A5100781827- Pluripotent Stem Cells Research
- Liver physiology and pathology
- Pancreatic function and diabetes
- 3D Printing in Biomedical Research
- Liver Disease Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Hepatocellular Carcinoma Treatment and Prognosis
- Ginseng Biological Effects and Applications
- Esophageal and GI Pathology
- Nanoplatforms for cancer theranostics
- Phagocytosis and Immune Regulation
- MicroRNA in disease regulation
- Tracheal and airway disorders
- Diverse Approaches in Healthcare and Education Studies
- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- Extracellular vesicles in disease
- CAR-T cell therapy research
- Liver Disease and Transplantation
- Esophageal Cancer Research and Treatment
- MRI in cancer diagnosis
- Innovations in Medical Education
- RNA Interference and Gene Delivery
Gangnam Severance Hospital
2025
Yonsei University
2004-2025
Korea University
2016-2025
International Vaccine Institute
2025
Korea Research Institute of Bioscience and Biotechnology
2023-2025
Chungnam National University
2024
University of Ulsan
2002-2024
Asan Medical Center
2002-2024
Ulsan College
2002-2024
University of Missouri
2024
Transcription factors (TFs) are major trans-acting in transcriptional regulation. Therefore, elucidating TF-target interactions is a key step toward understanding the regulatory circuitry underlying complex traits such as human diseases. We previously published reference interaction database for humans-TRRUST (Transcriptional Regulatory Relationships Unraveled by Sentence-based Text mining)-which was constructed using sentence-based text mining, followed manual curation. Here, we present...
Biotin is a promising targeting unit for carcinoma cell lines and the avidin–biotin technology an exceedingly flexible tool pretargeting vector targeting. Thus, biotin conjugation attractive choice achieving site-specific sensing, labeling, delivery.
A major challenge in photodynamic cancer therapy (PDT) is avoiding PDT-induced hypoxia, which can lead to recurrence and progression through activation of various angiogenic factors significantly reduce treatment outcomes. Reported here an acetazolamide (AZ)-conjugated BODIPY photosensitizer (AZ-BPS) designed mitigate the effects PDT-based hypoxia by combining benefits anti-angiogenesis with PDT. AZ-BPS showed specific affinity aggressive cells (MDA-MB-231 cells) that overexpress carbonic...
Activation of apoptosis, the cell death machinery, in tumor cells by organelle-specific delivery antitumor theranostic agent is utmost challenge cancer therapy. Herein, we developed a highly efficient mitochondria-targeting prodrug 7 that contained two molecules drug 5′-deoxy-5-fluorouridine and an apoptotic marker ethidium for self-monitoring intrinsic (mitochondrial) apoptosis after its activation cells. Theranostic was activated endogenously produced mitochondrial-overexpressed H2O2...
Abstract Allergic asthma is characterized by airway inflammation initiated adaptive immune responses to aeroallergens. Recent data suggest that severe may be a different form of rather than an increase in symptoms and innate LPS can modulate allergens. In this study, we evaluated the hypothesis exposure doses induces asthma. Our study showed neutrophilic IFN-γ expression were higher induced sputum from patients mild moderate asthmatics. Animal experiments indicated allergen sensitization...
Mitochondria are organelles that readily susceptible to temperature elevation. We selectively delivered a coumarin-based fluorescent iron oxide nanoparticle, Mito-CIO, the mitochondria. Upon 740 nm laser irradiation, intracellular of HeLa cells was elevated by 2.1 °C within 5 min when using and treatment resulted in better hyperthermia more cytotoxicity than treated with coumarin (CIO), which missing mitochondrial targeting unit. further confirmed these results tumor xenograft mouse model....
Abstract In early embryonic development, spatial gradients of diffusible signaling molecules play important roles in controlling differentiation cell types or arrays diverse tissues. Thus, the concentration exogenous cytokines growth factors at any given time is crucial to formation an enriched population a desired type from primitive stem cells vitro. Microfluidic technology has proven very useful creation cell-friendly microenvironments. Such techniques are, however, currently limited few...
