/flavor like conventional gums, no protein lost during compression. efficiently (>95%) inhibited entry lentivirus spike or VSV-spike...
Plasmodium falciparum is transmitted to a new host after completing its sexual cycle within mosquito. Developing vaccines against the parasite stages critical component in fight malaria. We are targeting multiple proteins of P. which found only on surfaces forms and where antibodies these have been shown block progression parasite's life mosquito thus transmission next human host. successfully produced region Pfs230 antigen our plant-based transient-expression system evaluated this vaccine...
Background Influenza A viruses are of major concern for public health, causing worldwide epidemics associated with high morbidity and mortality. Vaccines critical protection against influenza, but given the recent emergence new strains pandemic potential, some limitations current production systems, there is a need approaches vaccine development. Objective To demonstrate immunogenicity protective efficacy plant‐produced influenza antigens. Method We engineered, using A/Wyoming/3/03 (H3N2) as...
Recently, we have reported [1,2] on a subunit influenza vaccine candidate based the recombinant hemagglutinin protein from A/Indonesia/05/2005 (H5N1) strain of virus, produced it using 'launch vector'-based transient expression technology in Nicotiana benthamiana, and demonstrated its immunogenicity pre-clinical studies. Here, present results first-in-human, Phase 1 randomized, double-blind, placebo-controlled study designed to investigate safety, reactogenicity three escalating dose levels...
Malaria is a serious and sometimes fatal mosquito-borne disease caused by protozoan parasite. Each year, it estimated that over one million people are killed malaria, yet the preventable treatable. Developing vaccines against parasite critical component in fight malaria these can target different stages of pathogen's life cycle. We targeting sexual stage proteins P. falciparum which found on surface reproductive cells present mosquito gut. Antibodies block progression parasite's cycle...
Abstract Transmission-blocking vaccines have the potential to be key contributors malaria elimination. Such elicit antibodies that inhibit parasites during their development in Anopheles mosquitoes, thus breaking cycle of transmission. To date, characterization humoral responses Plasmodium falciparum transmission-blocking vaccine candidate Pfs25 has largely been conducted pre-clinical models. Here, we present molecular analyses human antibody generated a clinical trial evaluating...