A5002063963- Microfluidic and Capillary Electrophoresis Applications
- Oral and gingival health research
- Microfluidic and Bio-sensing Technologies
- Malaria Research and Control
- S100 Proteins and Annexins
- Mitochondrial Function and Pathology
- Advanced Biosensing Techniques and Applications
- Mosquito-borne diseases and control
- Immune Response and Inflammation
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- ATP Synthase and ATPases Research
- RNA Interference and Gene Delivery
- Oral microbiology and periodontitis research
- Adipose Tissue and Metabolism
- Antimicrobial Peptides and Activities
- RNA and protein synthesis mechanisms
- Magnetic and transport properties of perovskites and related materials
- Innovative Microfluidic and Catalytic Techniques Innovation
- Rare-earth and actinide compounds
- Aquaculture disease management and microbiota
- Adipokines, Inflammation, and Metabolic Diseases
- Gastrointestinal disorders and treatments
- Platelet Disorders and Treatments
- Metabolism and Genetic Disorders
Shimane University
2017-2024
Shimane University Hospital
2020-2023
National Institute of Advanced Industrial Science and Technology
2013-2022
University Gastroenterology
2021
Shimane Prefectural Central Hospital
2019-2020
Juntendo University
2018
Osaka University
2009-2018
The University of Tokyo
2018
Ehime University
2018
Tokyo Women's Medical University
2018
Because ≈90% of malaria cases occur in Africa, emergence artemisinin-resistant Plasmodium falciparum Africa poses a serious public health threat. To assess parasites Uganda during 2014-2016, we used the recently developed ex vivo ring-stage survival assay, which estimates ring-stage-specific P. susceptibility to artemisinin. We conducted 4 cross-sectional surveys artemisinin sensitivity Gulu, Uganda. Among 194 isolates, rates (ratio viable drug-exposed drug-nonexposed controls) were high...
The frequencies of the softest polar optic phonons tetragonal SrTiO3 are accurately determined using high-resolution hyper-Raman spectroscopy on oriented-domain crystals. splitting soft mode in phase is quantitatively related to 105 K structural transition, i.e., rotation oxygen octahedra and associated macroscopic strains. In addition quantum fluctuations, these distortions found be essential preventing ferroelectric transition.
Clinical and biochemical markers of periodontal disease have been used for precise objective diagnosis inflammation. Interleukin 1beta (IL-1beta) prostaglandin E2 (PGE2), inflammatory factors, levels in gingival crevicular fluid (GCF) patients with are elevated studied as markers. The calprotectin, a leukocyte protein, body fluids some diseases raised. Recently, we detected calprotectin GCF its concentrations pockets were higher than those healthy crevices. In this study, investigated the...
Human epidermis and epithelium serve as physiologic barriers to protect against noxious infectious agents. Contributing the defense infection, epithelial cells express antimicrobial peptides (AMPs). The expression of AMPs in keratinocytes is generally regulated directly by bacteria indirectly proinflammatory cytokines. Bacteria may also regulate AMP inducing keratinocyte autonomous cytokine, interleukin‐1 α (IL‐1 ). To test hypothesis that be cell cytokines, we investigated effect IL‐1 on...
Magnetization and initial permeability measurements were done on the ferromagnetic shape memory alloys ${\text{Ni}}_{2}{\text{Mn}}_{1\ensuremath{-}x}{\text{Cu}}_{x}\text{Ga}$ $(0\ensuremath{\le}x\ensuremath{\le}0.4)$. On basis of results, phase diagram in temperature-concentration plane was determined for this alloy system. The is spanned by paramagnetic austenite (I), martensite (II), (III), (IV), premartensite (V). It found that magnetostructural transitions between phases I IV can occur...
Kido J, Bando M, Hiroshima Y, Iwasaka H, Yamada K, Ohgami N, Nambu T, Kataoka Yamamoto Shinohara Sagawa I, Nagata T. Analysis of proteins in human gingival crevicular fluid by mass spectrometry. J Periodont Res 2012; 47: 488–499. © 2012 John Wiley & Sons A/S Background and Objective: Gingival is a bodily transuded from periodontal tissues into the crevice pocket, contains many species components. Proteins have been studied as markers for diseases. Mass spectrometric analysis used...
Current diagnostic tests such as glycemic indicators have limitations for early detection of impaired glucose tolerance (IGT), which leads to diabetes. Oxidative stress induced by various oxidants in a random and destructive manner is considered play an important role the pathophysiology number human disorders diseases tolerance. We developed improved method measurement vivo lipid peroxidation, where presence 8-iso-prostaglandin F2α (8-iso-PGF2α), hydroxyoctadecadienoic acids (HODEs),...
Lipopolysaccharide from Porphyromonas gingivalis (P-LPS), an important pathogenic bacterium, is closely associated with inflammatory destruction of periodontal tissues. P-LPS induces the release cytokines and local factors cells, stimulates osteoclastic-cell differentiation, causes alveolar bone resorption. However, effect on osteoblastic-cell differentiation remains unclear. In this study, we investigated extract prepared by hot-phenol-water method, primary fetal rat calvaria (RC) which...