This study aimed to evaluate the therapeutic efficacy and safety of repeated transarterial chemoembolization (TACE) with additional radiation therapy (RT) in hepatocellular carcinoma (HCC) portal vein (PV) invasion.We performed survival analysis consecutive HCC patients PV invasion according treatment modalities after stratification by degree liver function retrospectively.During 2005, 281 were newly diagnosed have at our institution. Repeated TACE or chemoinfusion (TACI) was 202 (71.9%) RT...
Significance Hemophilia A, a genetic bleeding disorder, is often caused by chromosomal inversions that involve portion of the blood coagulation factor VIII ( F8 ) gene encodes one key enzymes in clotting. In this study, we developed known as transcription activator-like effector nucleases (TALENs) cleave DNA targeted manner to invert 140-kbp segment spans human induced pluripotent stem cells (iPSCs) create hemophilia A model cell line. addition, reverted inverted back its normal orientation...
Nanoparticle-based nucleic acid conjugates (NP-NACs) hold great promise for theragnostic (diagnostic and therapeutic) applications. However, several limitations have hindered the realization of their full potential in clinical treatment cancer other diseases. In diagnosis, NP-NACs, combined with conventional optical sensing systems, been applied detection
Background/Aims: Stereotactic body radiation therapy (SBRT) is used as an alternative ablative treatment in patients with hepatocellular carcinoma (HCC) not suitable for curative treatments. The purpose of this prospective study was to evaluate the long-term efficacy SBRT small (≤5 cm) HCCs.Methods: A phase II, single-arm clinical trial on HCCs conducted at academic tertiary care center. planned dose 45 Gy a fraction size 15-Gy over 3 consecutive days. primary endpoint 2-year local control...
Abstract Detailed understanding of the pathogenesis and development effective therapies for pulmonary fibrosis (PF) have been hampered by lack in vitro human models that recapitulate disease pathophysiology. In this study, we generated alveolar organoids (AOs) derived from pluripotent stem cells (hPSCs) use as an PF model drug efficacy evaluation. Stepwise direct differentiation hPSCs into epithelial mimicking developmental cues a temporally controlled manner was used to generate...
Human in vitro hepatic models that faithfully recapitulate liver function are essential for successful basic and translational research. A limitation of current models, which extensively used drug discovery toxicity testing, is the loss metabolic due to low expression activity cytochrome P450 (CYP450) enzymes. Here, we aimed generate human pluripotent stem cell-derived organoids (hHOs) with a high ability. We established two-step protocol produce hHOs from cells long-term expansion testing....
Abstract Background The generation of liver organoids recapitulating parenchymal and non-parenchymal cell interplay is essential for the precise in vitro modeling diseases. Although different types multilineage (mLOs) have been generated from human pluripotent stem cells (hPSCs), assembly concurrent differentiation multiple individual mLOs remain a major challenge. Particularly, most studies focused on vascularization host tissue after transplantation vivo. However, relatively little...
The utility of electro-responsive smart materials has been limited by bubble generation (hydrolysis) during application electrical fields and biocompatibility issues. Here we describe the design a device that overcomes these limitations combining material properties, new concepts, microtechnology. 4-hydroxybutyl acrylate (4-HBA) was used as backbone hydrogel material, its actuating behavior, bending force, elasticity were extensively characterized function size acrylic acid concentration. To...
The development of targeted and effective theranostic (therapeutic diagnostic) chemotherapeutic agents is highly desirable for precise diagnosis treatment cancer. To realize this goal, we developed a cancer-targeting enzyme-triggered prodrug 1, containing 7-ethyl-10-hydroxycamptothecin (SN-38), well-known anticancer drug, which inhibits topoisomerase I in the cell nucleus; hydroquinone as an moiety; biotin cancer targeting unit. Enzyme-triggered 1 selectively targets cells subsequently...
Recent studies have shown that defined factors could lead to the direct conversion of fibroblasts into induced hepatocyte-like cells (iHeps). However, reported efficiencies are very low, and underlying mechanism hepatic reprogramming is largely unknown. Here, we report iHeps a stepwise transition involving erasure somatic memory, mesenchymal-to-epithelial transition, induction cell fate in sequential manner. Through screening for additional potentially enhance kinetics, found c-Myc Klf4 (CK)...