Cyclosporin A (CsA) is used as an immunosuppressive agent and its prominent side effect the induction of fibrous gingival overgrowth. The purpose this study was to investigate CsA on type I collagen metabolism in gingiva rats fed a powdered diet either containing or lacking CsA. Immunohistochemical analysis revealed that more prevalent connective tissue CsA-treated than those control days 15, 30, 55 after start feeding. Total RNAs were isolated from mandibular molar 0, 3, 8, 55. Quantitative...
The voltage-dependent anion channel (VDAC) is a pore-forming protein expressed in the outer membrane of eukaryotic mitochondria. Three isoforms it, i.e., VDAC1, VDAC2, and VDAC3, are known to be mammals; however, question as which main isoform mitochondria still unanswered. To address this question, we first prepared standard VDACs by using bacterial expression system raised various antibodies against them synthetic peptides immunogens. Of three bacterially VDAC isoforms, VDAC3 showed faster...
Calprotectin, a major cytosol protein of leukocytes, exists in plasma and other body fluids healthy human subjects. Since the calprotectin concentration rises markedly some inflammatory diseases including rheumatoid arthritis, this has been thought to be marker disease. Recently, we identified dental calculus gingival crevicular fluid (GCF), found that GCF from patients with periodontitis was significantly higher than In present study, association level volume, index (GI), levels biochemical...
A plasmonic chip was applied to live cancer cell imaging. The epithelial adhesion molecule (EpCAM) is a surface marker that can be used classify breast lines into distinct differentiation states. EpCAM and the nuclei of two kinds living cells, MDA-MB231 MCF-7, were stained with allophycocyanin (APC)-labeled anti-EpCAM antibody 4',6-diamidino-2-phenylindole (DAPI), respectively, cells scattered on either (metal-coated wavelength-scale grating substrate) or control glass slide. Multicolor...
Objectives: Calprotectin is a cytosolic protein with antibacterial action in leukocytes and its level increases some inflammatory diseases, including periodontal rheumatoid arthritis ulcerative colitis. Recently, we found that the lipopolysaccharide of Porphyromonas gingivalis (P‐LPS) induced calprotectin release from human neutrophils. P‐LPS, major virulence factor pathogens, known to induce production cytokines through CD14, Toll‐like receptor (TLR) nuclear κB (NF‐κB). In present study,...
Background: Substance P (SP) is a multifunctional neuropeptide that transmits pain signals, regulates the immune system, and may modulate emotional stress. SP stimulates bone resorption activity of osteoclasts, level in gingival crevicular fluid correlated with degree periodontal inflammation. However, exact roles metabolism diseases are poorly understood. To elucidate effect stress on metabolism, we investigated osteoblastic cell differentiation presence lipopolysaccharide from...
Background Malaria is one of the major human infectious diseases in many endemic countries. For prevention spread malaria, it necessary to develop an early, sensitive, accurate and conventional diagnosis system. Methods Findings A cell microarray chip was used detect for malaria-infected erythrocytes. The chip, with 20,944 microchambers (105 µm width 50 depth), made from polystyrene, formation monolayers erythrocytes observed. Cultured Plasmodium falciparum strain 3D7 examine potential...
Hiroshima Y, Bando M, Inagaki Mihara C, Kataoka Murata H, Shinohara Nagata T, Kido J. Resistin in gingival crevicular fluid and induction of resistin release by Porphyromonas gingivalis lipopolysaccharide human neutrophils. J Periodont Res 2012; 47: 554–562. © 2012 John Wiley & Sons A/S Background Objective: is an adipocytokine that induces insulin resistance predominantly expressed adipocytes peripheral blood mononuclear cells. expression increases inflammatory diseases as well diabetes...
We have previously found that fasting plasma levels of totally assessed 10- and 12-(Z,E)-hydroxyoctadecadienoic acid (HODE) correlated well with glycated hemoglobin (HbA1c) glucose during oral tolerance tests (OGTT); these were determined via liquid chromatography-mass spectrometry after reduction saponification. However, 12-(Z,E)-HODE alone cannot perfectly detect early impaired (IGT) and/or insulin resistance, which ultimately lead to diabetes. In this study, we randomly recruited healthy...
Individual drug treatment may select resistant parasites in the human body, a process termed vivo selection. Some single nucleotide polymorphisms Plasmodium falciparum chloroquine-resistance transporter (pfcrt) and multidrug resistance gene 1 (pfmdr1) genes have been reportedly selected after artemether–lumefantrine treatment. However, there is paucity of data regarding selection P. Kelch propeller domain (pfkelch13) polymorphisms, responsible for artemisinin-resistance Asia, six putative...
There are no reports regarding the efficacy of sodium-glucose cotransporter 2 inhibitor (SGLT2i) and dipeptidyl peptidase 4 (DPP4i) administrations in nonalcoholic fatty liver disease (NAFLD) patients without type diabetes mellitus. The purpose this study was to evaluate those drugs such patients. NAFLD mellitus were enrolled single center double-blind randomized prospective study, allocated receive either dapagliflozin or teneligliptin for 12 weeks. Laboratory variables body compositions...